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Volumn 277, Issue 5327, 1997, Pages 831-834

Bypass of senescenoe after disruption of p21(CIP1)/(WAF1) gene in normal diploid human fibroblasts

Author keywords

[No Author keywords available]

Indexed keywords

CYCLIN DEPENDENT KINASE INHIBITOR; PROTEIN P21; ASPARTATE CARBAMOYLTRANSFERASE; CAD TRIFUNCTIONAL ENZYME; CARBAMOYL PHOSPHATE SYNTHASE (GLUTAMINE HYDROLYSING); CDKN1A PROTEIN, HUMAN; CYCLIN DEPENDENT KINASE INHIBITOR 1A; CYCLINE; DIHYDROOROTASE; MULTIENZYME COMPLEX;

EID: 0030609861     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.277.5327.831     Document Type: Article
Times cited : (697)

References (41)
  • 1
    • 0344622606 scopus 로고    scopus 로고
    • L. Hayflick and P. S. Moorhead, Exp. Cell Res. 25, 585 (1961); L. Hayflick, ibid. 37, 614 (1965); J. Campisi, G. Dimri, E. Hara, in Handbook of the Biology of Aging (Academic Press, New York, 1996), pp. 121-149; P. J. Vojta and J. C. Barrett, Biochim. Biophys. Acta 1242, 29 (1996).
    • (1961) Exp. Cell Res. , vol.25 , pp. 585
    • Hayflick, L.1    Moorhead, P.S.2
  • 2
    • 50549218525 scopus 로고
    • L. Hayflick and P. S. Moorhead, Exp. Cell Res. 25, 585 (1961); L. Hayflick, ibid. 37, 614 (1965); J. Campisi, G. Dimri, E. Hara, in Handbook of the Biology of Aging (Academic Press, New York, 1996), pp. 121-149; P. J. Vojta and J. C. Barrett, Biochim. Biophys. Acta 1242, 29 (1996).
    • (1965) Exp. Cell Res. , vol.37 , pp. 614
    • Hayflick, L.1
  • 3
    • 0344622606 scopus 로고    scopus 로고
    • Academic Press, New York
    • L. Hayflick and P. S. Moorhead, Exp. Cell Res. 25, 585 (1961); L. Hayflick, ibid. 37, 614 (1965); J. Campisi, G. Dimri, E. Hara, in Handbook of the Biology of Aging (Academic Press, New York, 1996), pp. 121-149; P. J. Vojta and J. C. Barrett, Biochim. Biophys. Acta 1242, 29 (1996).
    • (1996) Handbook of the Biology of Aging , pp. 121-149
    • Campisi, J.1    Dimri, G.2    Hara, E.3
  • 4
    • 0344622606 scopus 로고    scopus 로고
    • L. Hayflick and P. S. Moorhead, Exp. Cell Res. 25, 585 (1961); L. Hayflick, ibid. 37, 614 (1965); J. Campisi, G. Dimri, E. Hara, in Handbook of the Biology of Aging (Academic Press, New York, 1996), pp. 121-149; P. J. Vojta and J. C. Barrett, Biochim. Biophys. Acta 1242, 29 (1996).
    • (1996) Biochim. Biophys. Acta , vol.1242 , pp. 29
    • Vojta, P.J.1    Barrett, J.C.2
  • 6
    • 0029924534 scopus 로고    scopus 로고
    • C. B. Harley, A. B. Futcher, C. W. Greider, Nature 345, 458 (1990); H. Varizi and S. Benchimol, Exp. Gerontol. 31, 295 (1996).
    • (1996) Exp. Gerontol. , vol.31 , pp. 295
    • Varizi, H.1    Benchimol, S.2
  • 7
    • 0028924516 scopus 로고
    • K. S. Kulju and J. M. Lehman, Exp. Cell Res. 217, 336 (1995); J. A. Bond, M. Haughton, J. Blaydes, D. Wynford-Thomas, F. S. Wyllie, Oncogene 13, 2097 (1996).
    • (1995) Exp. Cell Res. , vol.217 , pp. 336
    • Kulju, K.S.1    Lehman, J.M.2
  • 11
    • 0028946009 scopus 로고
    • A. Noda, Y. Ning, S. F. Venable, O. M. Pereira-Smith, J. R. Smith, Exp. Cell Res. 211, 90 (1994); H. Tahara, E. Sato, A. Noda, T. Ide, Oncogene 10, 835 (1995).
    • (1995) Oncogene , vol.10 , pp. 835
    • Tahara, H.1    Sato, E.2    Noda, A.3    Ide, T.4
  • 12
    • 0028981380 scopus 로고
    • E. M. Rogan et al., Mol. Cell. Biol. 15, 4745 (1995); D. A. Alcorta et al., Proc. Natl. Acad. Sci. U.S.A. 93, 13742 (1996); E. Hara et al., Mol. Cell. Biol. 16, 859 (1996); M. Serrano et al., Cell 85, 27 (1996); H. Wong and K. Riabowol, Exp. Gerontol. 31, 311 (1996).
