Monitoring of the formation and dissociation of polyethylenimine/DNA complexes by two photon fluorescence correlation spectroscopy

Biophysical Journal

Volumn 84, Issue 3, 2003, Pages 1960-1968

  Clamme, Jean Pierre ^a   Azoulay, Joel ^a   Mély, Yves ^a  

^a UNIVERSITÉ DE STRASBOURG   (France)

Author keywords

[No Author keywords available]

Indexed keywords

HEPARIN; NITROGEN; PHOSPHATE; PLASMID DNA; POLYETHYLENEIMINE; RHODAMINE;

ARTICLE; COMPLEX FORMATION; DISSOCIATION; FLUORESCENCE SPECTROSCOPY; GENE THERAPY; GENETIC TRANSFECTION; NONVIRAL GENE DELIVERY SYSTEM; PKA; QUANTITATIVE ANALYSIS; STOICHIOMETRY; TWO PHOTON FLUORESCENCE CORRELATION SPECTROSCOPY; ULTRAFILTRATION;

EID: 0037340561     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(03)75004-8     Document Type: Article

References (47)

1. Andersson, M. M., R. Hatti-Kaul, and W. Brown. 2000. Dynamic and static light scattering and fluorescence studies of the interactions between lactate dehydrogenase and poly(ethyleneimine). J. Phys. Chem. B. 104:3660-3667.
2. Bernacchi, S., and Y. Mely. 2001. Exciton interaction in molecular beacons: a sensitive sensor for short range modifications of the nucleic acid structure. Nucleic Acids Res. 29:E62-2.
3. Blessing, T., M. Kursa, R. Holzhauser, R. Kircheis, and E. Wagner. 2001. Different strategies for formation of pegylated EGF-conjugated PEI/DNA complexes for targeted gene delivery. Bioconjug. Chem. 12:529-537.
4. Blessing, T., J. S. Remy, and J. P. Behr. 1998. Template oligomerization of DNA-bound cations produces calibrated nanometric particles. J. Am. Chem. Soc. 120:8519-8520.
5. Bloomfield, V. A. 1991. Condensation of DNA by multivalent cations: considerations on mechanism. Biopolymers. 31:1471-1481.
6. Bloomfield, V. A., D. M. Crothers, and I. Tinoco. 2000. Nucleic Acids: Structures, Properties, and Functions. University Science Books, Sausalito, CA.
7. Boussif, O., F. Lezoualc'h, M. A. Zanta, M. D. Mergny, D. Scherman, B. Demeneix, and J. P. Behr. 1995. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine. Proc. Natl. Acad. Sci. USA. 92:7297-7301.
8. Cantor, C., and P. Schimmel. 1980. Techniques for the study of biological structure and function. W.H Freeman, San Francisco.
9. Dunlap, D. D., A. Maggi, M. R. Soria, and L. Monaco. 1997. Nanoscopic structure of DNA condensed for gene delivery. Nucleic Acids Res. 25:3095-3101.
10. Erbacher, P., T. Bettinger, P. Belguise-Valladier, S. Zou, J. L. Coll, J. P. Behr, and J. S. Remy. 1999. Transfection and physical properties of various saccharide, poly(ethylene glycol), and antibody-derivatized polyethylenimines (PEI). J. Gene Med. 1:210-222.
11. Finsinger, D., J. S. Remy, P. Erbacher, C. Koch, and C. Plank. 2000. Protective copolymers for nonviral gene vectors: synthesis, vector characterization and application in gene delivery. Gene Ther. 7:1183-1192.
12. Fischer, D., T. Bieber, Y. Li, H. P. Elsasser, and T. Kissel. 1999. A novel non-viral vector for DNA delivery based on low molecular weight, branched polyethylenimine: effect of molecular weight on transfection efficiency and cytotoxicity. Pharm. Res. 16:1273-1279.
13. Godbey, W. T., M. A. Barry, P. Saggau, K. K. Wu, and A. G. Mikos. 2000. Poly(ethylenimine)-mediated transfection: a new paradigm for gene delivery. J. Biomed. Mater. Res. 51:321-328.
