Studies of ion channels using expressed protein ligation

Current Opinion in Chemical Biology

Volumn 14, Issue 6, 2010, Pages 797-802

  Focke, Paul J ^a   Valiyaveetil, Francis I ^a  

^a OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ION CHANNEL; MEMBRANE PROTEIN; OUTER MEMBRANE PROTEIN F; POTASSIUM CHANNEL; PROTEIN; SODIUM CHANNEL; SYNTHETIC PEPTIDE;

AMINO ACID SUBSTITUTION; ARTICLE; BIOTECHNOLOGY; NANOTECHNOLOGY; PROTEIN MODIFICATION; PROTEIN SYNTHESIS;

AMIDES; ESTERS; HUMANS; ION CHANNELS; PROTEIN ENGINEERING;

EID: 78649827915     PISSN: 13675931     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cbpa.2010.09.014     Document Type: Article

References (49)

1. White S.H. Biophysical dissection of membrane proteins. Nature 2009, 459:344-346.
2. Cherezov V., Rosenbaum D.M., Hanson M.A., Rasmussen S.G., Thian F.S., Kobilka T.S., Choi H.J., Kuhn P., Weis W.I., Kobilka B.K., et al. High-resolution crystal structure of an engineered human beta2-adrenergic G protein-coupled receptor. Science 2007, 318:1258-1265.
3. Sobolevsky A.I., Rosconi M.P., Gouaux E. X-ray structure, symmetry and mechanism of an AMPA-subtype glutamate receptor. Nature 2009, 462:745-756.
4. Tao X., Avalos J.L., Chen J., MacKinnon R. Crystal structure of the eukaryotic strong inward-rectifier K+ channel Kir2.2 at 3.1 A resolution. Science 2009, 326:1668-1674.
5. Wang L., Schultz P.G. Expanding the genetic code. Angew Chem Int Ed Engl 2004, 44:34-66.
6. Kent S.B. Total chemical synthesis of proteins. Chem Soc Rev 2009, 38:338-351.
7. Muralidharan V., Muir T.W. Protein ligation: an enabling technology for the biophysical analysis of proteins. Nat Methods 2006, 3:429-438.
8. Liu C.C., Schultz P.G. Adding new chemistries to the genetic code. Annu Rev Biochem 2010, 79:413-444.
9. Young T.S., Schultz P.G. Beyond the canonical 20 amino acids: expanding the genetic lexicon. J Biol Chem 2010, 285:11039-11044.
10. Kent S. Chemical synthesis of peptides and proteins. Annu Rev Biochem 1988, 57:957-989.
11. Dawson P.E., Muir T.W., Clarklewis I., Kent S.B.H. Synthesis of proteins by native chemical ligation. Science 1994, 266:776-779.
12. Dawson P.E., Kent S.B.H. Synthesis of native proteins by chemical ligation. Annu Rev Biochem 2000, 69:923-960.
13. Muir T.W., Sondhi D., Cole P.A. Expressed protein ligation: a general method for protein engineering. Proc Natl Acad Sci USA 1998, 95:6705-6710.
14. Muir T. Semisynthesis of proteins by expressed protein ligation. Annu Rev Biochem 2003, 72:249-289.
15. Berrade L., Camarero J.A. Expressed protein ligation: a resourceful tool to study protein structure and function. Cell Mol Life Sci 2009, 66:3909-3922.
16. Shi N., Ye S., Alam A., Chen L.P., Jiang Y. Atomic structure of a Na+- and K+-conducting channel. Nature 2006, 440:570-574.
17. Alam A., Shi N., Jiang Y. Structural insight into Ca2+ specificity in tetrameric cation channels. Proc Natl Acad Sci USA 2007, 104:15334-15339.
18. Shi N., Ye S., Alam A., Chen L., Jiang Y. Atomic structure of a Na+- and K+-conducting channel. Nature 2006, 440:570-574.
19. Alam A., Jiang Y. Structural analysis of ion selectivity in the NaK channel. Nat Struct Mol Biol 2009, 16:35-41.
20. Alam A., Jiang Y. High-resolution structure of the open NaK channel. Nat Struct Mol Biol 2009, 16:30-34.
21. Zhou Y., Morais-Cabral J., Kaufman A., MacKinnon R. Chemistry of ion coordination and hydration revealed by a K+ channel-Fab complex at 2.0 A resolution. Nature 2001, 414:43-48.
22. Jiang Y., Lee A., Chen J., Ruta V., Cadene M., Chait B., MacKinnon R. X-ray structure of a voltage-dependent K+ channel. Nature 2003, 423:33-41.
23. Long S.B., Tao X., Campbell E.B., MacKinnon R. Atomic structure of a voltage-dependent K+ channel in a lipid membrane-like environment. Nature 2007, 450:376-382.
24. Linn K.M., Derebe M.G., Jiang Y., Valiyaveetil F.I. Semisynthesis of NaK, a Na(+) and K(+) conducting ion channel. Biochemistry 2010, 49:4450-4456.
25. Johnson E., Kent S. Towards the total chemical synthesis of integral membrane proteins: a general method for the synthesis of hydrophobic peptide-thioester building blocks. Tetrahedron Lett 2007, 48:1795-1799.
26. Sato T., Saito Y., Aimoto S. Synthesis of the C-terminal region of opioid receptor like 1 in an SDS micelle by the native chemical ligation: effect of thiol additive and SDS concentration on ligation efficiency. J Pept Sci 2005, 11:410-416.