    • (1995) Mol. Cell. Biol. , vol.15 , pp. 4745
    • Rogan, E.M.1
  • 13
    • 0030477686 scopus 로고    scopus 로고
    • E. M. Rogan et al., Mol. Cell. Biol. 15, 4745 (1995); D. A. Alcorta et al., Proc. Natl. Acad. Sci. U.S.A. 93, 13742 (1996); E. Hara et al., Mol. Cell. Biol. 16, 859 (1996); M. Serrano et al., Cell 85, 27 (1996); H. Wong and K. Riabowol, Exp. Gerontol. 31, 311 (1996).
    • (1996) Proc. Natl. Acad. Sci. U.S.A. , vol.93 , pp. 13742
    • Alcorta, D.A.1
  • 14
    • 0029670840 scopus 로고    scopus 로고
    • E. M. Rogan et al., Mol. Cell. Biol. 15, 4745 (1995); D. A. Alcorta et al., Proc. Natl. Acad. Sci. U.S.A. 93, 13742 (1996); E. Hara et al., Mol. Cell. Biol. 16, 859 (1996); M. Serrano et al., Cell 85, 27 (1996); H. Wong and K. Riabowol, Exp. Gerontol. 31, 311 (1996).
    • (1996) Mol. Cell. Biol. , vol.16 , pp. 859
    • Hara, E.1
  • 15
    • 0029993450 scopus 로고    scopus 로고
    • E. M. Rogan et al., Mol. Cell. Biol. 15, 4745 (1995); D. A. Alcorta et al., Proc. Natl. Acad. Sci. U.S.A. 93, 13742 (1996); E. Hara et al., Mol. Cell. Biol. 16, 859 (1996); M. Serrano et al., Cell 85, 27 (1996); H. Wong and K. Riabowol, Exp. Gerontol. 31, 311 (1996).
    • (1996) Cell , vol.85 , pp. 27
    • Serrano, M.1
  • 16
    • 0029944622 scopus 로고    scopus 로고
    • E. M. Rogan et al., Mol. Cell. Biol. 15, 4745 (1995); D. A. Alcorta et al., Proc. Natl. Acad. Sci. U.S.A. 93, 13742 (1996); E. Hara et al., Mol. Cell. Biol. 16, 859 (1996); M. Serrano et al., Cell 85, 27 (1996); H. Wong and K. Riabowol, Exp. Gerontol. 31, 311 (1996).
    • (1996) Exp. Gerontol. , vol.31 , pp. 311
    • Wong, H.1    Riabowol, K.2
  • 18
    • 0027382325 scopus 로고
    • O. Sugawara, M. Oshimura, M. Koi, L. A. Annab, J. C. Barrett, Science 247, 707 (1990); T. Ogata et al., Mol. Cell. Biol. 13, 6036 (1993).
    • (1993) Mol. Cell. Biol. , vol.13 , pp. 6036
    • Ogata, T.1
  • 22
    • 1842326956 scopus 로고    scopus 로고
    • note
    • 2. Batches of FBS were tested for maximum single-cell cloning efficiency. FBS was added to medium immediately before use. Cultures were always passaged 1:4 at <80% confluence by brief treatment with 0.25% trypsin (without EDTA) and minimal pipetting. These conditions produce 30 to 50% single-cell cloning efficiency.
  • 23
    • 0003476394 scopus 로고
    • P. F. Kruse and M. K. Patterson, Eds. Academic Press, New York
    • Lung fibroblasts were explanted from a first-trimester female embryo [A. E. Greene, in Tissue Culture Methods and Applications, P. F. Kruse and M. K. Patterson, Eds. (Academic Press, New York, 1973), pp. 69-72]. The primary culture was designated passage 0.
    • (1973) Tissue Culture Methods and Applications , pp. 69-72
    • Greene, A.E.1
  • 24
    • 0028849274 scopus 로고
    • The neo-targeting vector had 5́ and 3́ flanks of 0.8 and 7.0 kbp, respectively. The hyg-targeting vector [T. Waldman, K. W. Kinzler, B. Vogelstein, Cancer Res. 55, 5187 (1995)] had 5́ and 3́ flanks of 4.6 and 4.0 kbp, respectively. Neither vector contained the p21 promoter or negatively selectable markers. Electroporation conditions; 0.8 ml of F10 medium (without serum), 0.4-cm cuvette, 20°C, 260 V, 975 μF, Bio-Rad Gene Pulser II apparatus. These conditions produce 6% transient electroporation efficiency.
    • (1995) Cancer Res. , vol.55 , pp. 5187