14. Godbey, W. T., and A. G. Mikos. 2001. Recent progress in gene delivery using non-viral transfer complexes. J Control Release. 72:115-125.
15. Godbey, W. T., K. K. Wu, G. J. Hirasaki, and A. G. Mikos. 1999a. Improved packing of poly(ethylenimine)/DNA complexes increases transfection efficiency. Gene Ther. 6:1380-1388.
16. Godbey, W. T., K. K. Wu, and A. G. Mikos. 1999b. Poly(ethylenimine) and its role in gene delivery. J Control Release. 60:149-160.
17. Godbey, W. T., K. K. Wu, and A. G. Mikos. 1999c. Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery. Proc. Natl. Acad. Sci. USA. 96:5177-5181.
18. Godbey, W. T., K. K. Wu, and A. G. Mikos. 2001. Poly(ethylenimine)-mediated gene delivery affects endothelial cell function and viability. Biomaterials. 22:471-480.
19. Helander, I. M., H. L. Alakomi, K. Latva-Kala, and P. Koski. 1997. Polyethyleneimine is an effective permeabilizer of gram-negative bacteria. Microbiology. 143:3193-3199.
20. Helander, I. M., K. Latva-Kala, and K. Lounatmaa. 1998. Permeabilizing action of polyethyleneimine on Salmonella typhimurium involves disruption of the outer membrane and interactions with lipopolysaccharide. Microbiology. 144:385-390.
21. Hellweg, T., N. Henry-Toulme, M. Chambon, and D. Roux. 2000. Interaction of short DNA fragments with the cationic polyelectrolyte poly(ethyleneimine): a dynamic light scattering study. Colloid Surf. A-Physicochem. Eng. Asp. 163:71-80.
22. Hess, S. T., S. H. Huang, A. A. Heikal, and W. W. Webb. 2002. Biological and chemical applications of fluorescence correlation spectroscopy: a review. Biochemistry. 41:697-705.
23. Kichler, A., P. Erbacher, and J. P. Behr. 1999. Polyethylenimines: a family of potent polymers for nucleic acid delivery. In Non-viral Vectors for Gene Therapy. L. Huang, M. Hung, and E. Wagner, editors. Academic Press, San Diego. 190-206.
24. Klemm, A. R., D. Young, and J. B. Lloyd. 1998. Effects of polyethyleneimine on endocytosis and lysosome stability. Biochem. Pharmacol. 56:41-46.
25. Kral, T., M. Hof, and M. Langner. 2002a. The effect of spermine on plasmid condensation and dye release observed by fluorescence correlation spectroscopy. Biol. Chem. 383:331-335.
26. Kral, T., M. Langner, M. Benes, D. Baczynska, M. Ugorski, and M. Hof. 2002b. The application of fluorescence correlation spectroscopy in detecting DNA condensation. Biophys. Chem. 95:135-144.
27. Livesey, A. K., and J. C. Brochon. 1987. Analyzing the distribution of decay constants in pulse-fluorimetry using the maximum entropy method. Biophys. J. 52:693-706.
28. Meseth, U., T. Wohland, R. Rigler, and H. Vogel. 1999. Resolution of fluorescence correlation measurements. Biophys. J. 76:1619-1631.
29. Moret, I., J. Esteban Peris, V. M. Guillem, M. Benet, F. Revert, F. Dasi, A. Crespo, and S. F. Alino. 2001. Stability of PEI-DNA and DOTAP-DNA complexes: effect of alkaline pH, heparin and serum. J. Control Release. 76:169-181.
30. Ogris, M., P. Steinlein, M. Kursa, K. Mechtler, R. Kircheis, and E. Wagner. 1998. The size of DNA/transferrin-PEI complexes is an important factor for gene expression in cultured cells. Gene Ther. 5:1425-1433.
