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Volumn 121, Issue 33, 1999, Pages 7698-7699

Engineering and chemical synthesis of a transmembrane protein: The HCV protease cofactor protein NS4A [2]

Author keywords

[No Author keywords available]

Indexed keywords

MEMBRANE PROTEIN; SERINE PROTEINASE; SYNTHETIC PEPTIDE; VIRUS ENZYME; VIRUS PROTEIN;

EID: 0033603896     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja9901617     Document Type: Letter
Times cited : (36)

References (30)
  • 12
    • 0344823442 scopus 로고    scopus 로고
    • personal communication
    • R. De Francesco, personal communication.
    • De Francesco, R.1
  • 15
    • 0025042614 scopus 로고
    • The software used in our calculations is contained in the package "Peptide Companion", written by M. Lebl, G. Lebl, and V. Krchnak, commercially available from Peptides Int.: Louisville, KY
    • Milton, R. C. de L.; Milton, S. C. F.; Adams, P. A. J. Am. Chem. Soc. 1990, 112, 6039-6045. The software used in our calculations is contained in the package "Peptide Companion", written by M. Lebl, G. Lebl, and V. Krchnak, commercially available from Peptides Int.: Louisville, KY.
    • (1990) J. Am. Chem. Soc. , vol.112 , pp. 6039-6045
    • Milton, R.C.De.L.1    Milton, S.C.F.2    Adams, P.A.3
  • 18
    • 0344391705 scopus 로고    scopus 로고
    • note
    • Assessed by dynamic light scattering on a Dyna Pro-801 instrument, using a 1 mM solution of NS4A in 10% glycerol, 2% β-octyl-glucoside.
  • 26
    • 0344391704 scopus 로고    scopus 로고
    • note
    • Buffer composition: 25 mM HEPES, pH 7.0, 2% (v/v) β-octyl-glucoside, plus 1% (v/v) thiophenol; buffer combinations which proved unsuccessful due to target protein precipitation were 2-6 M guanidine hydrochloride with or without addition of organic cosolvents such as MeCN, dioxane, and DMF.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.