Overview of expression of hepatitis B surface antigen in transgenic plants

Vaccine

Volumn 28, Issue 46, 2010, Pages 7351-7362

  Guan, Zheng jun ^a,b   Guo, Bin ^a   Huo, Yan lin ^b   Guan, Zheng ping ^c   Wei, Ya hui ^a  

^a BEIJING UNIVERSITY OF POSTS AND TELECOMMUNICATIONS   (China)

^b YUNCHENG UNIVERSITY   (China)

^c NANJING AGRICULTURAL UNIVERSITY   (China)

Author keywords

Expression; HBsAg; HBV; Transgenic plant

Indexed keywords

HEPATITIS B SURFACE ANTIGEN; HEPATITIS B VACCINE;

ANTIGEN EXPRESSION; BANANA; EXPRESSION VECTOR; GENE EXPRESSION; IMMUNOGENICITY; MOLECULAR BIOLOGY; NONHUMAN; POTATO; PRIORITY JOURNAL; REVIEW; TOBACCO; TOMATO; TRANSGENIC PLANT;

ANIMALS; GENETIC VECTORS; HEPATITIS B SURFACE ANTIGENS; HEPATITIS B VACCINES; HUMANS; PLANTS, GENETICALLY MODIFIED;

EID: 77958155381     PISSN: 0264410X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vaccine.2010.08.100     Document Type: Review

References (118)

