Single-cell ELISA and flow cytometry as methods for highlighting potential neuronal and astrocytic toxicant specificity

Neurotoxicity Research

Volumn 19, Issue 3, 2011, Pages 472-483

  Woehrling, E K ^a   Hill, E J ^a   Torr, E E ^a   Coleman, M D ^a  

^a ASTON UNIVERSITY   (United Kingdom)

Author keywords

Astrocytes; GFAP; Neurofilaments; Neurons; Neurotoxicity; NT2

Indexed keywords

ACRYLAMIDE; ATROPINE; CAFFEINE; CHLORAMPHENICOL; CHLOROQUINE; GLIAL FIBRILLARY ACIDIC PROTEIN; NEUROFILAMENT PROTEIN; NEUROTOXIN; NICOTINE; RIFAMPICIN; VERAPAMIL;

ARTICLE; ASTROCYTE; CANCER CELL CULTURE; CELL DEATH; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOSKELETON; CYTOTOXICITY; ENZYME LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; HEALTH HAZARD; HUMAN; HUMAN CELL; IC 50; NERVE CELL; NEUROTOXICITY; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ASTROCYTES; CELL LINE, TUMOR; CELL SURVIVAL; CYTOSKELETON; ENZYME-LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; GLIAL FIBRILLARY ACIDIC PROTEIN; HUMANS; NEURONS; NEUROTOXINS;

EID: 79951942317     PISSN: 10298428     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12640-010-9202-2     Document Type: Article

References (93)

1. Y Abreu-Villaca FJ Seidler D Qiao TA Slotkin 2005 Modeling the developmental neurotoxicity of nicotine in vitro: cell acquisition, growth and viability in PC12 cells Brain Res Dev Brain Res 154 239 246 1:CAS:528: DC%2BD2MXhtFOnu7o%3D 10.1016/j.devbrainres.2004.10.018 15707677 (Pubitemid 40221868)
2. ECV AM 2002 Target organ and target system toxicity Altern Lab Anim 30 1 71 82
3. Anderl JL, Redpath S, Ball AJ (2009) A neuronal and astrocyte co-culture assay for high content analysis of neurotoxicity. J Vis Exp. doi: 10.3791/1173
4. PW Andrews 1984 Retinoic acid induces neuronal differentiation of a cloned human embryonal carcinoma cell-line in vitro Dev Biol 103 285 293 1:CAS:528:DyaL2cXktlOntbo%3D 10.1016/0012-1606(84)90316-6 6144603
5. Anthony DC, Montine TJ, Valentine WM, Graham DG (2001) Chapter 16: toxic responses of the nervous system. In: Klassen CD (ed) Casarett and Doull's Toxicology: the basic science of poisons, 6th edn. McGraw-Hill, London, pp 535-564
6. A Araque ED Martin G Perea JI Arellano W Buno 2002 Synaptically released acetylcholine evokes Ca2+ elevations in astrocytes in hippocampal slices J Neurosci 22 2443 2450 1:CAS:528:DC%2BD38Xis1aqtrk%3D 11923408 (Pubitemid 37465792)
7. CK Atterwill A Bruinink J Drejer E Duarte EM Abdulla C Meredith, et al. 1994 In-vitro neurotoxicity testing-the report and recommendations of ECVAM workshop-3 ATLA Altern Lab Anim 22 350 362 (Pubitemid 2143850)
8. AK Bal-Price C Suñol DG Weiss, et al. 2008 Application of in vitro neurotoxicity testing for regulatory purposes: Symposium III summary and research needs Neurotoxicology 29 520 531 10.1016/j.neuro.2008.02.008 18417220
