Gradually accumulating beneficial mutations to improve the thermostability of N-carbamoyl-d-amino acid amidohydrolase by step-wise evolution

Applied Microbiology and Biotechnology

Volumn 90, Issue 4, 2011, Pages 1361-1371

  Zhang, Dalong ^a,b   Zhu, Fuyun ^c   Fan, Wenchao ^c   Tao, Rongsheng ^c   Yu, Hong ^a   Yang, Yunliu ^a   Jiang, Weihong ^a   Yang, Sheng ^a,c,d  

^a INSTITUTE OF PLANT PHYSIOLOGY AND ECOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c Chinese Academy of Sciences   (China)

^d Shanghai Research and Development Center of Industrial Biotechnology   (China)

Author keywords

Consensus concept; d p Hydroxyphenylglycine; Directed evolution; N carbamoyl d amino acid amidohydrolase; Thermostability

Indexed keywords

CARBAMOYL; CONSENSUS CONCEPT; D-P-HYDROXYPHENYLGLYCINE; DIRECTED EVOLUTION; THERMOSTABILITY;

ENZYMES; HEAT RESISTANCE; HYDROGEN PEROXIDE; ISOMERS; POLYMERASE CHAIN REACTION;

AMINO ACIDS;

BACTERIAL ENZYME; DEXTRO AMINO ACID; HYDROGEN PEROXIDE; HYDROLASE; MUTANT PROTEIN; N CARBAMOYL DEXTRO AMINO ACID AMIDOHYDROLASE; UNCLASSIFIED DRUG;

AMINO ACID; ASSAY; BIOTECHNOLOGY; COMPARATIVE STUDY; ENZYME ACTIVITY; HEATING; HYDROGEN PEROXIDE; INDUSTRIAL PRODUCTION; MUTATION; PHENOLIC COMPOUND; POLYMERASE CHAIN REACTION; REACTION KINETICS; SOLUBILITY; THERMODYNAMIC PROPERTY;

AMINO ACID SEQUENCE; ARTICLE; DNA SHUFFLING; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME STABILITY; GENE MUTATION; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; POLYMERASE CHAIN REACTION; RALSTONIA PICKETTII; SEQUENCE ALIGNMENT; THERMOSTABILITY;

AMIDOHYDROLASES; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; ENZYME STABILITY; HOT TEMPERATURE; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; RALSTONIA PICKETTII;

RALSTONIA PICKETTII;

EID: 79955559293     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-011-3114-9     Document Type: Article

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