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0028825817
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Gene fusion expression systems in Escherichia coli
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LaVallie ER, McCoy JM. Gene fusion expression systems in Escherichia coli. of special interest Curr Opin Biotechnol. 6:1995;501-506 Reviews some current gene fusion expression systems in use in E. coli with the exception of the ubiquitin fusion system.
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LaVallie, E.R.1
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0024593537
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The tails of ubiquitin precursors are ribosomal proteins whose fusion to ubiquitin facilitates ribosome biogenesis
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Finley D, Bartel B, Varshavsky A. The tails of ubiquitin precursors are ribosomal proteins whose fusion to ubiquitin facilitates ribosome biogenesis. Nature. 338:1989;394-401.
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Nature
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Finley, D.1
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4
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0025991456
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Cloning and functional analysis of the ubiquitin-specific protease gene Ubp1 of Saccharomyces cerevisiae
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Tobias JW, Varshavsky A. Cloning and functional analysis of the ubiquitin-specific protease gene Ubp1 of Saccharomyces cerevisiae. J Biol Chem. 266:1991;12021-12028.
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Tobias, J.W.1
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0026457302
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Ubiquitin-specific proteases of Saccharomyces cerevisiae
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Baker RT, Tobias JT, Varshavsky A. Ubiquitin-specific proteases of Saccharomyces cerevisiae. J Biol Chem. 267:1992;23364-23375.
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Baker, R.T.1
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Varshavsky, A.3
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6
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0027427249
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The yeast DOA4 gene encodes a deubiquitinating enzyme related to a product of the human tre-2 oncogene
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Papa F, Hochstrasser M. The yeast DOA4 gene encodes a deubiquitinating enzyme related to a product of the human tre-2 oncogene. Nature. 366:1993;313-319.
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Papa, F.1
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7
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0024521520
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Increasing gene expression in yeast by fusion to ubiquitin
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Ecker DJ, Stadel JM, Butt TR, Marsh JA, Monia BP, Powers DA, Gorman JA, Clark PE, Warren F, Shatzman A, Crooke ST. Increasing gene expression in yeast by fusion to ubiquitin. J Biol Chem. 264:1989;7715-7719.
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Ecker, D.J.1
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Monia, B.P.5
Powers, D.A.6
Gorman, J.A.7
Clark, P.E.8
Warren, F.9
Shatzman, A.10
Crooke, S.T.11
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8
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0024308204
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Cloning and expression of a yeast ubiquitin-protein cleaving activity in Escherichia coli
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Miller HI, Henzel WJ, Ridgway JB, Kuang W-J, Chisolm V, Liu C-C. Cloning and expression of a yeast ubiquitin-protein cleaving activity in Escherichia coli. Bio-Technology. 7:1989;698-704.
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Miller, H.I.1
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Chisolm, V.5
Liu C-C6
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10
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0029119522
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A proteolytic pathway that recognizes ubiquitin as a degradation signal
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of special interest. Describes a proteolytic pathway in yeast responsible for degrading ubiquitin fusion proteins where the ubiquitin cannot be cleaved. Emphasizes the importance of not preventing ubiquitin cleavage in eukaryotic hosts.
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Johnson ES, Ma PCM, Ota I, Varshavsky A. A proteolytic pathway that recognizes ubiquitin as a degradation signal. of special interest J Biol Chem. 270:1995;17442-17456 Describes a proteolytic pathway in yeast responsible for degrading ubiquitin fusion proteins where the ubiquitin cannot be cleaved. Emphasizes the importance of not preventing ubiquitin cleavage in eukaryotic hosts.
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J Biol Chem
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Johnson, E.S.1
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11
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0024402418
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High-level expression and in vivo processing of chimeric ubiquitin fusion proteins in Saccharomyces cerevisiae
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Sabin EA, Lee-Ng CT, Shuster JR, Barr PJ. High-level expression and in vivo processing of chimeric ubiquitin fusion proteins in Saccharomyces cerevisiae. Bio-Technology. 7:1989;705-709.
