Enzymatic and structural approaches of the thermolysin mechanism in glycerol-containing media

Annals of the New York Academy of Sciences

Volumn 864, Issue , 1998, Pages 458-462

  Pauthe, Emmanuel ^a   Dauchez, Manuel ^b   Berry, Hugues ^a   Berjot, Maurice ^b   Monti, Jean Pierre ^c   Alix, Alain J P ^b   Larreta Garde, Veronique ^d  

^a UNIVERSITÉ DE TECHNOLOGIE DE COMPIÈGNE   (France)

^b INSERM   (France)

^c UNIVERSITÉ DE PICARDIE JULES VERNE   (France)

^d UNIVERSITÉ DE CERGY PONTOISE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BUFFER; GLYCEROL; THERMOLYSIN; TRIPEPTIDE;

CONFERENCE PAPER; CULTURE MEDIUM; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SUBSTRATE; ENZYME SUBSTRATE COMPLEX; HYDROLYSIS; MOLECULAR INTERACTION;

EID: 0032419791     PISSN: 00778923     EISSN: None     Source Type: Book Series    
DOI: 10.1111/j.1749-6632.1998.tb10359.x     Document Type: Conference Paper

References (8)

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2. 2. IZQUIERDO, M. C. & R. L. STEIN. 1990. Mechanism studies of thermolysin. J. Am. Chem. Soc. 112: 6054-6062.
3. 3. EIJSINK, V. G. H., O. R. VELTMAN, W. AUKEMA, G. VRIEND & G. VENEMA. 1995. Structural determinants of the stability of thermolysin-like proteinases. Struct. Biol. 2: 374-379.
4. 4. HANGAUER, D. G., A. F. MONZINGO & B. W. MATTHEWS. 1984. An interactive computer graphics study of thermolysin-catalyzed peptide cleavage and inhibition by N-carboxymethyl dipeptides. Biochemistry 23: 5730-5741.
5. 5. HOLLAND, D. R., A. C. HAUSRATH, D. JUERS & B. W. MATTHEWS. 1995. Structural analysis of zinc substitutions in the active site of thermolysin. Protein Sci. 4: 1955-1965.
6. 6. LIGNE, T., E. PAUTHE, J. P. MONTI, G. GACEL & V. LARRETA-GARDE. 1996. Additional data about thermolysin specificity in buffer-and glycerol-containing media. Biochim. Biophys. Acta 1337: 143-148.
7. 7. GEKKO, K. & S. N. TIMASHEFF. 1981. Mechanism of protein stabilization by glycerol: preferential hydration in glycerol-water mixture. Biochemistry 20: 4667-4676.
8. 8. BALTORA-ROSSET, S., D. MARTY, F. Y. DUPRADEAU, E. PAUTHE, V. LARRETA-GARDE, G. GACEL & J. P. MONTI. 1997. Microenvironment effect on structure of synthetic substrate of thermolysin. Lett. Pept. Sci. 4: 391-395.