31. Oku, N., N. Yamaguchi, S. Shibamoto, F. Ito, and M. Nango. 1986. The fusogenic effect of synthetic polycations on negatively charged lipid bilayers. J. Biochem. (Tokyo). 100:935-944.
32. Packard, B. Z., D. D. Toptygin, A. Komoriya, and L. Brand. 1996. Profluorescent protease substrates: intramolecular dimers described by the exciton model. Proc. Natl. Acad. Sci. USA. 93:11640-11645.
33. Putnam, D., and R. Langer. 1999. Poly(4-hydroxy-L-proline ester): low-temperature polycondensation and plasmid DNA complexation. Macromolecules. 32:3658-3662.
34. Remy, J. S., D. Goula, A. M. Steffan, M. A. Zanta, O. Boussif, J. P. Behr, and B. Demeneix. 1998. Taking lipospermines and polyethylenimine from in vitro to in vivo. In Self-Assembling Complexes for Gene Delivery; From Laboratory to Clinical Trial. A. Kabanov, L. W. Seymour, P. Felgner, editors. Wiley & Sons, Chichester. 135-148.
35. Remy-Kristensen, A., J. P. Clamme, C. Vuilleumier, J. G. Kuhry, and Y. Mely. 2001. Role of endocytosis in the transfection of L929 fibroblasts by polyethylenimine/DNA complexes. Biochim. Biophys. Acta. 1514:21-32.
36. Rigler, R., Ü. Mets, J. Widengren, and P. Kask. 1993. Fluorescence correlation spectroscopy with high count rate and low background. Eur. Biophys. J. 22:169-175.
37. Schwille, P. 2001. Fluorescence correlation spectroscopy and its potential for intracellular applications. Cell Biochem. Biophys. 34:383-408.
38. Schwille, P., U. Haupts, S. Maiti, and W. W. Webb. 1999. Molecular dynamics in living cells observed by fluorescence correlation spectroscopy with one- and two-photon excitation. Biophys. J. 77:2251-2265.
39. Suh, J., S. H. Lee, S. M. Kim, and S. S. Hah. 1997. Conformational flexibility of poly(ethylenimine) and its derivatives. Bioorganic Chem. 25:221-231.
40. Suh, J., H. J. Paik, and B. K. Hwang. 1994. Ionization of poly(ethylenimine) and poly(allylamine) at various pHs. Bioorganic Chem. 22:312-327.
41. Swaminathan, T., and N. Periasamy. 1996. Analysis of fluorescence decay by the maximum entropy method: influence of noise and analysis parameters on the width of the distribution of lifetimes. Proc. Indian Acad. Sci. 108:39-49.
42. Tang, M. X., and F. C. Szoka. 1997. The influence of polymer structure on the interactions of cationic polymers with DNA and morphology of the resulting complexes. Gene Ther. 4:823-832.
43. Thompson, N. 1991. Fluorescence correlation spectroscopy. In Topics in Fluorescence Spectroscopy. R. Lakowicz, editor. Plenum Press, New York. 337-378.
44. Van Rompaey, E., Y. Engelborghs, N. Sanders, S. C. De Smedt, and J. Demeester. 2001. Interactions between oligonucleotides and cationic polymers investigated by fluorescence correlation spectroscopy. Pharm. Res. 18:928-936.
45. von Harpe, A., H. Petersen, Y. Li, and T. Kissel. 2000. Characterization of commercially available and synthesized polyethylenimines for gene delivery. J Control Release. 69:309-322.
46. Wang, M. M., G. L. Silva, and B. A. Armitage. 2000. DNA-templated formation of a helical cyanine dye J-aggregate. J. Am. Chem. Soc. 122:9977-9986.
47. Wilson, R. W., and V. A. Bloomfield. 1979. Counterion-induced condensation of deoxyribonucleic acid. A light-scattering study. Biochemistry. 18:2192-2196.