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Sabin, E.A.1
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Barr, P.J.4
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12
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0026691270
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Characterization of recombinant human insulin-like growth factor binding proteins 4, 5, and 6 produced in yeast
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Kiefer MC, Schmid C, Waldvogel M, Schlapfer I, Futo E, Masiarz FR, Green K, Barr PJ, Zapf J. Characterization of recombinant human insulin-like growth factor binding proteins 4, 5, and 6 produced in yeast. J Biol Chem. 267:1992;12692-12699.
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Kiefer, M.C.1
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Futo, E.5
Masiarz, F.R.6
Green, K.7
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Zapf, J.9
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13
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0028142992
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Protein expression using cotranslational fusion and cleavage of ubiquitin. Mutagenesis of the glutathione-binding site of human pi class glutathione S-transferase
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Baker RT, Smith SA, Marano R, McKee J, Board PG. Protein expression using cotranslational fusion and cleavage of ubiquitin. Mutagenesis of the glutathione-binding site of human pi class glutathione S-transferase. J Biol Chem. 269:1994;25381-25386.
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Baker, R.T.1
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McKee, J.4
Board, P.G.5
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14
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0029155342
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Sequencing and expression of a cDNA for human glutathione synthetase
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of special interest. Describes a cotranslational ubiquitin fusion/cleavage system in E. coli using Ubp2p to cleave ubiquitin.
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Gali RR, Board PG. Sequencing and expression of a cDNA for human glutathione synthetase. of special interest Biochem J. 310:1995;353-358 Describes a cotranslational ubiquitin fusion/cleavage system in E. coli using Ubp2p to cleave ubiquitin.
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Biochem J
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Gali, R.R.1
Board, P.G.2
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15
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0029870896
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Purification and characterization of a recombinant human theta-class glutathione transferase (GSTT2-2)
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of special interest. Describes a cotranslational ubiquitin fusion/cleavage system in E. coli using Ubp1p to cleave ubiquitin.
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Tan KL, Board PG. Purification and characterization of a recombinant human theta-class glutathione transferase (GSTT2-2). of special interest Biochem J. 315:1996;727-732 Describes a cotranslational ubiquitin fusion/cleavage system in E. coli using Ubp1p to cleave ubiquitin.
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Biochem J
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Tan, K.L.1
Board, P.G.2
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16
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0028965728
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Mutations causing coagulation factor XIII subunit A deficiency: Characterization of the mutant proteins after expression in yeast
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Coggan M, Baker R, Miloszewski K, Woodfield G, Board P. Mutations causing coagulation factor XIII subunit A deficiency: characterization of the mutant proteins after expression in yeast. Blood. 9:1995;2455-2460.
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Coggan, M.1
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Board, P.5
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17
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0027202203
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Augmentation of protein production by a combination of the T7 RNA polymerase system and ubiquitin fusion: Overproduction of the human DNA repair protein, ERCC1, as a ubiquitin fusion protein in Escherichia coli
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Koken MH, Odijk HH, Van Duin M, Fornerod M, Hoeijmakers JH. Augmentation of protein production by a combination of the T7 RNA polymerase system and ubiquitin fusion: overproduction of the human DNA repair protein, ERCC1, as a ubiquitin fusion protein in Escherichia coli. Biochem Biophys Res Commun. 195:1993;643-653.
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Koken, M.H.1
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Hoeijmakers, J.H.5
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18
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0028242750
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Tyrosinase production in recombinant E. coli containing trp promoter and ubiquitin sequence
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Han K, Hong J, Lim HC, Kim CH, Park Y, Cho JM. Tyrosinase production in recombinant E. coli containing trp promoter and ubiquitin sequence. Ann NY Acad Sci. 721:1994;30-42.
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Han, K.1
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19
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0028834866
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Purification of human matrilysin produced in Escherichia coli and characterization using a new optimized fluorogenic peptide substrate
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of special interest. Describes a ubiquitin fusion system where Ubp-mediated removal of ubiquitin is not required because the fused protein is a zymogen. Cleavage is activated after purification of the intact fusion protein.