    • Waldman, T.1    Kinzler, K.W.2    Vogelstein, B.3
  • 25
    • 1842411441 scopus 로고    scopus 로고
    • note
    • HE and HO indicate heterozygous and homozygous derivatives, respectively. Nontargeted siblings are identified by clone number only. LF1 and HO7.2-1 cells tested negative for mycoplasma, human immunodeficiency virus, and HPV, and their karyotypes were normal.
  • 27
    • 0026771287 scopus 로고
    • Each round of targeting was performed with a different nonisogenic vector [H. te Riele, E. R. Maandag, A. Berns, Proc. Natl. Acad. Sci. U.S.A. 89, 5128 (1992)]. We have also targeted the p53 gene with efficiencies of 5. 6, and 33% in three separate experiments with two different HDF cell strains and isogenic as well as nonisogenic vectors (15). These results indicate that isogenic vectors are not necessary for gene targeting in human cells and that our targeting strategy is neither locus-nor cell strain-specific.
    • (1992) Proc. Natl. Acad. Sci. U.S.A. , vol.89 , pp. 5128
    • Te Riele, H.1    Maandag, E.R.2    Berns, A.3
  • 28
    • 0029047362 scopus 로고
    • Cultures were considered senescent when 1 PD took longer than 2 weeks. Senescent cultures were >95% β-galactosidase-positive [G. P. Dimri et al., Proc. Natl. Acad. Sci. U.S.A. 92, 9363 (1995)] and could be maintained for over 2 months without a significant decrease in cell number (15).
    • (1995) Proc. Natl. Acad. Sci. U.S.A. , vol.92 , pp. 9363
    • Dimri, G.P.1
  • 29
    • 1842337007 scopus 로고    scopus 로고
    • note
    • Passage number after gene targeting was arbitrarily set to 0 for each cell strain when the culture was first expanded into a 10-cm culture dish.
  • 30
    • 1842416720 scopus 로고    scopus 로고
    • note
    • Low-level mitotic activity coexisted with cell death; cells of variable size, irregular shape, and a significant number of large multinucleated cells were observed; cultures gradually declined over 6 to 8 weeks (15).
  • 33
    • 0027163464 scopus 로고
    • J. W. Shay, W. E. Wright, H. Werbin, Biochim. Biophys. Acta 1072, 1 (1991); V. J. Cristofalo and R. J. Pignolo, Physiol. Rev. 73, 617 (1993).
    • (1993) Physiol. Rev. , vol.73 , pp. 617
    • Cristofalo, V.J.1    Pignolo, R.J.2
  • 34
  • 35
    • 0028564951 scopus 로고
    • Telomerase activity was assayed by the telomere repeat amplification protocol [N. W. Kim, et al., Science 266, 2011 (1994)].
    • (1994) Science , vol.266 , pp. 2011
    • Kim, N.W.1
  • 36
    • 1842365612 scopus 로고    scopus 로고
    • unpublished observations
    • W. Wei and J. M. Sedivy, unpublished observations.
    • Wei, W.1    Sedivy, J.M.2
  • 39
    • 0026648674 scopus 로고
    • Apoptotic cell death was demonstrated by flow cytometry (75), in situ DNA end labeling [Y. Gavrieli, Y. Sherman, A. A. Ben-Sasson, J. Cell Biol. 119, 493 (1992)], and nucleosomal laddering (24).
    • (1992) J. Cell Biol. , vol.119 , pp. 493
    • Gavrieli, Y.1    Sherman, Y.2    Ben-Sasson, A.A.3
  • 41
    • 1842406739 scopus 로고    scopus 로고
    • note
    • We thank R. Halaban for establishing cell strains, H. Ozer and J. McCormick for advice on HDF culture, B. Vogelstein for the hyg p21 vector, and H.-F. Mark for karyotype analysis. Supported by grants from the Yale Skin Disease Research Center and the American Anti-vivisection Society (J.P.B.) and by NIH grant GM41690 (J.M.S).


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