Purification of polyethylenimine polyplexes highlights the role of free polycations in gene transfer

Journal of Gene Medicine

Volumn 6, Issue 10, 2004, Pages 1102-1111

  Boeckle, Sabine ^a   von Gersdorff, Katharina ^a   van der Piepen, Silke ^a   Culmsee, Carsten ^a   Wagner, Ernst ^a   Ogris, Manfred ^a  

^a UNIVERSITY OF MUNICH   (Germany)

Author keywords

Endosomal release; Erythrocyte aggregation; Lung gene transfer; Nonviral vectors; PEI; Size exclusion chromatography

Indexed keywords

NITROGEN; PHOSPHATE; PLASMID DNA; POLYCATION; POLYETHYLENEIMINE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; COMPARATIVE STUDY; COMPLEX FORMATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY TEST; FEMALE; GEL PERMEATION CHROMATOGRAPHY; GENE EXPRESSION; GENE TRANSFER; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; METHODOLOGY; MOLECULAR MECHANICS; MOUSE; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; PRIORITY JOURNAL; PURIFICATION; STATISTICAL SIGNIFICANCE; ZETA POTENTIAL;

ANIMALS; CATIONS; CELL LINE; CHROMATOGRAPHY; DNA; ERYTHROCYTES; FEMALE; FLOW CYTOMETRY; GENE THERAPY; GENE TRANSFER TECHNIQUES; GENES, REPORTER; GENETIC TECHNIQUES; LASERS; MICE; MICE, INBRED BALB C; NITROGEN; PLASMIDS; POLYETHYLENEIMINE; TIME FACTORS; TISSUE DISTRIBUTION; TRANSFECTION;

EID: 11844290850     PISSN: 1099498X     EISSN: 15212254     Source Type: Journal    
DOI: 10.1002/jgm.598     Document Type: Article

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