Detailed understanding of enhanced specific productivity in Chinese hamster ovary cells at low culture temperature

Journal of Bioscience and Bioengineering

Volumn 111, Issue 3, 2011, Pages 365-369

  Kou, Tian Ci ^a   Fan, Li ^a   Zhou, Yan ^a   Ye, Zhao Yang ^a   Liu, Xu Ping ^a   Zhao, Liang ^a   Tan, Wen Song ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Chinese hamster ovary (CHO) cells; Low temperature; MRNA stability; TNFR Fc; Transcriptional rate

Indexed keywords

CHINESE HAMSTER OVARY CELLS; LOW TEMPERATURE; MRNA STABILITY; TNFR-FC; TRANSCRIPTIONAL RATE;

GENES; NUCLEIC ACIDS; POLYMERASE CHAIN REACTION; PRODUCTIVITY; STABILITY; TRANSCRIPTION;

CELL CULTURE;

FC RECEPTOR; IMMUNOGLOBULIN G1; MESSENGER RNA; TUMOR NECROSIS FACTOR RECEPTOR;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL GROWTH; CELL KINETICS; CHO CELL; CONTROLLED STUDY; GENE DOSAGE; GENETIC TRANSCRIPTION; LOW TEMPERATURE; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; RNA STABILITY; TEMPERATURE SENSITIVITY;

ANIMALS; CELL CULTURE TECHNIQUES; CHO CELLS; COLD TEMPERATURE; CRICETINAE; CRICETULUS; GENE DOSAGE; IMMUNOGLOBULIN G; RECEPTORS, TUMOR NECROSIS FACTOR; RECOMBINANT FUSION PROTEINS; RNA STABILITY; RNA, MESSENGER; TRANSCRIPTION, GENETIC; TRANSFECTION;

CRICETULUS GRISEUS;

EID: 79951812696     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2010.11.016     Document Type: Article

References (27)