9. Bal-Price AK, Hogberg HT, Buzanska L, Coecke S (2009) Relevance of in vitro neurotoxicity testing for regulatory requirements: challenges to be considered. Neurotoxicol Teratol. doi: 10.1016/j.ntt.2008.12.003
10. M Bani-Yaghoub JM Felker CCG Naus 1999 Human NT2/D1 cells differentiate into functional astrocytes Neuroreport 10 3843 3846 1:STN:280: DC%2BD3c7nvV2ltg%3D%3D 10.1097/00001756-199912160-00022 10716220 (Pubitemid 30004843)
11. A Barbon I Vallini L La Via E Marchina 2003 Barlati S Glutamate receptor RNA editing: a molecular analysis of GluR2, GluR5 and GluR6 in human brain tissues and in NT2 cells following in vitro neural differentiation Mol. Brain Res 117 168 178 1:CAS:528:DC%2BD3sXotVymsLs%3D 10.1016/S0169-328X(03)00317-6 14559151 (Pubitemid 38366030)
12. LK Bechtel DM Haverstick CP Holstege 2008 Verapamil toxicity dysregulates the phosphatidylinositol 3-kinase pathway Acad Emerg Med 15 368 374 10.1111/j.1553-2712.2008.00088.x 18370992
13. AM Black S Pandya D Clark EA Armstrong JY Yager 2008 Effect of caffeine and morphine on the developing pre-mature brain Brain Res 1219 136 142 1:CAS:528:DC%2BD1cXnvVGgs7o%3D 10.1016/j.brainres.2008.04.066 18547548
14. A Bruinink G Zimmermann F Riesen 1991 Neurotoxic effects of chloroquine invitro Arch Toxicol 65 480 484 1:CAS:528:DyaK3MXlsV2rs7o%3D 10.1007/BF01977360 1929868
15. B Bunnemann A Terron V Zantedeschi EM Pich C Chiamulera 2000 Chronic nicotine treatment decreases neurofilament immunoreactivity in the rat ventral tegmental area Eur J Pharmacol 393 249 253 1:CAS:528:DC%2BD3cXisFKjs7g%3D 10.1016/S0014-2999(00)00104-7 10771021 (Pubitemid 30193424)
16. RL Carrier TC Ma K Obrietan KR Hoyt 2006 A sensitive and selective assay of neuronal degeneration in cell culture J Neurosci Meth 154 239 244 1:CAS:528:DC%2BD28Xltl2hsLw%3D 10.1016/j.jneumeth.2005.12.030 (Pubitemid 43816729)
17. O Cauli M Morelli 2005 Caffeine and the dopaminergic system Behav Pharmacol 16 63 77 1:CAS:528:DC%2BD2MXit1OntLc%3D 10.1097/00008877-200503000- 00001 15767841 (Pubitemid 40477532)
18. JH Chen KY Wu IM Chiu TC Tsou CC Chou 2009 Acrylamide-induced astrogliotic and apoptotic responses in human astrocytoma cells Toxicol In Vitro 23 855 861 1:CAS:528:DC%2BD1MXotFylsro%3D 10.1016/j.tiv.2009.04.015 19426797
19. RS Chung GH McCormack AE King AK West JC Vickers 2005 Glutamate induces rapid loss of axonal neurofilament proteins from cortical neurons in vitro Exp Neurol 193 481 488 1:CAS:528:DC%2BD2MXjslKju74%3D 10.1016/j.expneurol.2005.01. 005 15869950 (Pubitemid 40616195)
20. S Coecke C Eskes J Gartlon A Kinsner A Price E van Vliet, et al. 2006 The value of alternative testing for neurotoxicity in the context of regulatory needs Environ Toxicol Pharmacol 21 153 167 1:CAS:528:DC%2BD28XhtVGisbs%3D 10.1016/j.etap.2005.07.006 (Pubitemid 43163089)
