Protein phosphatase PP6 is required for homology-directed repair of DNA double-strand breaks

Cell Cycle

Volumn 10, Issue 9, 2011, Pages 1411-1419

  Zhong, Jianing ^a   Liao, Ji ^a   Liu, Xin ^b   Wang, Pei ^a   Liu, Jinping ^a   Hou, Wenya ^a   Zhu, Bingtao ^a   Yao, Lu ^b   Wang, Jinsheng ^c   Li, Jing ^a   Stark, Jeremy M ^d   Xie, Yuntao ^b   Xu, Xingzhi ^a  

^a CAPITAL NORMAL UNIVERSITY   (China)

^b PEKING UNIVERSITY CANCER HOSPITAL AND INSTITUTE   (China)

^c Department of Surgical Oncology   (China)

^d CITY OF HOPE NATIONAL MEDICAL CENTER   (United States)

Author keywords

DNA double strand break; Homology directed repair; PP6; Protein phosphatase; H2AX

Indexed keywords

DOUBLE STRANDED DNA; HISTONE H2AX; PHOSPHATASE; PP6 ENZYME; PP6C ENZYME; PP6R3 ENZYME; PPR2 ENZYME; UNCLASSIFIED DRUG;

ARTICLE; BREAST DISEASE; BREAST TUMOR; CHROMATIN IMMUNOPRECIPITATION; CONTROLLED STUDY; DNA DAMAGE; DOUBLE STRANDED DNA BREAK; ENZYME ACTIVITY; HUMAN; HUMAN CELL; HUMAN TISSUE; PROTEIN DEPLETION; PROTEIN PHOSPHORYLATION;

EID: 79955611119     PISSN: 15384101     EISSN: 15514005     Source Type: Journal    
DOI: 10.4161/cc.10.9.15479     Document Type: Article

References (32)