1. Dinnis D.M., James D.C. Engineering mammalian cell factories for improved recombinant monoclonal antibody production: lessons from nature. Biotechnol. Bioeng. 2005, 91:180-189.
2. Omasa T., Takami T., Ohya T., Kiyama E., Hayashi T., Nishii H., Miki H., Kobayashi K., Honda K., Ohtake H. Overexpression of GADD34 enhances production of recombinant human antithrombin in Chinese hamster ovary cells. J. Biosci. Bioeng. 2008, 106:568-573.
3. Liu C.H., Wu K.W. Synergistic effects of basic fibroblast growth factor and insulin on Chinese hamster ovary cells under serum-free conditions. J. Biosci. Bioeng. 2009, 107:312-317.
4. Xie L., Wang D.I.C. Fed-batch cultivation of animal cells using different medium design concepts and feeding strategies. Biotechnol. Bioeng. 2006, 95:270-284.
5. Spens E., Häggström L. Defined protein and animal component-free NS0 fed-batch culture. Biotechnol. Bioeng. 2007, 98:1183-1194.
6. Fan L., Zhao L., Ye Z.Y., Sun Y.T., Kou T.C., Zhou Y., Tan W.S. Effect of culture temperature on TNFR-Fc productivity in recombinant glutamine synthetase Chinese hamster ovary cells. Biotechnol. Lett. 2010, 32:1239-1244.
7. Kim Y.-G., Kim J.Y., Mohan C., Lee G.M. Effect of Bcl-xL overexpression on apoptosis and autophagy in recombinant Chinese hamster ovary cells under nutrient-deprived condition. Biotechnol. Bioeng. 2009, 103:757-766.
8. Liew J.C.J., Tan W.S., Alitheen N.B.M., Chan E.S., Tey B.T. Over-expression of the X-linked inhibitor of apoptosis protein (XIAP) delays serum deprivation-induced apoptosis in CHO-K1 cells. J. Biosci. Bioeng. 2010, 110:338-344.
9. Ahn W.S., Jeon J.-J., Jeong Y.-R., Lee S.J., Yoon S.K. Effect of culture temperature on erythropoietin production and glycosylation in a perfusion culture of recombinant CHO cells. Biotechnol. Bioeng. 2008, 101:1234-1244.
10. Yee J.C., de Leon Gatti M., Philp R.J., Yap M., Hu W.-S. Genomic and proteomic exploration of CHO and hybridoma cells under sodium butyrate treatment. Biotechnol. Bioeng. 2008, 99:1186-1204.
11. Zhao L., Fan L., Wang J.Q., Niu H.X., Tan W.S. Responses of GS-NSO myeloma cells to osmolality: cell growth, intracellular mass metabolism, energy metabolism, and antibody production. Biotechnol. Bioprocess. Eng. 2009, 14:625-632.
12. Fox S.R., Patel U.A., Yap M.G.S., Wang D.I.C. Maximizing interferon-γ production by chinese hamster ovary cells through temperature shift optimization: experimental and modeling. Biotechnol. Bioeng. 2004, 85:177-184.
13. Yoon S., Hong J., Choo S., Song J., Park H., Lee G. Adaptation of Chinese hamster ovary cells to low culture temperature: cell growth and recombinant protein production. J. Biotechnol. 2006, 122:463-472.
14. Trummer E., Fauland K., Seidinger S., Schriebl K., Lattenmayer C., Kunert R., Vorauer-Uhl K., Weik R., Borth N., Katinger H., Müller D. Process parameter shifting: Part II. Biphasic cultivation-a tool for enhancing the volumetric productivity of batch processes using Epo-Fc expressing CHO cells. Biotechnol. Bioeng. 2006, 94:1045-1052.
15. Chen Z. Temperature shift as a process optimization step for the production of pro-urokinase by a recombinant Chinese hamster ovary cell line in high-density perfusion culture. J. Biosci. Bioeng. 2004, 97:239-243.
16. Yoon S.K., Ahn Y.-H., Jeong M.H. Effect of culture temperature on follicle-stimulating hormone production by Chinese hamster ovary cells in a perfusion bioreactor. Appl. Microbiol. Biotechnol. 2007, 76:83-89.
17. Yoon S.K., Song J.Y., Lee G.M. Effect of low culture temperature on specific productivity, transcription level, and heterogeneity of erythropoietin in Chinese hamster ovary cells. Biotechnol. Bioeng. 2003, 82:289-298.
18. Baik J.Y., Lee M.S., An S.R., Yoon S.K., Joo E.J., Kim Y.H., Park H.W., Lee G.M. Initial transcriptome and proteome analyses of low culture temperature-induced expression in CHO cells producing erythropoietin. Biotechnol. Bioeng. 2006, 93:361-371.
19. Sun Z., Zhou R., Liang S.Y., McNeeley K.M., Sharfstein S.T. Hyperosmotic stress in murine hybridoma cells: effects on antibody transcription, translation, posttranslational processing, and the cell cycle. Biotechnol. Prog. 2004, 20:576-589.
20. Renard J.M., Spagnoli R., Mazier C., Salles M.F., Mandine E. Evidence that monoclonal antibody production kinetics is related to the integral of viable cells in batch systems. Biotechnol. Lett. 1988, 10:91-96.
21. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 1987, 162:156-159.
22. Wang W., Yi X., Zhang Y. Gene transcription acceleration: main cause of hepatitis B surface antigen production improvement by dimethyl sulfoxide in the culture of Chinese hamster ovary cells. Biotechnol. Bioeng. 2007, 97:526-535.
23. Goodman L.S., Gilman A. The pharmacological basis of therapeutics 1990, 1240-1241. Pergamon Press, Oxford.
24. Khoo S.H.G., Al-Rubeai M. Detailed understanding of enhanced specific antibody productivity in NSO myeloma cells. Biotechnol. Bioeng. 2009, 102:188-199.
25. Fann C.H., Guarna M.M., Kilburn D.G., Piret J.M. Relationship between recombinant activated protein C secretion rates and mRNA levels in baby hamster kidney cells. Biotechnol. Bioeng. 1999, 63:464-472.
26. Bibila T.A., Flickinger M.C. A structured model for monoclonal antibody synthesis in exponentially growing and stationary phase hybridoma cells. Biotechnol. Bioeng. 1991, 37:210-226.
27. Ross J. mRNA stability in mammalian cells. Microbiol. Rev. 1995, 59:423-450.