21. MR Cookson VW Pentreath 1994 Alterations in the glial fibrillary acidic protein-content of primary astrocyte cultures for evaluation of glial-cell toxicity Toxicol In Vitro 8 351 356 1:CAS:528:DyaK2cXltVWntrc%3D 10.1016/0887-2333(94)90156-2 20692926 (Pubitemid 24219168)
22. MR Cookson NM Thatcher PG Ince PJ Shaw 1996 Selective loss of neurofilament proteins after exposure of differentiated human IMR-32 neuroblastoma cells to oxidative stress Brain Res 738 162 166 1:CAS:528:DyaK28Xms1aqurk%3D 10.1016/0006-8993(96)00992-4 8949942 (Pubitemid 26379660)
23. J DeJongh M Nordin-Andersson BA Ploeger A Forsby 1999 Estimation of systemic toxicity of acrylamide by integration of in vitro toxicity data with kinetic simulations Toxicol Appl Pharmacol 158 261 268 1:CAS:528: DyaK1MXkvFKis7k%3D 10.1006/taap.1999.8670 10438659 (Pubitemid 29382944)
24. S Desagher J Glowinski J Premont 1996 Astrocytes protect neurons from hydrogen peroxide toxicity J Neurosci 16 2553 2562 1:CAS:528:DyaK28XitFKhuro%3D 8786431 (Pubitemid 26122677)
25. HAN El-Fawal JP O'Callaghan 2008 Autoantibodies to neurotypic and gliotypic proteins as biomarkers of neurotoxicity: assessment of trimethyltin (TMT) Neurotoxicology 29 109 115 1:CAS:528:DC%2BD1cXlvVKrtw%3D%3D 10.1016/j.neuro.2007.09.009 18001836
26. LF Eng RS Ghirnikar YL Lee 2000 Glial fibrillary acidic protein: GFAP-thirty-one years (1969-2000) Neurochem Res 25 1439 1451 1:CAS:528:DC%2BD3cXns1eiuro%3D 10.1023/A:1007677003387 11059815
27. JH Exon 2006 A review of the toxicology of acrylamide J Toxicol Environ Health B Crit Rev 9 397 412 1:CAS:528:DC%2BD28Xht1GntLrN 10.1080/ 10937400600681430 17492525
28. A Forsby B Blaauboer 2007 Integration of in vitro neurotoxicity data with biokinetic modelling for the estimation of in vivo neurotoxicity Hum Exp Toxicol 26 333 338 1:CAS:528:DC%2BD2sXmt1CktrY%3D 10.1177/0960327106072994 17615114 (Pubitemid 46733356)
29. A Forsby AK Bal-Price A Camins S Coecke N Fabre H Gustafsson, et al. 2009 Neuronal in vitro models for the estimation of acute systemic toxicity Toxicol In Vitro 23 1564 1569 1:CAS:528:DC%2BD1MXhsVajtb%2FM 10.1016/j.tiv.2009.07.017 19615435
30. BB Fredholm 2007 Adenosine, an endogenous distress signal, modulates tissue damage and repair Cell Death Differ 14 1315 1323 1:CAS:528: DC%2BD2sXms1Srurk%3D 10.1038/sj.cdd.4402132 17396131 (Pubitemid 46965261)
31. J Gartlon A Kinsner A Bal-Price S Coecke RH Clothier 2006 Evaluation of a proposed in vitro test strategy using neuronal and non-neuronal cell systems for detecting neurotoxicity Toxicol In Vitro 20 1569 1581 1:CAS:528: DC%2BD28XhtVyit7rK 10.1016/j.tiv.2006.07.009 16959468 (Pubitemid 44537678)
32. ME Gegg B Beltran S Salas-Pino JP Bolanos JB Clark S Moncada, et al. 2003 Differential effect of nitric oxide on glutathione metabolism and mitochondrial function in astrocytes and neurons: implications for neuroprotection/ neurodegeneration? J Neurochem 86 228 237 1:CAS:528:DC%2BD3sXltFelu7s%3D 10.1046/j.1471-4159.2003.01821.x 12807442 (Pubitemid 36753863)