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Welch AR, Holman CM, Browner MF, Gehring MR, Kan CC, Van-Wart HE. Purification of human matrilysin produced in Escherichia coli and characterization using a new optimized fluorogenic peptide substrate. of special interest Arch Biochem Biophys. 324:1995;59-64 Describes a ubiquitin fusion system where Ubp-mediated removal of ubiquitin is not required because the fused protein is a zymogen. Cleavage is activated after purification of the intact fusion protein.
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Arch Biochem Biophys
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Welch, A.R.1
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20
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0024840348
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Expression of functional chicken oviduct progesterone receptors in yeast (Saccharomyces cerevisiae)
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Mak P, McDonnell DP, Weigel NL, Schrader WT, O'Malley BW. Expression of functional chicken oviduct progesterone receptors in yeast (Saccharomyces cerevisiae). J Biol Chem. 264:1989;21613-21618.
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Mak, P.1
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21
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0026636455
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Human estrogen receptor regulation in a yeast model system and studies on receptor agonists and antagonists
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22
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0027450565
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Synthesis of human parathyroid-hormone-related protein(1-141) in Saccharomyces cerevisiae. A correct amino-terminal processing vital for the hormone's biological activity is obtained by an ubiquitin fusion protein approach
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Rian E, Jemtland R, Olstad OK, Gordeladze JO, Gautvik KM. Synthesis of human parathyroid-hormone-related protein(1-141) in Saccharomyces cerevisiae. A correct amino-terminal processing vital for the hormone's biological activity is obtained by an ubiquitin fusion protein approach. Eur J Biochem. 213:1993;641-648.
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23
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0026598125
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A novel, highly regulated, rapidly inducible system for the expression of chicken progesterone receptor, cPRA, in Saccharomyces cerevisiae
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Poletti A, Weigel NL, McDonnell DP, Schrader WT, O'Malley BW, Conneely OM. A novel, highly regulated, rapidly inducible system for the expression of chicken progesterone receptor, cPRA, in Saccharomyces cerevisiae. Gene. 114:1992;51-58.
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Poletti, A.1
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24
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0028082492
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Correct in vivo processing of a chimeric ubiquitin - Proapolipoprotein A-I fusion protein in baculovirus-infected insect cells
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Moguilevsky N, Guillaume JP, Varsalona F, Bulinckx M, Bollen A. Correct in vivo processing of a chimeric ubiquitin - proapolipoprotein A-I fusion protein in baculovirus-infected insect cells. J Biotechnol. 32:1994;39-43.
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Moguilevsky, N.1
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25
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0008595755
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Ubiquitin fusion augments the yield of cloned gene products in Escherichia coli
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Butt TR, Jonnalagadda S, Monia BP, Sternberg EJ, Marsh JA, Stadel JM, Ecker DJ, Crooke ST. Ubiquitin fusion augments the yield of cloned gene products in Escherichia coli. Proc Natl Acad Sci USA. 86:1989;2540-2544.
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26
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0029116080
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Kan, C.C.4
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27
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High-level expression and efficient recovery of ubiquitin fusion proteins from Escherichia coli
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of outstanding interest. Describes the scale-up of the ubiquitin fusion technique to the 10 litre fermenter, and demonstrates the high yields that can be obtained from this system. Also reports a further yield enhancement following a heat shock during fermentation.
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Pilon AL, Yost P, Chase TE, Lohnas GL, Bentley WE. High-level expression and efficient recovery of ubiquitin fusion proteins from Escherichia coli. of outstanding interest Biotechnol Prog. 12:1996;331-337 Describes the scale-up of the ubiquitin fusion technique to the 10 litre fermenter, and demonstrates the high yields that can be obtained from this system. Also reports a further yield enhancement following a heat shock during fermentation.
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Balzi E, Choder M, Chen W, Varshavsky A, Goffeau A. Cloning and functional analysis of the arginyl-tRNA - protein transferase gene ATE1 of Saccharomyces cerevisiae. J Biol Chem. 265:1990;7464-7471.
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