Improving the thermostability of N-carbamyl-d-amino acid amidohydrolase by error-prone PCR

Applied Microbiology and Biotechnology

Volumn 82, Issue 2, 2009, Pages 279-285

  Yu, Hong ^a,b   Li, Jian ^a,b   Zhang, Dalong ^a,b   Yang, Yunliu ^a   Jiang, Weihong ^a   Yang, Sheng ^a,c  

^a Chinese Academy of Sciences   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c Shanghai Research and Development Center of Industrial Biotechnology   (China)

Author keywords

D amino acid; Error prone PCR; N carbamyl d amino acid amidohydrolase; Thermostability

Indexed keywords

ACID SITES; D-AMINO ACID; ENZYMATIC PROCESS; ERROR-PRONE; ERROR-PRONE PCR; HIGH SOLUBILITIES; INDUSTRIAL PRODUCTIONS; KINETIC PROPERTIES; MUTATED ENZYMES; MUTATION SITES; N-CARBAMYL-D-AMINO ACID AMIDOHYDROLASE; OXIDATIVE STABILITIES; RALSTONIA PICKETTII; THERMOSTABILITY;

AMINATION; AMINES; COSMIC RAYS; ISOMERS; ORGANIC ACIDS;

AMINO ACIDS;

AMINO ACID; BACTERIUM; ENZYME ACTIVITY; MUTATION; POLYMERASE CHAIN REACTION; REACTION KINETICS; TEMPERATURE TOLERANCE;

AMIDOHYDROLASES; BACTERIAL PROTEINS; DNA SHUFFLING; ENZYME STABILITY; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; POLYMERASE CHAIN REACTION; PROTEIN ENGINEERING; RALSTONIA PICKETTII; TEMPERATURE;

RALSTONIA PICKETTII;

EID: 59449102493     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1748-z     Document Type: Article

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