Determining protease substrate selectivity and inhibition by label-free supramolecular tandem enzyme assays

Journal of the American Chemical Society

Volumn 133, Issue 19, 2011, Pages 7528-7535

  Ghale, Garima ^a   Ramalingam, Vijayakumar ^b   Urbach, Adam R ^b   Nau, Werner M ^a  

^a JACOBS UNIVERSITY BREMEN   (Germany)

^b TRINITY UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACRIDINE ORANGE; ANALYTICAL METHOD; AROMATIC AMINO ACID; CLEAVAGE PRODUCTS; CONTINUOUS MONITORING; CUCURBIT[7]URIL; ENZYMATIC CLEAVAGE; ENZYME ASSAYS; EXOPEPTIDASE ACTIVITY; FLUORESCENT DYES; HIGH AFFINITY; INHIBITION CONSTANTS; LABEL FREE; LABEL-FREE APPROACH; MACROCYCLES; N-TERMINALS; PHENYLALANINE RESIDUES; PROTEASE ACTIVITIES; PROTEASE INHIBITION; REAL TIME; SEQUENCE SPECIFICITY; STEREOSPECIFICITY; SUBSTRATE PEPTIDES; SUBSTRATE SELECTIVITY; THERMOLYSIN;

AMINO ACIDS; ASSAYS; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; PEPTIDES; SULFUR COMPOUNDS; SUPRAMOLECULAR CHEMISTRY;

ENZYME INHIBITION;

ACRIDINE ORANGE; CUCURBIT[7]URIL; ENKEPHALIN; EXOPEPTIDASE; MACROCYCLIC COMPOUND; PHENYLALANINE; PHOSPHORAMIDON; PROTEINASE; THERMOLYSIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BINDING AFFINITY; ENZYME ACTIVITY; ENZYME ASSAY; FLUORESCENCE SPECTROSCOPY; PROTEIN DEGRADATION; STEREOSPECIFICITY; SUPRAMOLECULAR CHEMISTRY;

ENZYME ASSAYS; FLUORESCENT DYES; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; KINETICS; MOLECULAR STRUCTURE; PEPTIDE HYDROLASES; PROTEASE INHIBITORS; SUBSTRATE SPECIFICITY;

EID: 79955881748     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja2013467     Document Type: Article

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