Effects of adeno-associated virus-2-mediated human BMP-7 gene transfection on the phenotype of nucleus pulposus cells

Journal of Orthopaedic Research

Volumn 29, Issue 6, 2011, Pages 838-845

  Wang, Chaofeng ^a   Ruan, Di Ke ^a   Zhang, Chao ^a   Wang, De Li ^a   Xin, Hongkui ^a   Zhang, Yan ^a  

^a NAVY GENERAL HOSPITAL   (China)

Author keywords

adeno associated virus; bone morphogenetic protein 7; gene therapy; nucleus pulposus

Indexed keywords

AGGRECAN; COLLAGEN TYPE 1; COLLAGEN TYPE 2; OSTEOGENIC PROTEIN 1; PROTEOGLYCAN;

ADENO ASSOCIATED VIRUS; ANIMAL CELL; ANIMAL CELL CULTURE; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CELL PROLIFERATION; CELL SECRETION; CLINICAL ASSESSMENT; CONTROLLED STUDY; DOG; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; GENE THERAPY; GENE VECTOR; GENETIC TRANSFECTION; HUMAN; INTERVERTEBRAL DISK DEGENERATION; MALE; NONHUMAN; NUCLEUS PULPOSUS; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

AGGRECANS; ANIMALS; BONE MORPHOGENETIC PROTEIN 7; CELL PROLIFERATION; COLLAGEN TYPE I; COLLAGEN TYPE II; DEPENDOVIRUS; DOGS; GENE EXPRESSION; GENE THERAPY; GENETIC VECTORS; HUMANS; INTERVERTEBRAL DISK; PHENOTYPE; PROTEOGLYCANS; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TRANSFECTION;

EID: 79955484053     PISSN: 07360266     EISSN: 1554527X     Source Type: Journal    
DOI: 10.1002/jor.21310     Document Type: Article

References (42)

