Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) for measurement of cytokine and growth factor mRNA expression with fluorogenic probes or SYBR Green I

Immunology and Cell Biology

Volumn 79, Issue 3, 2001, Pages 213-221

  Yin, Jian Lin ^a   Shackel, Nicholas A ^a   Zekry, Amany ^a   McGuinness, Peter H ^a   Richards, Craig ^a   Van Der Putten, Karien ^a   McCaughan, Geoffrey W ^a   Eris, Josette M ^a   Bishop, G Alex ^a  

^a ROYAL PRINCE ALFRED HOSPITAL   (Australia)

Author keywords

Fluorescent resonance energy transfer (FRET) probe; Quantitative real time PCR; SYBR Green I; Taqman

Indexed keywords

COMPLEMENTARY DNA; CYTOKINE; DYE; GAMMA INTERFERON; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; GROWTH FACTOR; INTERLEUKIN 10; INTERLEUKIN 2; INTERLEUKIN 4; MESSENGER RNA; TRANSFORMING GROWTH FACTOR BETA; TUMOR NECROSIS FACTOR ALPHA;

ANIMAL CELL; ARTICLE; FLUORESCENCE; HUMAN; HUMAN TISSUE; KIDNEY BIOPSY; LIVER BIOPSY; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RAT; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANIMALS; BUFFERS; CYTOKINES; FLUORESCENT DYES; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; HUMANS; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; INTERFERON TYPE II; ORGANIC CHEMICALS; PROTEINS; RATS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SENSITIVITY AND SPECIFICITY;

ANIMALIA;

EID: 0034993341     PISSN: 08189641     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1440-1711.2001.01002.x     Document Type: Article

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