33. S Gelinas C Chapados M Beauregard I Gosselin MG Martinoli 2000 Effect of oxidative stress on stability and structure of neurofilament proteins Biochem Cell Biol 78 667 674 1:CAS:528:DC%2BD3MXlvFGhsw%3D%3D 10.1139/bcb-78-6-667 11206577 (Pubitemid 32102353)
34. ZZ Guan WF Yu A Nordberg 2003 Dual effects of nicotine on oxidative stress and neuroprotection in PC12 cells Neurochem Int 43 243 249 1:CAS:528:DC%2BD3sXis12hurk%3D 10.1016/S0197-0186(03)00009-3 12689604 (Pubitemid 36419525)
35. C Guerri M Pascual J Renau-Piqueras 2001 Glia and fetal alcohol syndrome Neurotoxicology 22 593 599 1:CAS:528:DC%2BD38XjvVaksA%3D%3D 10.1016/S0161- 813X(01)00037-7 11770880 (Pubitemid 33134234)
36. M Guizzetti T Moller LG Costa 2003 Ethanol inhibits muscarinic receptor-mediated DNA synthesis and signal transduction in human fetal astrocytes Neurosci Lett 344 68 70 1:CAS:528:DC%2BD3sXktFWlsb0%3D 10.1016/S0304-3940(03)00431-2 12781924 (Pubitemid 36627836)
37. J Haorah SH Ramirez N Floreani S Gorantla B Morsey Y Persidsky 2008 Mechanism of alcohol-induced oxidative stress and neuronal injury Free Radic Biol Med 45 1542 1550 1:CAS:528:DC%2BD1cXhsVSgtL3O 10.1016/j.freeradbiomed.2008. 08.030 18845238
38. GJ Harry M Billingsley A Bruinink IL Campbell W Classen DC Dorman, et al. 1998 In vitro techniques for the assessment of neurotoxicity Environ Health Perspect 106 131 158 1:CAS:528:DyaK1cXitlajs7o%3D 10.2307/3433917 9539010 (Pubitemid 28196041)
39. ZJ Herschman J Silverstein G Blumberg A Lehrfield 1991 Central-nervous-system toxicity from nebulized atropine sulfate J Toxicol Clin Toxicol 29 273 277 1:STN:280:DyaK3M3mvFertQ%3D%3D 10.3109/15563659109038621 2051513
40. LJ Holden MD Coleman 2008 Further preliminary assessment of three human glioma cell lines as models of human astrocytic toxicity in vitro Env Toxicol Pharmacol 26 290 296 1:CAS:528:DC%2BD1cXhtFOgtLvL 10.1016/j.etap.2008.05.009
41. SH Kang YA Lee SJ Won KH Rhee BJ Gwag 2002 Caffeine-induced neuronal death in neonatal rat brain and cortical cell cultures Neuroreport 13 1945 1950 1:CAS:528:DC%2BD38XotVClsb0%3D 10.1097/00001756-200210280-00023 12395097 (Pubitemid 35398966)
42. A Kinsner-Ovaskainen R Rzepka R Rudowski S Coecke T Cole P Prieto 2009 Acutoxbase, an innovative database for in vitro acute toxicity studies Toxicol In Vitro 23 476 485 1:CAS:528:DC%2BD1MXisFKlurc%3D 10.1016/j.tiv.2008.12.019 19159672
43. Kirkpatrick L, Brady S (1998) The cytoskeleton of neurons and glia, chap 11. In: Siegel GJ, Agranoff BW, Albers RW, Fisher SK, Uhler MD (eds) Basic neurochemistry: molecular, cellular and medical aspects, 6th edn. Lippincott, Williams and Wilkins, London, pp 135-174
44. A Knight 2008 Non-animal methodologies within biomedical research and toxicity testing Altex Altern Tierexp 25 213 231
