Knockout of exogenous EGFP gene in porcine somatic cells using zinc-finger nucleases

Biochemical and Biophysical Research Communications

Volumn 402, Issue 1, 2010, Pages 14-18

  Watanabe, Masahito ^a,b   Umeyama, Kazuhiro ^b   Matsunari, Hitomi ^b   Takayanagi, Shuko ^a,b   Haruyama, Erika ^b   Nakano, Kazuaki ^b   Fujiwara, Tsukasa ^b   Ikezawa, Yuka ^b   Nakauchi, Hiromitsu ^a,c   Nagashima, Hiroshi ^a,b  

^a JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^b MEIJI UNIVERSITY   (Japan)

^c THE UNIVERSITY OF TOKYO   (Japan)

Author keywords

Gene disruption; Knockout; Pig; Porcine somatic cells; Zinc finger nuclease(s)

Indexed keywords

DNA; ENHANCED GREEN FLUORESCENT PROTEIN; MESSENGER RNA; NUCLEASE; UNCLASSIFIED DRUG; ZINC FINGER NUCLEASE; ZINC FINGER PROTEIN;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; ELECTROPORATION; ENZYME ASSAY; FETUS; FLOW CYTOMETRY; FLUORESCENCE; GENE EXPRESSION; INDEL MUTATION; KNOCKOUT GENE; NONHUMAN; NUCLEIC ACID BASE SUBSTITUTION; PRIORITY JOURNAL; SOMATIC CELL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; BASE SEQUENCE; CELLS, CULTURED; DEOXYRIBONUCLEASES; DNA CLEAVAGE; DNA MUTATIONAL ANALYSIS; ELECTROPORATION; GENE KNOCKOUT TECHNIQUES; GREEN FLUORESCENT PROTEINS; MOLECULAR SEQUENCE DATA; SUS SCROFA; ZINC FINGERS;

ANIMALIA; SUIDAE; SUS;

EID: 78049251841     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2010.09.092     Document Type: Article

References (33)

