Expression and purification of human antimicrobial peptide, dermcidin, in Escherichia coli

Protein Expression and Purification

Volumn 45, Issue 2, 2006, Pages 269-274

  Čipáková, Ingrid ^a   Gašperík, Juraj ^b   Hostinová, Eva ^b  

^a CPN spol s ro ^*  (Czech Republic)

^b INSTITUTE OF CHEMISTRY   (Slovakia)

Author keywords

Anionic antimicrobial peptide; Dermcidin; Recombinant production

Indexed keywords

ANTIINFECTIVE AGENT; DERMCIDIN; HYBRID PROTEIN; PEPTIDE;

AMINO ACID SEQUENCE; ARTICLE; CELL INCLUSION; CHEMISTRY; ESCHERICHIA COLI; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGICAL EXAMINATION; MOLECULAR CLONING; MOLECULAR GENETICS;

AMINO ACID SEQUENCE; ANTI-INFECTIVE AGENTS; CLONING, MOLECULAR; ESCHERICHIA COLI; HUMANS; INCLUSION BODIES; MICROBIAL SENSITIVITY TESTS; MOLECULAR SEQUENCE DATA; PEPTIDES; RECOMBINANT FUSION PROTEINS;

CANDIDA ALBICANS; ESCHERICHIA COLI; LISTERIA MONOCYTOGENES; SALMONELLA TYPHIMURIUM; STAPHYLOCOCCUS AUREUS;

EID: 31044435481     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.07.002     Document Type: Article

References (12)

1. B. Schittek, R. Hipfel, B. Sauer, J. Bauer, H. Kalbacher, S. Stevanovic, M. Schirle, K. Schroeder, N. Blin, F. Meier, G. Rassner, and C. Garbe Dermcidin: a novel human antibiotic peptide secreted by sweat glands Nat. Immunol. 2 2001 1133 1137
2. D. Porter, S. Weremowicz, K. Chin, P. Seth, A. Keshaviah, J. Lahti-Domenici, Y.K. Bae, C.L. Monitto, A. Merlos-Suarez, J. Chan, C.M. Hulette, A. Richardson, C.C. Morton, J. Marks, M. Duyao, R. Hruban, E. Gabrielson, R. Gelman, and K.P.A. Polyak A neural survival factor is a candidate oncogene in breast cancer Proc. Natl. Acad. Sci. USA 19 2003 10931 10936
3. T. Flad, R. Bogumil, J. Tolson, B. Schittek, C. Garbe, M. Deeg, C.A. Mueller, and H. Kalbacher Detection of dermcidin-derived peptides in sweat by ProteinChip technology J. Immunol. Methods 1 2002 53 62
4. M.M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 72 1976 248 254
5. R.I. Lehrer, M. Rosenman, S.S.L. Harwig, R. Jackson, and P. Eisenhauer Ultrasensitive assays for endogenous antimicrobial polypeptides J. Immunol. Methods 137 1991 167 173
6. A. Kuliopulos, D. Shortle, and P. Talalay Isolation and sequencing of the gene encoding delta 5-3-ketosteroid isomerase of Pseudomonas testosteroni: overexpression of the protein Proc. Natl. Acad. Sci. USA 84 1987 8893 8897
7. L. Peng, Z. Xu, X. Fang, F. Wang, S. Yang, and P. Cen High-level expression of soluble human β-defensin-2 in Escherichia coli Process Biochem. 39 2004 2199 2205
8. Y.H. Yang, G.G. Zheng, X.J. Zhang, Z.Y. Cao, Q. Rao, and K.F. Wu Expression of bioactive recombinant GSLL-39, a variant of human antimicrobial peptide LL-37, in Escherichia coli Protein Express. Purif. 37 2004 229 235
9. Y.P. Lai, Y.F. Peng, Y. Zuo, J. Li, J. Huang, L.F. Wang, and Z.R. Wu Functional and structural characterization of recombinant dermcidin-1L, a human antimicrobial peptide Biochem. Biophys. Res. Commun. 328 2005 243 250
10. I. Čipáková, E. Hostinová, J. Gašperík, and V. Velebný High-level expression and purification of a recombinant hBD-1 fused to LMM protein in Escherichia coli Protein Express. Purif. 37 2004 207 212
11. I. Čipáková, and E. Hostinová Production of the human-beta-defensin using Saccharomyces cerevisiae as a host Protein Pept. Lett. 12 2005 551 554
12. M. Murakami, T. Ohtake, R.A. Dorschner, B. Schittek, C. Garbe, and R.L. Gallo Cathelicidin anti-microbial peptide expression in sweat, an innate defense system for the skin J. Invest. Dermatol. 5 2002 1090 1095