45. CT Kong DE Holt SK Ma AKW Lie LC Chan 2000 Effects of antioxidants and a caspase inhibitor on chloramphenicol-induced toxicity of human bone marrow and HL-60 cells Hum Exp Toxicol 19 503 510 1:CAS:528:DC%2BD3MXnvFSltA%3D%3D 10.1191/096032700672635987 11204552 (Pubitemid 32111952)
46. RM Kostrzewa J Segura-Aguilar 2003 Novel mechanisms and approaches in the study of neurodegeneration and neuroprotection. A review Neurotox Res 5 375 383 10.1007/BF03033166 14715440
47. W Lilienblum W Dekant H Foth T Gebel JG Hengstler R Kahl, et al. 2008 Alternative methods to safety studies in experimental animals: role in the risk assessment of chemicals under the new European Chemicals Legislation (REACH) Arch Toxicol 82 211 236 1:CAS:528:DC%2BD1cXjvFKgt7c%3D 10.1007/s00204-008-0279-9 18322675
48. RM LoPachin M Aschner 1993 Glial neuronal interactions-relevance to neurotoxic mechanisms Toxicol Appl Pharmacol 118 141 158 1:CAS:528: DyaK3sXhvVGiuro%3D 10.1006/taap.1993.1020 8441994 (Pubitemid 23151336)
49. RM LoPachin T Gavin 2008 Acrylamide-induced nerve terminal damage: Relevance to neurotoxic and neurodegenerative mechanisms J Agric Food Chem 56 5994 6003 1:CAS:528:DC%2BD1cXosV2it7g%3D 10.1021/jf703745t 18624437
50. RM LoPachin AI Schwarcz CL Gaughan S Mansukhani S Das 2004 In vivo and in vitro effects of acrylamide on synaptosomal neurotransmitter uptake and release Neurotoxicology 25 349 363 1:CAS:528:DC%2BD2cXhslSktbk%3D 10.1016/S0161- 813X(03)00149-9 15019298 (Pubitemid 38283540)
51. F Monnet-Tschudi MG Zurich P Honegger 2007 Neurotoxicant-induced inflammatory response in three-dimensional brain cell cultures Hum Exp Toxicol 26 339 346 1:CAS:528:DC%2BD2sXmt1Cktrc%3D 10.1177/0960327107074589 17615115 (Pubitemid 46733357)
52. DJ Moore JK Chambers PR Murdock PC Emson 2002 Human Ntera-2/D1 neuronal progenitor cells endogenously express a functional P2Y1 receptor Neuropharmacology 43 966 978 1:CAS:528:DC%2BD38XotlyntLY%3D 10.1016/S0028- 3908(02)00177-6 12423666 (Pubitemid 35341233)
53. S Mottin P Laporte R Cespuglio 2003 Inhibition of NADH oxidation by chloramphenicol in the freely moving rat measured by picosecond time-resolved emission spectroscopy J Neurochem 84 633 642 1:CAS:528:DC%2BD3sXht1amsLY%3D 10.1046/j.1471-4159.2003.01508.x 12562508 (Pubitemid 36176922)
54. MB Newman YP Kuo RJ Lukas PR Sanberg RD Shytle MP McGrogan T Zigova 2002 Nicotinic acetylcholine receptors on NT2 precursor cells and hNT (NT2-N) neurons Dev Brain Res 139 73 86 1:CAS:528:DC%2BD38Xot1ajtrs%3D 10.1016/S0165-3806(02) 00513-8 (Pubitemid 35284682)
55. JP O'Callaghan K Sriram 2005 Glial fibrillary acidic protein and related glial proteins as biomarkers of neurotoxicity Expert Opin Drug Saf 4 433 442 10.1517/14740338.4.3.433 15934851 (Pubitemid 40723718)
56. JP O'Callaghan KF Jensen DB Miller 1995 Quantitative aspects of drug and toxicant-induced astrogliosis Neurochem Int 26 115 124 10.1016/0197-0186(94) 00106-5 7599532
