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Volumn 17, Issue 16, 2007, Pages 4575-4578

Inhibitors of anthrax lethal factor

Author keywords

Anthrax; Inhibitor; Lethal factor

Indexed keywords

ANTHRAX TOXIN; DOPAMINE DERIVATIVE; OLEOYL DOPAMINE; UNCLASSIFIED DRUG;

EID: 34447307647     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2007.05.089     Document Type: Article
Times cited : (23)

References (32)
  • 22
    • 34447331045 scopus 로고    scopus 로고
    • note
    • Lethal toxin challenge conditions: J774A.1 cells were allowed to grow to confluency in 96-well plates before 3 nM PA, LOPAC 1280 (Sigma) library members or compounds 1-6 (0.6% DMSO), and 0.61 nM LF (List Biological Laboratories) were added to the cells. Cells were allowed to incubate with the PA/LF/compound mixture for 4 h at 37 °C before MTT was added. Cells were allowed to incubate for an additional 1.5 h at 37 °C before cell viability was determined.
  • 24
    • 34447324419 scopus 로고    scopus 로고
    • note
    • 50): compounds 1-6 (0.6% DMSO) were incubated with 50 nM LF (List Biological Laboratories) in assay buffer (65 μL) for 1 h before addition to 4 μM MAPKKide (List Biological Laboratories) in assay buffer (10 μL). Fluorescent measurements were performed over 10 min (excitation and emission of 485 and 590, respectively). Assay buffer consisted of 20 mM Hepes, pH 7.4.
  • 26
    • 34447315310 scopus 로고    scopus 로고
    • note
    • i): compounds 1, 4 and 6 (1.6% DMSO) were incubated with 12.5 nM LF (List Biological Laboratories) in assay buffer (65 μL) for 1 h before addition to 6-12 μM MAPKKide (List Biological Laboratories) in assay buffer (10 μL). Fluorescent measurements were performed over 10 min (excitation and emission of 485 and 590, respectively). Assay buffer consisted of 20 mM Hepes, pH 7.4.
  • 29
    • 34447333150 scopus 로고    scopus 로고
    • note
    • +).
  • 30
    • 34447339418 scopus 로고    scopus 로고
    • note
    • +).
  • 31
    • 34447333421 scopus 로고    scopus 로고
    • note
    • Cytotoxicity study: J774A.1 cells were allowed to grow to confluency in 96-well plates before 0-100 μM compound in media (0.6% DMSO) was added to the cells. Cells were allowed to incubate with the compound mixture for 4 h at 37 °C before MTT was added. Cells were allowed to incubate for an additional 1.5 h at 37 °C before cell viability was determined.
  • 32
    • 34447324696 scopus 로고    scopus 로고
    • note
    • +).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.