Isolation of recombinant secretory proteins by limited induction and quantitative harvest

BioTechniques

Volumn 24, Issue 2, 1998, Pages 188-192

  Rosenberg, Helene F ^a  

^a NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

SECRETORY PROTEIN;

ARTICLE; BACTERIAL MEMBRANE; CARBOXY TERMINAL SEQUENCE; CYTOLYSIS; GENETIC RECOMBINATION; NONHUMAN; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN ISOLATION; PROTEIN SYNTHESIS REGULATION;

BACTERIA (MICROORGANISMS);

EID: 0031940586     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98242bm03     Document Type: Article

References (10)

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2. Blight, M.A. and I.B. Holland. 1994. Heterologous protein secretion and the versatile Escherichia coli haemolysin translocator. Trends Biotechnol. 12:450-455.
3. Humphreys, D.P., N. Weir, A. Lawson, A. Mountain and P.A. Lund. 1996. Co-expression of human protein disulphide isomerase (PDI) can increase the yield of an antibody Fab' fragment expressed in Escherichia coli. FEBS Lett. 380:194-197.
4. Jose, J., J. Kramer, T. Klauser, J. Pohlner and T.F. Meyer. 1996. Absence of periplasmic DsbA oxidoreductase facilitates export of cysteine-containing passenger proteins to the Escherichia coli cell surface via the IgA beta autotransporter pathway. Gene 178:107-110.
5. Kaderbhai, N., A. Karim, W. Hankey, G. Jenkins, J. Venning and M.A. Kaderbhai. 1997. Glycine-induced extracellular secretion of a recombinant cytochrome expressed in Escherichia coli. Biotechnol. Appl. Biochem. 25:53-61.
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7. Perez-Perez, J., C. Martinez-Caja, J.L. Barbero and J. Gutierrez. 1995. DnaK/DnaJ supplementation improves the periplasmic production of human granulocyte-colony stimulating factor in Escherichia coli. Biochem. Biophys. Res. Commun. 210:524-529.
8. Rosenberg, H.F. 1995. Recombinant human eosinophil cationic protein: ribonuclease activity is not essential for cytotoxicity. J. Biol. Chem. 270:7876-7881.
9. Rosenberg, H.F. and K.D. Dyer. 1995. Eosinophil cationic protein and eosinophil-derived neurotoxin. Evolution of novel function in a primate ribonuclease gene family. J. Biol. Chem. 270:21539-21544.
10. Rosenberg, H.F., D.G. Tenen and S.J. Ackerman. 1989. Molecular cloning of the human eosinophil-derived neurotoxin: a member of the ribonuclease gene family. Proc. Natl. Acad. Sci. USA 86:4460-4464.