Intracellular route and transcriptional competence of polyethylenimine-DNA complexes

Journal of Controlled Release

Volumn 82, Issue 2-3, 2002, Pages 441-454

  Bieber, Thorsten ^a   Meissner, Wolfgang ^a   Kostin, Sawa ^b   Niemann, Axel ^a   Elsasser, Hans Peter ^a  

^a PHILIPPS UNIVERSITY MARBURG   (Germany)

^b MAX PLANCK INSTITUTE FOR HEART AND LUNG RESEARCH   (Germany)

Author keywords

Confocal microscopy; Gene transfer; In vitro transcription; Lysosome; Polyethylenimine

Indexed keywords

POLYETHYLENEIMINE; RECOMBINANT DNA;

ARTICLE; CELL FRACTIONATION; CELL GRANULE; CELL NUCLEUS; DNA DRUG COMPLEX; DNA TEMPLATE; DNA TRANSCRIPTION; DRUG RELEASE; ELECTRON MICROSCOPY; FLUORESCENCE MICROSCOPY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IN VITRO STUDY; LYSOSOME; MEMBRANE DAMAGE; PRIORITY JOURNAL; CELL CULTURE; CHEMISTRY; CONFOCAL MICROSCOPY; CYTOPLASM; GENETIC TRANSCRIPTION; KIDNEY; METABOLISM; METHODOLOGY; PANCREAS TUMOR; PLASMID; PRENATAL DEVELOPMENT; TRANSPORT AT THE CELLULAR LEVEL; ULTRASTRUCTURE;

BIOLOGICAL TRANSPORT; CELL NUCLEUS; CYTOPLASM; DNA, RECOMBINANT; HUMANS; KIDNEY; LYSOSOMES; MICROSCOPY, CONFOCAL; MICROSCOPY, ELECTRON; PANCREATIC NEOPLASMS; PLASMIDS; POLYETHYLENEIMINE; TRANSCRIPTION, GENETIC; TRANSFECTION; TUMOR CELLS, CULTURED;

EID: 0037151312     PISSN: 01683659     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-3659(02)00129-3     Document Type: Article

References (25)

1. A powerful nonviral vector for in vivo gene transfer into the adult mammalian brain: Polyethylenimine
2. Polyethylenimine (PEI) is a simple, inexpensive and effective reagent for condensing and linking plasmid DNA to adenovirus for gene delivery
3. Optimized galenics improve in vitro gene transfer with cationic molecules up to 1000-fold
4. A novel non-viral vector for DNA delivery based on low molecular weight, branched polyethylenimine: Effect of molecular weight on transfection efficiency and cytotoxicity
5. Poly(ethylenimine)-mediated transfection: A new paradigm for gene delivery
6. Nonviral gene delivery to the rat kidney with polyethylenimine
7. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: Polyethylenimine
8. Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery
9. Coupling of cell-binding ligands to polyethylenimine for targeted gene delivery
10. Uptake and intracellular fate of polyethylenimine in vivo
11. Effects of polyethyleneimine on endocytosis and lysosome stability
12. Polyethylenimine but not cationic lipids promotes transgene delivery to the nucleus in mammalian cells
13. Monodansylcadaverine (MDC) is a specific in vivo marker for autophagic vacuoles
14. The proton sponge: A trick to enter cells viruses did not exploit
15. Transcription complexes for various class III genes differ in parameters of formation and stability towards salt
16. Human transcription factors IIIC2, IIIC1 and a novel component IIIC0 fulfil different aspects of DNA binding to various pol III genes
17. Purification of human transcription factor IIIA and its interaction with a chemically synthesized gene encoding human 5 S rRNA
18. Preparation of a low molecular weight polyethylenimine for efficient cell transfection
19. Purification of human transcription factor IIIC and its binding to the gene for ribosomal 5S RNA
20. Structural analysis of a new highly metastatic cell line PaTu 8902 from a primary human pancreatic adenocarcinoma
21. Spatiotemporal development and distribution of intercellular junctions in adult rat cardiomyocytes in culture
22. Tissue-specific patterns of gap junctions in adult rat atrial and ventricular cardiomyocytes in vivo and in vitro
23. Formaldehyde-glutaraldehyde fixatives containing trinitro compounds
24. Endosomes, lysosomes: Their implication in gene transfer
25. Intracellular processing of poly(etyleneimine)/ribozyme complexes can be observed in living cells by using confocal laser scanning microscopy and inhibitor experiments