1. Hiraki Y, Inoue H, Kato Y,. et al. 1987. Combined effects of somatomedin-like growth factors with fibroblast growth factor or epidermal growth factor in DNA synthesis in rabbit chondrocytes. Mol Cell Bio Chem 76: 185-193. (Pubitemid 17128034)
2. Zhang R, Ruan D, Zhang C,. et al. 2008. Research on biological characteristics of human chondrocyte-like cells in nucleus pulposus. Chin J Spine Spinal Cord 18 (11): 870-874.
3. Asahina I, Sampath TK, Nishimura I, et al. 1993. Human osteogenic protein-1 induces both chondroblastic and osteoblastic differentiation of osteoprogenitor cells derived from newborn rat calvaria. J Cell Biol 123: 921-933. (Pubitemid 23333580)
4. Asahina I, Sampath TK, Hauschka PV,. 1996. Human osteogenic protein-1 induces chondroblastic, osteoblastic, and/or adipocytic differentiation of clonal murine target cells. Exp Cell Res 222: 38-47. (Pubitemid 26043710)
5. Chen P, Vukicevic S, Sampath TK, et al. 1993. Bovine articular chondrocytes do not undergo hypertrophy when cultured in the presence of serum and osteogenic protein-1. Biochem Biophys Res Commun 197: 1253-1259. (Pubitemid 24042757)
6. Flechtenmacher J, Huch K, Thonar EJ, et al. 1996. Recombinant human osteogenic protein 1 is a potent stimulator of the synthesis of cartilage proteoglycans and collagens by human articular chondrocytes. Arthritis Rheum 39: 1896-1904. (Pubitemid 26378582)
7. An HS, Takegami K, Kamada H, et al. 2005. Intradiscal administration of osteogenic protein-1 increases intervertebral disc height and proteoglycan content in the nucleus pulposus in normal adolescent rabbits. Spine 30: 25-31. (Pubitemid 40209272)
8. Masuda K, Imai Y, Okuma M, et al. 2006. Osteogenic protein-1 injection into a degenerated disc induces the restoration of disc height and structural changes in the rabbit anular puncture model. Spine 31: 742-754.
9. Zhang Y, Phillips FM, Thonar EJ, et al. 2008. Cell therapy using articular chondrocytes overexpressing BMP-7 or BMP-10 in a rabbit disc organ culture model. Spine 15 (338): 831-838. (Pubitemid 351533849)
10. Zhang Y, Markova D, Im HJ, et al. 2009. Primary bovine intervertebral disc cells transduced with adenovirus overexpressing 12 BMPs and Sox9 maintain appropriate phenotype. Am J Phys Med Rehabil 88 (6): 455-463.
11. Zhang Y, An HS, Thonar EJ, et al. 2006. Comparative effects of bone morphogenetic proteins and sox9 overexpression on extracellular matrix metabolism of bovine nucleus pulposus cells. Spine 31 (19): 2173-2179. (Pubitemid 44337852)
12. Douglas JT,. 2007. Adenoviral vectors for gene therapy. Mol Biotechnol 36 (1): 71-80. (Pubitemid 350133603)
13. Schwarz EM,. 2000. The adeno-associated virus vector for orthopaedic gene therapy. Clin Orthop 379: S31-S39.
14. Choi VW, Asokan A, Haberman RA, et al. 2007. Production of recombinant adeno-associated viral vectors for in vitro and in vivo use. Curr Protoc Mol Biol 16: 25.
15. Madry H, Cucchiarini M, Terwilliger EF, et al. 2003. Recombinant adeno-associated virus vectors efficiently and persistently transduce chondrocytes in normal and osteoarthritic human articular cartilage. Hum Gene Ther 14 (4): 393-402. (Pubitemid 36308358)
16. During MJ, Samulski RJ, Elsworth JD, et al. 1998. In vivo expression of therapeutic human genes for dopamine production in the caudates of MPTP-treated monkeys using an AAV vector. Gene Ther 5 (6): 820-827.
17. McCown TJ, Xiao X, Li J, et al. 1996. Differential and persistent expression patterns of CNS gene transfer by an adeno-associated virus (AAV) vector. Brain Res 713 (1-2): 99-107. (Pubitemid 26114833)
18. Koeberl DD, Alexander IE, Halbert CL, et al. 1997. Persistent expression of human clotting factor IX from mouse liver after intravenous injection of adeno-associated virus vectors. Proc Natl Acad Sci USA 94 (4): 1426-1431. (Pubitemid 27087867)
19. Wang L, Nichols TC, Read MS, et al. 2000. Sustained expression of therapeutic level of factor IX in hemophilia B dogs by AAV-mediated gene therapy in liver. Mol Ther 1 (2): 154-158.
20. Elena I, Pan ZH,. 2009. Evaluation of the adeno-associated virus mediated long-term expression of channelrhodopsin-2 in the mouse retina. Mol Vis 15: 1680-1689.