57. TJ O'Shaughnessy B Zim W Ma KM Shaffer DA Stenger K Zamani, et al. 2003 Acute neuropharmacologic action of chloroquine on cortical neurons in vitro Brain Res 959 280 286 10.1016/S0006-8993(02)03763-0 12493616 (Pubitemid 35472190)
58. OECD Guideline for the testing of chemicals (1995) TG 418. Delayed neurotoxicity of organophosphorus substances following acute exposure, July 1995
59. OECD Guideline for the testing of chemicals (1997) TG 424. Neurotoxicity study in rodents, July 1997
60. OECD Guideline for the testing of chemicals (2007) TG 426. Developmental neurotoxicity study, October 2007
61. J Park D Kwon C Choi JW Oh EN Benveniste 2003 Chloroquine induces activation of nuclear factor-kappa B and subsequent expression of pro-inflammatory cytokines by human astroglial cells J Neurochem 84 1266 1274 1:CAS:528:DC%2BD3sXisVaqu7w%3D 10.1046/j.1471-4159.2003.01623.x 12614327 (Pubitemid 36343593)
62. JS Park KS Choi EJ Jeong DH Kwon EN Benveniste CH Choi 2004 Reactive oxygen species mediate chloroquine-induced expression of chemokines by human astroglial cells Glia 47 9 20 10.1002/glia.20017 15139008 (Pubitemid 38879701)
63. BC Park SH Park SH Paek SY Park MK Kwak HG Choi, et al. 2008 Chloroquine-induced nitric oxide increase and cell death is dependent on cellular GSH depletion in A172 human glioblastoma cells Toxicol Lett 178 52 60 1:CAS:528:DC%2BD1cXks12hsbY%3D 10.1016/j.toxlet.2008.02.003 18359172
64. D Qiao FJ Seidler TA Slotkin 2005 Oxidative mechanisms contributing to the developmental neurotoxicity of nicotine and chlorpyrifos Toxicol Appl Pharmacol 206 17 26 1:CAS:528:DC%2BD2MXlt1Gnsro%3D 10.1016/j.taap.2004.11.003 15963341 (Pubitemid 40800425)
65. E Robenshtok S Luria Z Tashma A Hourvitz 2002 Adverse reaction to atropine and the treatment of organophosphate intoxication Isr Med Assoc J 4 535 539 1:CAS:528:DC%2BD38XlvVKmsr4%3D 12120467 (Pubitemid 34752245)
66. JK Sandhu S Pandey M Ribecco-Lutkiewicz R Monette H Borowy-Borowski PR Walker, et al. 2003 Molecular mechanisms of glutamate neurotoxicity in mixed cultures of NT2-derived neurons and astrocytes: Protective effects of coenzyme Q(10) J Neurosci Res 72 691 703 1:CAS:528:DC%2BD3sXks1Gls7o%3D 10.1002/jnr.10579 12774309 (Pubitemid 36666262)
67. A Sbarbati B Bunnemann P Cristofori A Terron C Chiamulera F Merigo, et al. 2002 Chronic nicotine treatment changes the axonal distribution of 68 kDa neurofilaments in the rat ventral tegmental area Eur J Neurosci 16 877 882 10.1046/j.1460-9568.2002.02167.x 12372024 (Pubitemid 35463580)
68. G Schmuck R Kahl 2009 The use of Fluoro-Jade in primary neuronal cell cultures Arch Toxicol 83 397 403 1:CAS:528:DC%2BD1MXktVKgtbs%3D 10.1007/s00204-008-0360-4 18815771
69. G Schmuck HJ Ahr G Schluter 2000 Rat cortical neuron cultures: an in vitro model for differentiating mechanisms of chemically induced neurotoxicity In Vitro Mol Toxicol 13 37 49 1:CAS:528:DC%2BD3cXkvVynsL8%3D (Pubitemid 30457449)