1. Austin C.P., Battey J.F., Bradley A., et al. The knockout mouse project. Nat. Genet. 2004, 36:921-924.
2. Capecchi M.R. Altering the genome by homologous recombination. Science 1989, 244:1288-1292.
3. Guan C., Ye C., Yang X., et al. A review of current large-scale mouse knockout efforts. Genesis 2010, 48:73-85.
4. Itzhaki J.E., Porter A.C. Targeted disruption of a human interferon-inducible gene detected by secretion of human growth hormone. Nucleic Acids Res. 1991, 19:3835-3842.
5. Porter A.C., Itzhaki J.E. Gene targeting in human somatic cells. Complete inactivation of an interferon-inducible gene. Eur. J. Biochem. 1993, 218:273-281.
6. Brown J.P., Wei W., Sedivy J.M. Bypass of senescence after disruption of p21CIP1/WAF1 gene in normal diploid human fibroblasts. Science 1997, 277:831-834.
7. Dai Y., Vaught T.D., Boone J., et al. Targeted disruption of the alpha1,3-galactosyltransferase gene in cloned pigs. Nat. Biotechnol. 2002, 20:251-255.
8. Lai L., Kolber-Simonds D., Park K.W., et al. Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning. Science 2002, 295:1089-1092.
9. Rogers C.S., Stoltz D.A., Meyerholz D.K., et al. Disruption of the CFTR gene produces a model of cystic fibrosis in newborn pigs. Science 2008, 321:1837-1841.
10. Kues W.A., Niemann H. The contribution of farm animals to human health. Trends Biotechnol. 2004, 22:286-294.
11. Lunney J.K. Advances in swine biomedical model genomics. Int. J. Biol. Sci. 2007, 3:179-184.
12. Takahagi Y., Fujimura T., Miyagawa S., et al. Production of α1,3-galactosyltransferase gene knockout pigs expressing both human decay-accelerating factor and N-acetylglucosaminyltransferase III. Mol. Reprod. Dev. 2005, 71:331-338.
13. Umeyama K., Watanabe M., Saito H., et al. Dominant-negative mutant hepatocyte nuclear factor 1 alpha induces diabetes in transgenic-cloned pigs. Transgenic Res. 2009, 18:697-706.
14. Kragh P.M., Nielsen A.L., Li J., et al. Hemizygous minipigs produced by random gene insertion and handmade cloning express the Alzheimer's disease-causing dominant mutation APPsw. Transgenic Res. 2009, 18:545-558.
15. Matsunari H., Nagashima H. Application of genetically modified and cloned pigs in translational research. J. Reprod. Dev. 2009, 55:225-230.
16. Aigner B., Renner S., Kessler B., et al. Transgenic pigs as models for translational biomedical research. J. Mol. Med. 2010, 88:653-664.
17. Geurts A.M., Cost G.J., Freyvert Y., et al. Knockout rats via embryo microinjection of zinc-finger nucleases. Science 2009, 325:433.
18. Mashimo T., Takizawa A., Voigt B., et al. Generation of knockout rats with X-linked severe combined immunodeficiency (X-SCID) using zinc-finger nucleases. PLoS One 2010, 5:e8870.
19. Kurome M., Hisatomi H., Matsumoto S., et al. Production efficiency and telomere length of the cloned pigs following serial somatic cell nuclear transfer. J. Reprod. Dev. 2008, 54:254-258.
20. Matsunari H., Onodera M., Tada N., et al. Transgenic-cloned pigs systemically expressing red fluorescent protein, Kusabira-Orange. Cloning Stem Cells 2008, 10:313-323.
21. Okabe M., Ikawa M., Kominami K., et al. 'Green mice' as a source of ubiquitous green cells. FEBS Lett. 1997, 407:313-319.
22. Qiu P., Shandilya H., D'Alessio J.M., et al. Mutation detection using Surveyor nuclease. Biotechniques 2004, 36:702-707.
23. Urnov F.D., Miller J.C., Lee Y.L., et al. Highly efficient endogenous human gene correction using designed zinc-finger nucleases. Nature 2005, 435:646-651.
24. Beumer K.J., Trautman J.K., Bozas A., et al. Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases. Proc. Natl. Acad. Sci. USA 2008, 105:19821-19826.
25. Rémy S., Tesson L., Ménoret S., et al. Zinc-finger nucleases: a powerful tool for genetic engineering of animals. Transgenic Res. 2010, 19:363-371.
26. Miller J.C., Holmes M.C., Wang J., et al. An improved zinc-finger nuclease architecture for highly specific genome editing. Nat. Biotechnol. 2007, 25:778-785.
27. Doyon Y., McCammon J.M., Miller J.C., et al. Heritable targeted gene disruption in zebrafish using designed zinc finger nucleases. Nat. Biotechnol. 2008, 26:702-708.
28. Santiago Y., Chan E., Liu P.Q., et al. Targeted gene knockout in mammalian cells by using engineered zinc-finger nucleases. Proc. Natl. Acad. Sci. USA 2008, 105:5809-5814.
29. Kim H.J., Lee H.J., Kim H., et al. Targeted genome editing in human cells with zinc finger nucleases constructed via modular assembly. Genome Res. 2009, 19:1279-1288.
30. Zhang G., Gurtu V., Kain S.R. An enhanced green fluorescent protein allows sensitive detection of gene transfer in mammalian cells. Biochem. Biophys. Res. Commun. 1996, 227:707-711.
31. Yang F., Moss L.G., Phillips G.N. The molecular structure of green fluorescent protein. Nat. Biotechnol. 1996, 14:1246-1251.
32. Frischmeyer P.A., Dietz H.C. Nonsense-mediated mRNA decay in health and disease. Hum. Mol. Genet. 1999, 8:1893-1900.
33. Jeggo P.A. DNA breakage and repair. Adv. Genet. 1998, 38:185-218.