21. Chelberg MK, Banks GM, Geiger DF,. et al. 1995. Identification of heterogenous cell populations in normal human intervertebral disc. J Anat 186: 43-53.
22. Tiemann C, Vogel A, Dufaux B, et al. 2001. Rapid DNA typing of HLA-B27 allele by real-time PCR using lightcycler technology. Clin Lab 47: 131-134. (Pubitemid 32236959)
23. Yin JL, Shackel NA, Zekry A, et al. 2001. Real-time reverse transcriptase polymerase chain reaction (RT-PCR) for measurement of cytokine and growth factor mRNA expression with fluorogenic probes or SYBR Green I. Immunol Cell Biol 79: 213-221. (Pubitemid 32522168)
24. Goldberg RL, Kolibas LM,. 1990. An improved method for determining proteoglycans synthesized by chondrocytes in culture. Connect Tissue Res 24: 265-275.
25. Yoon ST,. 2005. Molecular therapy of the intervertebral disc. Spine J 5: S280-S286.
26. Kim DJ, Moon SH, Kim H, et al. 2003. Bone morphogenetic protein-2 facilitates expression of chondrogenic, not osteogenic, phenotype of human intervertebral disc cells. Spine 28: 2679-2684. (Pubitemid 37543473)
27. Zhang Y, An HS, Song S, et al. 2004. Growth factor osteogenic protein-1: differing effects on cells from three distinct zones in the bovine intervertebral disc. Am J Phys Med Rehabil 83: 515-521. (Pubitemid 38802321)
28. Paul R, Haydon RC, Cheng H, et al. 2003. Potential use of Sox9 gene therapy for intervertebral degenerative disc disease. Spine 28: 755-763. (Pubitemid 36459290)
29. Yoon ST, Park JS, Kim KS, et al. 2004. ISSLS prize winner: LMP-1 upregulates intervertebral disc cell production of proteoglycans and BMPs in vitro and in vivo. Spine 29: 2603-2611. (Pubitemid 40353245)
30. Masuda K, Takegami K, An H, et al. 2003. Recombinant osteogenic protein-1 upregulates extracellular matrix metabolism by rabbit annulus fibrosus and nucleus pulposus cells cultured in alginate beads. J Orthop Res 21: 922-930. (Pubitemid 37045572)
31. Takegami K, Thonar EJ, An HS, et al. 2002. Osteogenic protein-1 enhances matrix replenishment by intervertebral disc cells previously exposed to interleukin-1. Spine 27: 1318-1325.
32. Flotte TR, Zeitlin PL, Reynolds TC, et al. 2003. Phase I trial of intranasal and endobronchial administration of a recombinant adeno-associated virus serotype 2 (rAAV2)-CFTR vector in adult cystic fibrosis patients: a two-part clinical study. Hum Gene Ther 14 (11): 1079-1088. (Pubitemid 36851399)
33. Wagner JA, Nepomuceno IB, Messner AH, et al. 2002. A phase II, doubleblind, randomized, placebo-controlled clinical trial of tgAAVCF using maxillary sinus delivery in patients with cystic fibrosis with antrostomies. Hum Gene Ther 13 (11): 1349-1359. (Pubitemid 35034514)
34. Aitken ML, Moss RB, Waltz DA, et al. 2001. A phase I study of aerosolized administration of tgAAVCF to cystic fibrosis subjects with mild lung disease. Hum Gene Ther 12 (15): 1907-1916. (Pubitemid 32947233)
35. Cappello R, Bird JL, Pfeiffer D,. et al. 2006. Notochordal cell produce and assemble extracellular matrix in a distinct manner, which may be responsible for the maintenance of healthy nucleus pulposus. Spine 31 (8): 873-882.
36. Mark W, Robert D,. 2006. Notochord cells regulate intervertebral disc chondrocyte proteoglycan production and cell proliferation. Spine 31 (10): 1094-1099.
37. Horwitz T,. 1977. The human notochord: a study of its development and regression, variations, and pathologic derivative, chordoma. Indianapolis: Horwitz.
38. Butler WF,. 1989. Comparative anatomy and development of the mammalian disc. In:, Gosh P, editor. The biology of the intervertebral disc. Boca Raton, FL: CRC Press; p 84-108.
39. Christopher JH, John RM, Neil AD, et al. 2004. Cytomorphology of notochordal and chondrocytic cells from the nucleus pulposus: a species comparison. J Anat 205 (5): 357-362.
40. Ganey T, Libera J, Moos V, et al. 2003. Disc chondrocyte transplantation in a canine model: a treatment for degenerated or damaged intervertebral disc. Spine 28: 2609-2620. (Pubitemid 37490470)
41. Hutton WC, Ganey TM, Elmer WA, et al. 2000. Does long-term compressive loading on the intervertebral disc cause degeneration? Spine 25: 2993-3004.
42. Qu FJ, Liu Y, Zhu DL,. 2008. Construction of tissue-engineered cartilage with BMP7 gene-transfected chondrocytes. J Int Med Res 36: 837-847.