The Molecular Structure of Green Fluorescent Protein

Nature Biotechnology

Volumn 14, Issue 10, 1996, Pages 1246-1251

  Yang, Fan ^a   Moss, Larry G ^b   Phillips, George N ^a  

^a RICE UNIVERSITY   (United States)

^b TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIMER; FLUORESCENT DYE; GLYCINE; MUTANT PROTEIN; PROTEIN; SERINE; TYROSINE;

ALPHA HELIX; ARTICLE; CRYSTAL STRUCTURE; CYCLIZATION; FLUORESCENCE; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN FOLDING; PROTEIN STRUCTURE; SPECTROSCOPY; X RAY DIFFRACTION;

ANIMALS; BIOTECHNOLOGY; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MODELS, MOLECULAR; MUTATION; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY;

EID: 0029780351     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt1096-1246     Document Type: Article

References (46)

1. Morin, J. and Hastings, J. 1971. Energy transfer in a bioluminescent system, J. Cell Physiol. 77:313-318.
2. Ward, W. 1979. pp. 1-57 in Photochemical and photobiological reviews. K. Smith (ed.). Plenum Press, New York.
3. Prasher, D., Eckenrode, V, Ward, W., Prendergast, F., and Cormier, M. 1992. Primary structure of Die Aequorea victoria green-fluorescent protein. Gene 111:229-233.
4. Chalfie, M., Tu, Y., Euskirchen, G., Ward, W., and Prasher, D. 1994. Green fluorescent protein as a marker for gene expression. Science 263:802-805.
5. Kahana, J., Schapp, B., and Silver, P. 1995. Kinetics of spindle pole body separation in budding yeast. Proc. Natl. Acad. Sci. USA 92:9707-9711.
6. Moores, S., Sabry, J., and Spudich, J. 1996. Myosin dynamics in live Dictyostelium cells. Proc. Natl. Acad. Sci. USA 93:443-446.
7. Casper, S. and Holt, C. 1996. Expression of the green fluorescent protein-encoding gene from a tobacco mosaic virus-based vector. Gene 173:69-7
8. Epel, B., Padgett, H., Heinlein, M., and Beachy, R. 1996. Plant virus movement protein dynamics probed with a GFP-protein fusion. Gene 173:75-79.
9. Wang, S. and Hazelrigg, T. 1994. Implications for bed mRNA localization from spatial distribution of exu protein In Drosophila oogenesis. Nature 369:400-403.
10. Amsterdam, A., Lin, S., Moss, L, and Hopkins, N. 1996. Requirements for green fluorescent protein detection in transgenic zebrafish embryos. Gene 173:99-103.
11. Ludin, B., Doll, T., Meill, R., Kaech, S., and Matus, A. 1996. Application of novel vectors for GFP-tagging of proteins to study microtubule associated proteins. Gene 173:107-111.
12. DeGiorgi, E, Brini, M., Bastianutto, C, Marsault, R., Montera, M, Pizzo, R, et al. 1996. Tatgeting aequorin and green fluorescent protein to intracellular organelles. Gene 173:113-117.
13. Cubiti, A, Heim, R., Adams, S., Boyd, A, Gross, L, and Tsien, R. 1995. Understanding, improving and using green fluorescent proteins. TIBS 20:448-455.
14. Olsen, K, McIntosh, J., and Olmstead, J. 1995. Analysis of MAP4 function in living cells using green fluorescent protein (GFP) chimeras. J. Cell Biol. 130:639-650.
15. Rizzuto, R., Brini, M., De Giorgi, F., Rossi, R., Heim, R., Tsien, R., et al. 1996. Double labeling of subcellular structures with organelle-targeted GFP mutants in vivo. Curr. Biol. 6:183-188.
16. Kaether, C. and Gerdes, H. 1995. Visualization of protein transport along the secretory pathway using green fluorescent protein. FEBS Lett 369:267-271.
17. Marshall, J., Molloy, R., Moss, G., Howe, J., and Hughes, T. 1995. The jellyfish green fluorescent protein: a new tool for studying ion channel expression and function. Neuron 14:211-215.
18. Mitra, R., Silva, C, and Youvan, D. 1996. Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein. Gene 173:13-7.
19. Kahana, J. and Silver, P. 1996. pp. 9.7.22-9.7-28. in Current protocols in molecular biology. Ausabel F., et al. (eds.). Green and Wiley, New York.
20. Cody, C.W., Prasher, D.C., Westler, W.M., Prendergast, F.G., and Ward, W.W 1993. Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein. Biochemistry. 32:1212-1218.
