Detergent screening of a G-protein-coupled receptor using serial and array biosensor technologies

Analytical Biochemistry

Volumn 386, Issue 1, 2009, Pages 98-104

  Rich, Rebecca L ^a   Miles, Adam R ^b   Gale, Bruce K ^b,c   Myszka, David G ^a  

^a UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

^b Wasatch Microfluidics   (United States)

^c Department of Electrical and Computer Engineering   (United States)

Author keywords

Array; Biacore; CCR5; Flexchip; GPCR; Optical biosensor; Surface plasmon resonance

Indexed keywords

BIOSENSORS; PROTEINS; SURFACE PLASMON RESONANCE;

ARRAY; BIACORE; CCR5; FLEXCHIP; GPCR; OPTICAL BIO-SENSORS;

SOAPS (DETERGENTS);

CHEMOKINE RECEPTOR CCR5; DETERGENT; G PROTEIN COUPLED RECEPTOR;

ARTICLE; BIOASSAY; BIOSENSOR; CONFORMATION; CONTROLLED STUDY; MOLECULE; PRIORITY JOURNAL; PROTEIN BINDING; RECEPTOR SOLUBILIZATION; SCREENING;

EID: 58349120186     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.12.011     Document Type: Article

References (13)

1. Navratilova I., Sodroski J., and Myszka D.G. Solubilization, stabilization, and purification of chemokine receptors using Biacore technology. Anal. Biochem. 339 (2005) 271-281
2. Navratilova I., Pancera M., Wyatt R.T., and Myszka D.G. A biosensor-based approach toward purification and crystallization of G protein-coupled receptors. Anal. Biochem. 353 (2006) 278-283
3. Navratilova I., Myszka D.G., and Rich R.L. Probing membrane protein interactions with real-time biosensor technology. In: Pebay-Peyroula E. (Ed). Biophysical Analysis of Membrane Proteins (2007), Wiley-VCH, Weinheim, Germany 121-140
4. Mirzabekov T., Kontos H., Farzan M., Marasco W., and Sodroski J. Paramagnetic proteoliposomes containing a pure, native, and oriented seven-transmembrane segment protein, CCR5. Nat. Biotechnol. 18 (2000) 649-654
5. Rich R.L., and Myszka D.G. Higher throughput, label-free, real-time molecular interaction analysis. Anal. Biochem. 361 (2007) 1-6
6. Rich R.L., Cannon M.J., Jenkins J., Pandian P., Sundaram S., Magyar R., Brockman J., Lambert J., and Myszka D.G. Extracting kinetic rate constants from surface plasmon resonance array systems. Anal. Biochem. 373 (2008) 112-120
7. Navratilova I., Dioszegi M., and Myszka D.G. Analyzing ligand and small molecule binding activity of solubilized GPCRs using biosensor technology. Anal. Biochem. 355 (2006) 132-139
8. Chang-Yen D.A., Myszka D.G., and Gale B.K. A novel PDMS microfluidic spotter for fabrication of protein chips and microarrays. J. Microelectromech. Syst. 15 (2006) 1145-1151
9. Natarajan S., Katsamba P.S., Miles A., Eckman J., Papalia G.A., Rich R.L., Gale B., and Myszka D.G. Continuous flow microfluidic printing of proteins for array-based applications including surface plasmon resonance imaging. Anal. Biochem. 373 (2008) 141-146
10. 2 adrenergic G-protein-coupled receptor. Nature 450 (2007) 383-387
11. 2-adrenergic G protein coupled receptor. Science 318 (2007) 1258-1265
12. 2 adrenergic receptor function. Science 318 (2007) 1266-1273
13. Rich R.L., and Myszka D.G. Survey of the year 2003 commercial optical biosensor literature. J. Mol. Recognit. 18 (2005) 1-39