27. Valiyaveetil F., MacKinnon R., Muir T. Semisynthesis and folding of the potassium channel KcsA. J Am Chem Soc 2002, 124:9113-9120.
28. Frangioni J.V., Neel B.G. Solubilization and purification of enzymatically active glutathione-S-transferase (pGEX) fusion proteins. Anal Biochem 1993, 210:179-187.
29. Southworth M.W., Amaya K., Evans T.C., Xu M.Q., Perler F.B. Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein. Biotechniques 1999, 1999:110-114.
30. Schirmer T. General and specific porins from bacterial outer membranes. J Struct Biol 1998, 121:101-109.
31. Cowan S.W., Schirmer T., Rummel G., Steiert M., Ghosh R., Pauptit R.A., Jansonius J.N., Rosenbusch J.P. Crystal structures explain functional properties of two E. coli porins. Nature 1992, 358:727-733.
32. Reitz S., Cebi M., Reiss P., Studnik G., Linne U., Koert U., Essen L.O. On the function and structure of synthetically modified porins. Angew Chem Int Ed Engl 2009, 48:4853-4857.
33. Komarov A.G., Linn K.M., Devereaux J.J., Valiyaveetil F.I. Modular strategy for the semisynthesis of a K+ channel: investigating interactions of the pore helix. ACS Chem Biol 2009, 4:1029-1038.
34. Valiyaveetil F.I., Sekedat M., Muir T.W., MacKinnon R. Semisynthesis of a functional K+ channel. Angew Chem, Int Ed 2004, 43:2504-2507.
35. Malakhov M.P., Mattern M.R., Malakhova O.A., Drinker M., Weeks S.D., Butt T.R. SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins. J Struct Funct Genomics 2004, 5:75-86.
36. Zuo X., Li S., Hall J., Mattern M., Tran H., Shoo J., Tan R., Weiss S., Butt T. Enhanced expression and purification of membrane proteins by SUMO fusion in Escherichia coli. J Struct Funct Genomics 2005, 6:103-111.
37. Bang D., Kent S.B.H. A one-pot total synthesis of crambin. Angew Chem, Int Ed 2004, 43:2534-2538.
38. Valiyaveetil F., Zhou Y., MacKinnon R. Lipids in the structure, folding, and function of the KcsA K+ channel. Biochemistry 2002, 41:10771-10777.
39. Zhou Y.F., MacKinnon R. The occupancy of ions in the K+ selectivity filter: charge balance and coupling of ion binding to a protein conformational change underlie high conduction rates. J Mol Biol 2003, 333:965-975.
40. Valiyaveetil F.I., Leonetti M., Muir T.W., MacKinnon R. Ion selectivity in a semisynthetic K+ channel locked in the conductive conformation. Science 2006, 314:1004-1007.
41. Doyle D.A., Cabral J.M., Pfuetzner R.A., Kuo A.L., Gulbis J.M., Cohen S.L., Chait B.T., MacKinnon R. The structure of the potassium channel: molecular basis of K+ conduction and selectivity. Science 1998, 280:69-77.
42. Valiyaveetil F., Sekedat M., MacKinnon R., Muir T. Structural and functional consequences of an amide-to-ester substitution in the selectivity filter of a potassium channel. J Am Chem Soc 2006, 128:11591-11599.
43. Lide D.R. CRC Handbook of Chemistry and Physics 1990, CRC Press, Inc., Boca Raton, FL. edn 71.
44. Vorherr T., Wrzosek A., Chiesi M., Carafoli E. Total synthesis and functional properties of the membrane-intrinsic protein phospholamban. Protein Sci 1993, 2:339-347.
45. Kochendoerfer G.G., Salom D., Lear J.D., Wilk-Orescan R., Kent S.B., DeGrado W.F. Total chemical synthesis of the integral membrane protein influenza A virus M2: role of its C-terminal domain in tetramer assembly. Biochemistry 1999, 38:11905-11913.
46. Kochendoerfer G.G., Jones D.H., Lee S., Oblatt-Montal M., Opella S.J., Montal M. Functional characterization and NMR spectroscopy on full-length Vpu from HIV-1 prepared by total chemical synthesis. J Am Chem Soc 2004, 126:2439-2446.
47. Clayton D., Shapovalov G., Maurer J., Dougherty D., Lester H., Kochendoerfer G. Total chemical synthesis and electrophysiological characterization of mechanosensitive channels from Escherichia coli and Mycobacterium tuberculosis. Proc Natl Acad Sci USA 2004, 101:4764-4769.
48. Bianchi E., Ingenito R., Simon R.J., Pessi A. Engineering and chemical synthesis of a transmembrane protein: the HCV protease cofactor protein NS4A. J Am Chem Soc 1999, 121:7698-7699.
49. Valiyaveetil F.I., Sekedat M., Mackinnon R., Muir T.W. Glycine as a d-amino acid surrogate in the K(+)-selectivity filter. Proc Natl Acad Sci USA 2004, 101:17045-17049.