Multifrequency electron paramagnetic resonance characterization of PpoA, a CYP450 fusion protein that catalyzes fatty acid dioxygenation

Journal of the American Chemical Society

Volumn 133, Issue 23, 2011, Pages 9052-9062

  Fielding, Alistair J ^a   Brodhun, Florian ^c   Koch, Christian ^c   Pievo, Roberta ^a   Denysenkov, Vasyl ^d   Feussner, Ivo ^c   Bennati, Marina ^a,b  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

^b UNIVERSITY OF GÖTTINGEN   (Germany)

^c UNIVERSITY OF GÖTTINGEN   (Germany)

^d GOETHE UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ACID RADICALS; ASPERGILLUS NIDULANS; DIOXYGENASES; ENDOR SPECTROSCOPY; ENHANCED T; FUSION PROTEINS; G-VALUES; HEME DOMAIN; HEME PEROXIDASE; HIGH FREQUENCY EPR; HYPERFINE COUPLING; MULTIFREQUENCY ELECTRON PARAMAGNETIC RESONANCE; PARAMAGNETIC CENTERS; PARAMAGNETIC INTERMEDIATES; PULSED EPR; RADICAL CHEMISTRY; REACTION MECHANISM; REACTION STEPS; SECONDARY METABOLISM; SPECTRAL SIMULATIONS; SPECTROSCOPIC EVIDENCE; SUBSTANTIAL REDUCTION; TETRAMERIC ENZYMES; THIOLATES; TIME-SCALES;

AMINO ACIDS; ENZYMES; IRON COMPOUNDS; LINOLEIC ACID; METABOLISM; PARAMAGNETIC MATERIALS; PARAMAGNETIC RESONANCE; PARAMAGNETISM; PORPHYRINS; REACTION INTERMEDIATES; RESONANCE; SPECTROSCOPY;

ELECTRON SPIN RESONANCE SPECTROSCOPY;

AMINO ACID; CYTOCHROME P450; DIOXYGENASE; FATTY ACID; FERRIC ION; HEMOPROTEIN; HISTIDINE; HYBRID PROTEIN; MONOMER; PPOA PROTEIN; RADICAL; TETRAMER; TYROSYL DEXTRO ALANYLGLYCYL N METHYLPHENYLALANINE PROPIONOHYDRAZIDE; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CHEMICAL REACTION; CONTROLLED STUDY; ELECTRON SPIN RESONANCE; OXYGENATION; PROTEIN ANALYSIS; SPECTROSCOPY;

BIOCATALYSIS; CYTOCHROME P-450 ENZYME SYSTEM; ELECTRON SPIN RESONANCE SPECTROSCOPY; FATTY ACIDS; FREE RADICALS; HEME; HISTIDINE; MUTATION; OXYGEN; RECOMBINANT FUSION PROTEINS; TYROSINE;

EID: 79958833110     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja202207t     Document Type: Article

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