1. Harper JW, Elledge SJ. The DNA damage response: Ten years after. Mol Cell 2007; 28:739-45. (Pubitemid 350217064)
2. Ohnishi T, Mori E, Takahashi A. DNA double-strand breaks: Their production, recognition and repair in eukaryotes. Mutat Res 2009; 669:8-12.
3. Moorhead GB, Trinkle-Mulcahy L, Ulke-Lemee A. Emerging roles of nuclear protein phosphatases. Nat Rev Mol Cell Biol 2007; 8:234-44.
4. Eichhorn PJ, Creyghton MP, Bernards R. Protein phosphatase 2A regulatory subunits and cancer. Biochim Biophys Acta 2009; 1795:1-15.
5. Rogakou EP, Boon C, Redon C, Bonner WM. Megabase chromatin domains involved in DNA double- strand breaks in vivo. J Cell Biol 1999; 146:905-16.
6. Rogakou EP, Pilch DR, Orr AH, Ivanova VS, Bonner WM. DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139. J Biol Chem 1998; 273:5858-68. (Pubitemid 28124064)
7. Chowdhury D, Keogh MC, Ishii H, Peterson CL, Buratowski S, Lieberman J. gamma-H2AX dephosphorylation by protein phosphatase 2A facilitates DNA double-strand break repair. Mol Cell 2005; 20:801-9. (Pubitemid 41740701)
8. Chowdhury D, Xu X, Zhong X, Ahmed F, Zhong J, Liao J, et al. A PP4-phosphatase complex dephosphorylates gamma-H2AX generated during DNA replication. Mol Cell 2008; 31:33-46. (Pubitemid 351905923)
9. Keogh MC, Kim JA, Downey M, Fillingham J, Chowdhury D, Harrison JC, et al. A phosphatase complex that dephosphorylates gammaH2AX regulates DNA damage checkpoint recovery. Nature 2006; 439:497-501. (Pubitemid 43152843)
10. Nakada S, Chen GI, Gingras AC, Durocher D. PP4 is a gamma H2AX phosphatase required for recovery from the DNA damage checkpoint. EMBO Rep 2008; 9:1019-26.
11. Douglas P, Zhong J, Ye R, Moorhead GB, Xu X, Lees-Miller SP. Protein phosphatase 6 interacts with the DNA-dependent protein kinase catalytic subunit (DNA-PKcs) and dephosphorylates {gamma}-H2AX. Mol Cell Biol 2010; 30:1368-81.
12. Cha H, Lowe JM, Li H, Lee JS, Belova GI, Bulavin DV, et al. Wip1 directly dephosphorylates gamma- H2AX and attenuates the DNA damage response. Cancer Res 70:4112-22.
13. Macurek L, Lindqvist A, Voets O, Kool J, Vos HR, Medema RH. Wip1 phosphatase is associated with chromatin and dephosphorylates gammaH2AX to promote checkpoint inhibition. Oncogene 29:2281-91.
14. Moon SH, Lin L, Zhang X, Nguyen TA, Darlington Y, Waldman AS, et al. Wild-type p53-induced phosphatase 1 dephosphorylates histone variant gamma-H2AX and suppresses DNA double strand break repair. J Biol Chem 285:12935-47.
15. Moon SH, Nguyen TA, Darlington Y, Lu X, Donehower LA. Dephosphorylation of gammaH2AX by WIP1: An important homeostatic regulatory event in DNA repair and cell cycle control. Cell Cycle 2010; 9:2092-96.
16. Bastians H, Ponstingl H. The novel human protein serine/ threonine phosphatase 6 is a functional homologue of budding yeast Sit4p and fission yeast ppe1, which are involved in cell cycle regulation. J Cell Sci 1996; 109:2865-74. (Pubitemid 27029257)
17. Stefansson B, Brautigan DL. Protein phosphatase 6 subunit with conserved Sit4-associated protein domain targets IkappaBepsilon. J Biol Chem 2006; 281:22624-34.
18. Stefansson B, Ohama T, Daugherty AE, Brautigan DL. Protein phosphatase 6 regulatory subunits composed of ankyrin repeat domains. Biochemistry 2008; 47:1442-51. (Pubitemid 351198719)
19. Filali M, Li S, Kim HW, Wadzinski B, Kamoun M. Identification of a type 6 protein ser/thr phosphatase regulated by interleukin-2 stimulation. J Cell Biochem 1999; 73:153-63. (Pubitemid 29160183)
20. Stefansson B, Brautigan DL. Protein phosphatase PP6 N terminal domain restricts G1 to S phase progression in human cancer cells. Cell Cycle 2007; 6:1386-92. (Pubitemid 47327974)
21. Mi J, Dziegielewski J, Bolesta E, Brautigan DL, Larner JM. Activation of DNA-PK by ionizing radiation is mediated by protein phosphatase 6. PLoS One 2009; 4:4395.
22. Chan DW, Chen BP, Prithivirajsingh S, Kurimasa A, Story MD, Qin J, et al. Autophosphorylation of the DNA-dependent protein kinase catalytic subunit is required for rejoining of DNA double-strand breaks. Genes Dev 2002; 16:2333-8. (Pubitemid 35034402)
23. Kurimasa A, Kumano S, Boubnov NV, Story MD, Tung CS, Peterson SR, et al. Requirement for the kinase activity of human DNA-dependent protein kinase catalytic subunit in DNA strand break rejoining. Mol Cell Biol 1999; 19:3877-84. (Pubitemid 29193847)
24. Jeggo P, Lavin MF. Cellular radiosensitivity: How much better do we understand it? Int J Radiat Biol 2009; 85:1061-81.
25. Ben-Yehoyada M, Gautier J, Dupre A. The DNA damage response during an unperturbed S-phase. DNA Repair (Amst) 2007; 6:914-22. (Pubitemid 46880472)
26. Pommier Y. Camptothecins and topoisomerase I: A foot in the door. Targeting the genome beyond topoisomerase I with camptothecins and novel anticancer drugs: Importance of DNA replication, repair and cell cycle checkpoints. Curr Med Chem Anticancer Agents 2004; 4:429-34.
27. Hochegger H, Sonoda E, Takeda S. Post-replication repair in DT40 cells: Translesion polymerases versus recombinases. Bioessays 2004; 26:151-8. (Pubitemid 38197013)
28. Pierce AJ, Johnson RD, Thompson LH, Jasin M. XRCC3 promotes homology-directed repair of DNA damage in mammalian cells. Genes Dev 1999; 13:2633-8. (Pubitemid 29508550)
29. Weinstock DM, Nakanishi K, Helgadottir HR, Jasin M. Assaying double-strand break repair pathway choice in mammalian cells using a targeted endonuclease or the RAG recombinase. Methods Enzymol 2006; 409:524-40.
30. Ali A, Zhang J, Bao S, Liu I, Otterness D, Dean NM, et al. Requirement of protein phosphatase 5 in DNAdamage- induced ATM activation. Genes Dev 2004; 18:249-54. (Pubitemid 38199250)
31. Zhang J, Bao S, Furumai R, Kucera KS, Ali A, Dean NM, et al. Protein phosphatase 5 is required for ATRmediated checkpoint activation. Mol Cell Biol 2005; 25:9910-9. (Pubitemid 41572739)
32. Wang H, Zhao A, Chen L, Zhong X, Liao J, Gao M, et al. Human RIF1 encodes an anti-apoptotic factor required for DNA repair. Carcinogenesis 2009; 30:1314-9.