Efficient secretion of biologically active recombinant OB protein (leptin) in Escherichia coli, purification from the periplasm and characterization

Protein Expression and Purification

Volumn 12, Issue 2, 1998, Pages 249-258

  Guisez, Yves ^a   Faché, Ina ^a   Campfield, L Arthur ^b   Smith, Françoise J ^b   Farid, Adrienne ^c   Plaetinck, Geert ^a   Van Der Heyden, José ^a   Tavernier, Jan ^a   Fiers, Walter ^d   Burn, Paul ^b   Devos, René ^a  

^a Roche Research   (Belgium)

^b Hoffman La Roche Department of Metabolic Diseases   (United States)

^c HOFFMANN LA ROCHE INC   (United States)

^d GHENT UNIVERSITY   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

BACULOVIRUS; BINDING SITE; CHROMATOGRAPHY; CYTOPLASM; GEL FILTRATION; HYBRID; ION EXCHANGE; LEPTIN; LIPOPROTEIN; MEMBRANE PROTEIN; MOUSE; OBESITY; POLYPEPTIDE; PURIFICATION; RECOMBINANT PROTEIN; RIBOSOME; SECRETION;

ESCHERICHIA COLI; INSECTA; UNIDENTIFIED BACULOVIRUS;

EID: 0032030174     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1997.0836     Document Type: Article

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