70. J Segura-Aguilara RM Kostrzewa 2004 Neurotoxins and neurotoxic species implicated in neurodegeneration Neurotox Res 6 615 630 10.1007/BF03033456
71. S Sergent-Tanguy C Chagneau I Neveu P Naveilhan 2003 Fluorescent activated cell sorting (FACS): a rapid and reliable method to estimate the number of neurons in a mixed population J Neurosci Meth 129 73 79 10.1016/S0165-0270(03)00210-3 (Pubitemid 37013667)
72. MK Shelton KD McCarthy 2000 Hippocampal astrocytes exhibit Ca2+-elevating muscarinic cholinergic and histaminergic receptors in situ J Neurochem 74 555 563 1:CAS:528:DC%2BD3cXntFOqsA%3D%3D 10.1046/j.1471-4159.2000.740555.x 10646506 (Pubitemid 30053727)
73. C Shen XD Cheng DH Li Q Meng 2009 Investigation of rifampicin-induced hepatotoxicity in rat hepatocytes maintained in gel entrapment culture Cell Biol Toxicol 25 265 274 1:CAS:528:DC%2BD1MXltl2gt7o%3D 10.1007/s10565-008-9076-8 18496758
74. FD Shi WF Piao YP Kuo DI Campagnolo TL Vollmer RJ Lukas 2009 Nicotinic attenuation of central nervous system inflammation and autoimmunity J Immunol 182 1730 1739 1:CAS:528:DC%2BD1MXnsVCisw%3D%3D 10.4049/jimmunol.0803838 19155522
75. K Tieu PC Ashe DM Zuo PH Yu 2001 Inhibition of 6-hydroxydopamine-induced p53 expression and survival of neuroblastoma cells following interaction with astrocytes Neuroscience 103 125 132 1:CAS:528:DC%2BD3MXislaqs7w%3D 10.1016/S0306-4522(00)00565-0 11311793 (Pubitemid 32304352)
76. C Tornetta ZY Gao VM Lee BA Wof 1998 Regulation of amyloid precursor protein secretion by glutamate receptors in human Ntera 2 Neurons (NT2N)J Biol Chem 273 14015 14021 10.1074/jbc.273.22.14015 (Pubitemid 28268458)
77. M Tsacopoulos PJ Magistretti 1996 Metabolic coupling between glia and neurons J Neurosci 16 877 885 1:CAS:528:DyaK28XmsVenuw%3D%3D 8558256 (Pubitemid 26140843)
78. E Van Vliet S Morath C Eskes J Linge J Rappsilber P Honegger, et al. 2008 A novel in vitro metabolomics approach for neurotoxicity testing, proof of principle for methyl mercury chloride and caffeine Neurotoxicology 29 1 12 10.1016/j.neuro.2007.09.007 18023877
79. C Verderio M Matteoli 2001 ATP mediates calcium signaling between astrocytes and microglial cells: modulation by IFN-gamma J Immunol 166 6383 6391 1:CAS:528:DC%2BD3MXjsFCqtLg%3D 11342663 (Pubitemid 32440772)
80. SJ Wang 2007 Caffeine facilitation of glutamate release from rat cerebral cortex nerve terminals (synaptosomes) through activation protein kinase C pathway: an interaction with presynaptic adenosine A1 receptors Synapse 61 401 411 1:CAS:528:DC%2BD2sXktlKqu70%3D 10.1002/syn.20384 17372967 (Pubitemid 46559537)
81. LT Watts ML Rathinam S Schenker GI Henderson 2005 Astrocytes protect neurons from ethanol-induced oxidative stress and apoptotic death J Neurosci Res 80 655 666 1:CAS:528:DC%2BD2MXltFagtbo%3D 10.1002/jnr.20502 15880562 (Pubitemid 40896186)
