Yeast-hybrid based high-throughput assay for identification of anthrax lethal factor inhibitors

Biochemical and Biophysical Research Communications

Volumn 404, Issue 1, 2011, Pages 517-522

  Kim, Joungmok ^a   Park, Hae Chul ^a   Gedi, Vinayakumar ^a   Park, Hye Yeon ^a   Roberts, Arthur G ^b   Atkins, William M ^b   Yoon, Moon Young ^a  

^a Hanyang University   (South Korea)

^b UNIVERSITY OF WASHINGTON   (United States)

Author keywords

Anthrax lethal factor; Cell based high throughput assay; Chemical library screening; Yeast

Indexed keywords

ANTHRAX LETHAL FACTOR INHIBITOR; ANTHRAX LETHAL TOXIN; ANTIINFECTIVE AGENT; BACTERIAL TOXIN; CBB 0348; CBT 0052; FBS 00831; FBS 08879; ILOMASTAT; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR GAL4; UNCLASSIFIED DRUG;

ANTHRAX; ARTICLE; BACILLUS ANTHRACIS; CELL PROTECTION; CELL TRANSFORMATION; CHIMERA; CONTROLLED STUDY; DNA BINDING; DRUG IDENTIFICATION; DRUG SCREENING; EUKARYOTIC CELL; GENE ACTIVITY; GENE EXPRESSION; GENE INSERTION; GENE SEQUENCE; HIGH THROUGHPUT SCREENING; HYBRID; IN VITRO STUDY; LETHAL GENE; MACROPHAGE; MOLECULAR MECHANICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; REPORTER GENE; YEAST; YEAST CELL;

ANIMALS; ANTHRAX; ANTIGENS, BACTERIAL; BACILLUS ANTHRACIS; BACTERIAL TOXINS; DNA-BINDING PROTEINS; GENES, REPORTER; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MACROPHAGES; MICE; PROTEASE INHIBITORS; PYRONES; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SMALL MOLECULE LIBRARIES; SULFHYDRYL COMPOUNDS; TRANSCRIPTION FACTORS; TWO-HYBRID SYSTEM TECHNIQUES;

EUKARYOTA;

EID: 78650861456     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2010.12.015     Document Type: Article

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