The structural basis of lipopolysaccharide recognition by the TLR4-MD-2 complex

Nature

Volumn 458, Issue 7242, 2009, Pages 1191-1195

  Park, Beom Seok ^a   Song, Dong Hyun ^a   Kim, Ho Min ^a   Choi, Byong Seok ^a   Lee, Hayyoung ^c   Lee, Jie Oh ^a,b  

^a Department of Chemistry ^*  (South Korea)

^b Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^c Chungnam National University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

LIPOPOLYSACCHARIDE; PHENYLALANINE; PROTEIN MD 2; TOLL LIKE RECEPTOR 4;

BACTERIUM; CRYSTAL STRUCTURE; HYDROPHOBICITY; IMMUNE RESPONSE; LIGAND; MOLECULAR ANALYSIS; SOLVENT;

ARTICLE; COMPLEX FORMATION; CRYSTAL STRUCTURE; HYDROGEN BOND; HYDROPHILICITY; HYDROPHOBICITY; MOLECULAR RECOGNITION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE;

BINDING SITES; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; HUMANS; HYDROPHOBICITY; LIPOPOLYSACCHARIDES; LYMPHOCYTE ANTIGEN 96; MODELS, MOLECULAR; PROTEIN BINDING; PROTEIN MULTIMERIZATION; STRUCTURE-ACTIVITY RELATIONSHIP; TOLL-LIKE RECEPTOR 4;

NEGIBACTERIA; TETRA;

EID: 67349182481     PISSN: 00280836     EISSN: 14764687     Source Type: Journal    
DOI: 10.1038/nature07830     Document Type: Article

References (30)

1. Beutler, B. & Rietschel, E. T. Innate immune sensing and its roots: the story of endotoxin. Nature Rev. Immunol. 3, 169-176 (2003).
2. Kim, H. M. et al. Crystal structure of the TLR4-MD-2 complex with bound endotoxin antagonist Eritoran. Cell 130, 906-917 (2007).
3. Ohto, U., Fukase, K., Miyake, K. & Satow, Y. Crystal structures of human MD-2 and its complex with antiendotoxic lipid IVa. Science 316, 1632-1634 (2007).
4. Jin, M. S. et al. Crystal structure of the TLR1-TLR2 heterodimer induced by binding of a tri-acylated lipopeptide. Cell 130, 1071-1082 (2007).
5. Liu, L. et al. Structural basis of toll-like receptor 3 signaling with double-stranded RNA. Science 320, 379-381 (2008).
6. Raetz, C. R. Biochemistry of endotoxins. Annu. Rev. Biochem. 59, 129-170 (1990).
7. Raetz, C. R. & Whitfield, C. Lipopolysaccharide endotoxins. Annu. Rev. Biochem. 71, 635-700 (2002).
8. Medzhitov, R., Preston-Hurlburt, P. & Janeway, C. A. Jr. A human homologue of the Drosophila Toll protein signals activation of adaptive immunity. Nature 388, 394-397 (1997).
9. Shimazu, R. et al. MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4. J. Exp. Med. 189, 1777-1782 (1999).
10. Matsushima, N. et al. Comparative sequence analysis of leucine-rich repeats (LRRs) within vertebrate toll-like receptors. BMC Genom. 8, 124 (2007).
11. Bell, J. K. et al. The molecular structure of the Toll-like receptor 3 ligand-binding domain. Proc. Natl Acad. Sci. USA 102, 10976-10980 (2005).
12. Choe, J., Kelker, M. S.&Wilson, I. A. Crystal structure of human toll-like receptor 3 (TLR3) ectodomain. Science 309, 581-585 (2005).
13. Miyake, K. Roles for accessory molecules in microbial recognition by Toll-like receptors. J. Endotoxin Res. 12, 195-204 (2006).
14. Erridge, C., Bennett-Guerrero, E. & Poxton, I. R. Structure and function of lipopolysaccharides. Microbes Infect. 4, 837-851 (2002).
15. Rietschel, E. T. et al. Bacterial endotoxin: molecular relationships of structure to activity and function. FASEB J. 8, 217-225 (1994).
16. Bella, J., Hindle, K. L., McEwan, P. A. & Lovell, S. C. The leucine-rich repeat structure. Cell. Mol. Life Sci. 65, 2307-2333 (2008).
17. Galanos, C. et al. Synthetic and natural Escherichia coli free lipidAexpress identical endotoxic activities. Eur. J. Biochem. 148, 1-5 (1985).
18. Kobayashi, M. et al. Regulatory roles for MD-2 and TLR4 in ligand-induced receptor clustering. J. Immunol. 176, 6211-6218 (2006).
19. Kawasaki, K., Nogawa, H. & Nishijima, M. Identification of mouse MD-2 residues important for forming the cell surface TLR4-MD-2 complex recognized by anti-TLR4-MD-2 antibodies, and for conferring LPS and taxol responsiveness on mouse TLR4 by alanine-scanning mutagenesis. J. Immunol. 170, 413-420 (2003).
20. Re, F. & Strominger, J. L. Separate functional domains of human MD-2 mediate Toll-like receptor 4-binding and lipopolysaccharide responsiveness. J. Immunol. 171, 5272-5276 (2003).
21. Visintin, A., Latz, E., Monks, B. G., Espevik, T. & Golenbock, D. T. Lysines 128 and 132 enable lipopolysaccharide binding to MD-2, leading to Toll-like receptor-4 aggregation and signal transduction. J. Biol. Chem. 278, 48313-48320 (2003).
22. Teghanemt, A. et al. Novel roles in human MD-2 of phenylalanines 121 and 126 and tyrosine 131 in activation of Toll-like receptor 4 by endotoxin. J. Biol. Chem. 283, 1257-1266 (2008).
23. Jin, M. S. & Lee, J. O. Structures of the Toll-like receptor family and its ligand complexes. Immunity 29, 182-191 (2008).
24. Rietschel, E. T. et al. The chemical structure of bacterial endotoxin in relation to bioactivity. Immunobiology 187, 169-190 (1993).
25. Teghanemt, A., Zhang, D., Levis, E. N., Weiss, J. P. & Gioannini, T. L. Molecular basis of reduced potency of underacylated endotoxins. J. Immunol. 175, 4669-4676 (2005).
26. Rossignol, D. P. & Lynn, M. TLR4 antagonists for endotoxemia and beyond. Curr. Opin. Investig. Drugs 6, 496-502 (2005).
27. Mata-Haro, V. et al. The vaccine adjuvant monophosphoryl lipid A as a TRIFbiased agonist of TLR4. Science 316, 1628-1632 (2007).
28. Ulmer, A. J. et al. Biological activity of synthetic phosphonooxyethyl analogs of lipid A and lipid A partial structures. Infect. Immun. 60, 3309-3314 (1992).
29. Walsh, C. et al. Elucidation of the MD-2/TLR4 interface required for signaling by lipid IVa. J. Immunol. 181, 1245-1254 (2008).
30. McCoy, A. J., Grosse-Kunstleve, R. W., Storoni, L. C. & Read, R. J. Likelihoodenhanced fast translation functions. Acta Crystallogr. D 61, 458-464 (2005).