82. ES Williams J Panko DJ Paustenbach 2009 The European Union's REACH regulation: a review of its history and requirements Critical Rev Toxicol 39 553 575 10.1080/10408440903036056
83. EK Woehrling EJ Hill MD Coleman 2007 Development of a neurotoxicity test-system, using human post-mitotic, astrocytic and neuronal cell lines in co-culture Toxicol In Vitro 21 1241 1246 1:CAS:528:DC%2BD2sXht1GrsL7J 10.1016/j.tiv.2007.04.011 17566694 (Pubitemid 350026239)
84. EK Woehrling EJ Hill MD Coleman 2010 Evaluation of the importance of astrocytes when screening for acute toxicity in neuronal cell systems Neurotox Res 17 103 113 1:CAS:528:DC%2BC3cXjt1Wqt7o%3D 10.1007/s12640-009-9084-3 19593679
85. U Wullner J Seyfried P Groscurth S Beinroth S Winter M Gleichmann, et al. 1999 Glutathione depletion and neuronal cell death: the role of reactive oxygen intermediates and mitochondrial function Brain Res 826 53 62 1:CAS:528:DyaK1MXisV2gs7w%3D 10.1016/S0006-8993(99)01228-7 10216196 (Pubitemid 29222959)
86. C Yi KQ Xie FY Song LH Yu XL Zhao GZ Li, et al. 2006 The changes of cytoskeletal proteins in plasma of acrylamide-induced rats Neurochem Res 31 751 757 1:CAS:528:DC%2BD28Xnt1aitLk%3D 10.1007/s11064-006-9079-x 16845587 (Pubitemid 44114809)
87. D Yildiz 2004 Nicotine, its metabolism and an overview of its biological effects Toxicon 43 619 632 1:CAS:528:DC%2BD2cXjtlyhs70%3D 10.1016/j.toxicon. 2004.01.017 15109883 (Pubitemid 38520766)
88. PH Yu DM Zuo 1997 Enhanced tolerance of neuroblastoma cells towards the neurotoxin 6-hydroxydopamine following specific cell-cell interaction with primary astrocytes Neuroscience 78 903 912 1:CAS:528:DyaK2sXjtVCgsLg%3D 10.1016/S0306-4522(96)00582-9 9153668 (Pubitemid 27179669)
89. SF Yu XL Zhao TL Zhang LH Yu SX Li N Cui, et al. 2005 Acrylamide-induced changes in the neurofilament protein of rat cerebrum fractions Neurochem Res 30 1079 1085 1:CAS:528:DC%2BD2MXht1Wqur%2FK 10.1007/s11064-005-7413-3 16292499 (Pubitemid 41642149)
90. SF Yu FY Son JX Yu XL Zhao LH Yu GZ Li, et al. 2006 Acrylamide alters cytoskeletal protein level in rat sciatic nerves Neurochem Res 31 1197 1204 1:CAS:528:DC%2BD28XhtFKmtr%2FN 10.1007/s11064-006-9176-x 17043767 (Pubitemid 44701766)
91. JL Zhou JH Liang JW Zheng CL Li 2004 Nerve growth factor protects R2 cells against neurotoxicity induced by methamphetamine Toxicol Lett 150 221 227 1:CAS:528:DC%2BD2cXjtF2ru7w%3D 10.1016/j.toxlet.2004.01.007 15093677 (Pubitemid 38490529)
92. LA Zimmer M Ennis RG Wiley MT Shipley 1998 Nerve gas-induced seizures: role of acetylcholine in the rapid induction of Fos and glial fibrillary acidic protein in piriform cortex J Neurosci 18 3897 3908 1:CAS:528:DyaK1cXjtVWqtbY%3D 9570817 (Pubitemid 28225694)
93. MG Zurich P Honegger B Shelter LG Costa F Monnet-Tschudi 2004 Involvement of glial cells in the neurotoxicity of parathion and chlorpyrifos Toxicol Appl Pharmacol 201 97 104 1:CAS:528:DC%2BD2cXpslCqtbc%3D 10.1016/j.taap.2004.05.003 15541749 (Pubitemid 39487157)