LPS ligand and culture additives improve production of monomeric MD-1 and 2 in Pichia pastoris by decreasing aggregation and intermolecular disulfide bonding

Protein Expression and Purification

Volumn 76, Issue 2, 2011, Pages 173-183

  Mengwasser, Kristen E ^a   Bryant, Clare E ^a   Gay, Nick J ^a   Gangloff, Monique ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Additive screening; Aggregation; Disulfide bonds; LPS binding; MD 1; MD 2; Pichia pastoris expression

Indexed keywords

DISULFIDE; HYBRID PROTEIN; LIGAND; LIPOPOLYSACCHARIDE; LY86 PROTEIN, HUMAN; LY96 PROTEIN, HUMAN; MEMBRANE ANTIGEN; PROTEIN MD 2;

ANIMAL; ARTICLE; BIOSYNTHESIS; CHEMISTRY; CULTURE MEDIUM; GEL CHROMATOGRAPHY; GENETICS; HORSE; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; PHENOTYPE; PICHIA; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN MULTIMERIZATION;

ANIMALS; ANTIGENS, SURFACE; CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; CULTURE MEDIA; DISULFIDES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HORSES; HUMANS; LIGANDS; LIPOPOLYSACCHARIDES; LYMPHOCYTE ANTIGEN 96; PHENOTYPE; PICHIA; PROTEIN MULTIMERIZATION; RECOMBINANT FUSION PROTEINS;

PICHIA PASTORIS;

EID: 79151485826     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.11.018     Document Type: Article

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