21. Heim, R., Prasher, D.C., and Tsien, R.Y. 1994. Wavelength mutations and post-translational autoxidation of green fluorescent protein. Proc. Natl. Acad. Sci. USA 91:12501-12504.
22. Delagrave, S., Hawtin, R., Silva, C, Yang, M., and Youvan, D. 1995. Red-shifted excitation mutants of the green fluorescent protein. Bio/Technology 13:151-154.
23. Lim, C, Kimata, K., Oka, M., Nomaguchi, K., and Kohno, K., 1995. Thermo-sensitivity of a green fluorescent protein utilized to reveal novel nuclear-like compartments. J. Biochem. (Tokyo) 118:13-17.
24. Ward, W.W. and Bokman, S.H. 1982. Reversible denaturation of Aequorea green-fluorescent protein: physical separation and characterization of the renatured protein. Biochemistry 21:4535-4540.
25. Ward, W, Prentice, H., Roth, A., Cody, C. and Reeves, S. 1982. Spectral perturbations of the Aequoria green fluorescent protein. Photochem. Photobiol. 35:803-808.
26. Inouye, S. and Tsuji, F.I. 1994. Evidence for redox forms of the Aequorea green fluorescent protein. FEBS Lett. 351:211-214.
27. Dopf, J. and Horiagan, T. 1996. Deletion mapping of the Aequoria victoria green fluorescent protein. Gene 173:39-44.
28. Heim, R., Cubitt, A., and Tsien, R. 1995. Improved green fluorescence. Nature 373:663-664.
29. Cormack, B., Valdivia, R., and Falkow, S. 1996. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173:33-38.
30. Ehrig, T., O’Kane, D., and Prendergast, F. 1995. Green-fluorescent protein mutants with altered fluorescence excitation spectra. FEBS Lett. 367:163-166.
31. Crameri, A, Whitehom, E., Tate, E., and Stemmer, W. 1996. Improved green fluorescent protein by molecular evolution using DNA shuffling. Nature Biotech. 14:315-319.
32. Perozzo, M., Ward, K., Thompson, R., and Ward, W. 1988. X-ray diffraction and time-resolved fluorescence analyses of Aequorea green fluorescent protein crystals. J. Biol. Chem. 263:7713-7716.
33. Merbs, S. and Nathans, J. 1992. Absorption spectra of the hybrid pigments responsible for anomalous color vision. Science 258:464-466.
34. Rao, B., Kemple, M., and Prendergast, F. 1980. Proton nuclear magnetic resonance and fluorescence spectroscopic studies of segmental mobility in aequorin and a green fluorescent protein from Aequorea forskalea. Biophys. J. 32:630-632.
35. Ormö, M., Cubitt, A., Kallio, K., Gross, L, Tsien, R., and Remington, S. 1996. Crystal structure of the Aequorea victoria green fluorescent protein. Science In press.
36. Wright, H. 1991 Nonenzymatic deamidation of asparaginyl and glutaminyl residues in proteins. Crit. Rev. Biochem. Mol. Biol. 26:1-52.
37. Chattoraj, M., King, B., Bublitz, G., and Boxer, S. 1996. Ultra-fast excited state dynamics in green fluorescent protein: Multiple states and proton transfer. Proc. Natl. Acad. Sci. USA 93:8362-8367.
38. Otwinowski, Z. 1993. Data collection and processing, in Proceedings of the CCP4 study weekend. Warrington, England: Daresbury Laboratory.
39. Sheldrick, G., Dauter, Z., Wilson, K., Hope, H., and Sieker, L. 1993. The application of direct methods and Patterson interpretation to high-resolution native protein data. Acta Cryst. D49:18-23.
40. Terwilliger, T., Kim, S.-H., and Eisenberg, D. 1987. Generalized method of determining heavy-atom positions using the difference Patterson function. Acta Cryst. A43:1-5.
41. Yang, W., Hendrickson, W., Crouch, R., and Satow, Y 1990. Structure of ribonuclease H phased at 2 A by MAD analysis of the seleniomethionyl protein. Science 249:1398-1405.
42. Collaborative Computational Project, N. 1994. The CCP4 suite: Programs for protein crystallography. Acta Cryst D50:760-763.
43. Jones, T, Zou, J., Cowan, S., and Kjeldgaard, M. 1991. Improved methods for building protein models in electron density maps and the location of errors in these models. Acta Crystallogr. 47:110-119.
44. Brunger, A. 1992. X-PLOR Version 3.1: A system for X-ray crystallography and NMR. Yale University Press, New Haven, CT.
45. Carson, M. 1987. Ribbon models of macromolecules. J. Mol. Graphics 5:103-106.
46. Sayle, R. and Milner-White, E. 1995. RasMol: Biomolecular graphics for all. TIBS 20:374-375.