1. Michel M.L. Towards immunotherapy for chronichepatitis B virus infections. Vaccine 2002, 20:83-88.
2. Ganem D., Schneider R.J. Hepadnaviridae: the viruses and their replication. Fields Virol 2001, 4:2923-2969.
3. Heermann K.H., Goldmann U., Schwartz W., Seyffarth T., Baumgarten H., Gerlich W.H. Large surface proteins of hepatitis B virus containing the pre-S sequence. J Virol 1984, 52:396-402.
4. Yang HJ. The exploratory study on genetically engineered therapeutic hepatitis B vaccine. Dissertation for Master Degree of Xiamen University 2002; 7-10.
5. Streatfield S.J., Howard J.A. Plant-based vaccines. Int J Parasitol 2003, 33:479-493.
6. Dane D.S., Cameron C.H., Briggs M. Virus-like particles in serum of patients with Australia-antigen-associated hepatitis. Lancet 1970, 295:695-698.
7. Lau J.Y., Wright T.L. Molecular virology and patogenesis of hepatitis B. Lancet 1993, 342:1335-1340.
8. Yang D.L., Ye S.Y. Immunology of infectious diseases 1998, Hubei Science and Technology Publish House, p. 104-15.
9. Tiollais P., Christine P., Dejean A. The hepatitis B virus. Nature 1985, 371:489.
10. Levrero M., Balsano C., Avantaggiati M.L. Hepatits B virus and hepatocellular carcinoma: a possible role for the viral transactivators. Ital J Gastroenterol 1991, 23:576-583.
11. Luo K.X. HBV-basic research and clinic (1st version) 1997, People's Medical Publishing House, Beijing, p. 13-16.
12. Michel M.L., Davis H.L., Schleef M. DNA-mediated immunization to the hepatitis B surface antigen in mice: aspects of humoral response mimic hepatitis B viral infection in humans. Proc Natl Acad Sci USA 1995, 92:5307-5311.
13. d mice after DNA immunization of HBV core gene. Chin J Hepatol 1999, 7:107-109.
14. Davis L., Michel M.L., Whalen G. DNA-based immunization induces continuous secretion of hepatitis B surface antigen and high levels of circulating antibody. Hum Mol Genet 1993, 2:1847-1849.
15. Tacket C.O., Roy M.J., Widera G. Phase I safety and immune response studies of a DNA vaccine encoding hepatitis B surface antigen delivered by a gene delivery device. Vaccine 1999, 17:2826-2828.
16. Tiollais P., Couillin I., Mancini M. Specific vaccine therapy in chronic hepatitis B and DNA immunization against hepatiis B. Gastroenterol Hepatol 1999, 14(A):257-259.
17. Roy M.J., Wu M.S., Barr L.J. Induction of antigen-specific CD8+ T cells. T helper cells, and protective levels of antibody in human by particle-mediated administration of a hepatitis B virus DNA vaccine. Vaccine 2001, 19:764-778.
18. Deng T., Wu S.S., Chen T.B., Zhang X.H., Chen N.L., Jia K.M. The immune response in Balb/c mice immunized with hepatitis B virus core DNA-based vaccine. Chin J Infect Dis 1998, 16:216-218.
19. Ye J., Ren H., Gu B.Y., Tao X.H. Study on six kinds of eukaryotic expression plasmid as HBV-DNA vaccine in mice. Chin J Microbiol Immunol 2000, 20:369-370.
20. Loirat D., Li Z., Mancini M. Muscle specific expression of HBsAg: no effect on DNA-raised immune response. Virology 1999, 260:74-83.
21. Yuan Z.H., Fang X., Zheng L.J., Yao Y., He L.F., Wen Y.M. HBV DNA vaccine immunization and the effects of DNA vector constructs and routes of gene delivery on the induction of antiviral immunity. Chin J Microbiol Immunol 1999, 19:25-28.
22. Gleba Y., Klimyuk V., Marillonnet S. Viral vectors for the expression of proteins in plants. Curr Opin Biotechnol 2007, 18:134-141.
23. Daniell H., Kumar S., Dufourmantel N. Breakthrough in chloroplast genetic engineering of agronomically important crops. Trends Biotechnol 2005, 23:238-245.
24. Fischer R., Stoger E., Schillberg S., Christou P., Twyman R.M. Plant-based production of biopharmaceuticals. Curr Opin Plant Biol 2004, 7:152-158.
25. Mason H.S., Lam D.M.K., Arntzen C.J. Expression of hepatitis B surface antigen in transgenic plants. Proc Natl Acad Sci USA 1992, 89:11745-11749.
26. Liu Y.L., Wang J.F., Qiu B.S., Zhao S.Z., Tian B. Expression of human hepatitis B virus surface antigen gene in transgenic tobacco. Sci China B 1993, 23:252-255.
27. Thanavala Y., Yang Y.F., Lyons P. Immunogenicity of transgenic plant-derived hepatitis B surface antigen. Proc Natl Acad Sci USA 1995, 92:3358-3361.
28. Huang Z., Elkin G., Maloney B.J. Virus-like particle expression and assembly in plants: hepatitis B and Norwalk viruses. Vaccine 2005, 23:1851-1858.
29. Ramjrez N., Lorenzo D., Palenzuela D., Herrera L., Ayala M., Fuentes A., et al. Single-chain antibody fragments specific to the hepatitis B surface antigen, produced in recombinant tobacco cell cultures. Biotechnol Lett 2000, 22:1233-1236.
30. Sojikul P., Buehner N., Mason H.S. A plant signal peptide-hepatitis B surface antigen fusion protein with enhanced stability and immunogenicity expressed in plant cells. Proc Natl Acad Sci USA 2003, 100:2209-2214.
31. He Z.M., Qi Y., Xu Y. Expression of human hepatitis B virus surface antigen middle protein gene in transgenic tobacco: potential for edible vaccines. Food Biotechnol 2006, 20:243-250.
32. Polkinghorne I., Hamerli D., Cowan P., Duckworth J. Plant-based immunocontraceptive control of wildlife-" potentials, limitations, and possums" Vaccine 2005, 23:1847-1850.
33. Domansky N., Ehsani P., Salmanian A.H., Medvedeva T. Organ-specific expression of hepatitis B surface antigen in potato. Biotechnol Lett 1995, 17:863-866.
34. Ehsani P., Khabiri A., Domansky N.N. Polypeptides of hepatitis B surface antigen produced in transgenic potato. Gene 1997, 190:107-111.
35. Richter L.J., Thanavala Y., Arntzen G.J., Mason H.S. Production of hepatitis B surface antigen in transgenic plants for oral immunization. Nat Biotechnol 2000, 18:1167-1171.
36. Kong Q.X., Richter L., Yang Y.F. Oral immunization with hepatitis B surface antigen expressed in transgenic plants. Proc Natl Acad Sci USA 2001, 98:11539-11544.
37. Smith M.L., Richter L.J., Arntzen C.J. Structural characterization of plant-derived hepatitis B surface antigen employed in oral immunization studies. Vaccine 2003, 21:4011-4021.
38. Shulga N.Y., Rukavtsova E.B., Krymsky M.A., Borisova V.N., Melnikov V.A., Bykov V.A., et al. Expression and characterization of hepatitis B surface antigen in transgenic potato plants. Biochemistry (Mosc) 2004, 69:1158-1164.
39. Zhao C.H., Wang R., Zhao C.S., Wang G.L., Tian P. Expression of human HBV surface antigen gene with and without preS in transgenic tomato. J Agric Biotechnol 2000, 8:85-88.
40. Ma Y., Lin S.Q., Gao Y., Zhang J., Lu L.X., Xia N.S. Transformation of HBsAg (hepatitis B virus surface antigen) into tomato plants. J Fujian Agric Forest Univ (Nat Sci Ed) 2002, 31:223-228.
41. Carolina C., Francisco A.C. Tomato transformation and transgenic plant production. Plant Cell Tissue Organ Cult 2004, 76:269-275.
42. Wang Y.Q., Li T. Transformation of HBsAg gene into tomato and production of transgenic tomato plants. J Southwest Univ (Nat Sci Ed) 2008, 30:78-83.
43. Hao H.Y., Wei Y.H., Zhu J.G. Expression of oral hepatitis B vaccine in transgenic tomato. Food Sci 2007, 28:201-204.
44. Salyaev R.K., Rekoslavskaya N.I., Stolbikov A.S., Hammond R.W., Shchelkunov S.N. Synthesis of hepatitis B virus surface antigen in tomato plants transgenic for the preS2-S gene. Doklady Biochem Biophys 2007, 416:290-293.
45. Srinivas L., Sunil-Kumar G.B., Ganapathi T.R., Revathi C.J., Bapat V.A. Transient and stable expression of hepatitis B surface antigen in tomato (Lycopersicon esculentum L.). Plant Biotechnol Rep 2008, 2:1-6.
46. Mason H.S., Warzecha H., Mor T., Arntzen C.J. Edible plant vaccines: applications for prophylactic and therapeutic molecular medicine. Trends Mol Med 2002, 8:324-329.
47. Trivedi P.K., Nath P. MaExp1, an ethylene-induced expansin from ripening banana fruit. Plant Sci 2004, 167:1351-1358.
48. Hood E.E., Jilka J.M. Plant-based production of xenogenic proteins. Curr Opin Biotechnol 1999, 10:382-386.
49. Sunil-Kumar G.B., Ganapathi T.R., Revathi C.J., Srinivas L., Bapat V.A. Expression of hepatitis B surface antigen in transgenic banana plants. Planta 2005, 222:484-493.
50. Hu W., Xu B.Y., Zhang J.B., Yang X.Y., Li M.Y., Ning W.B., et al. Construction of vector for expression of HBsAg gene driven by a banana fruit specific promoter. J Fruit Sci 2008, 25:691-694.
51. Elkholy S.F., Ismail R.M., Bahieldin A., Sadik A.S., Madkour M.A. Expression of hepatitis B surface antigen (HBsAg) gene in transgenic banana (Musa Sp.). Arab J Biotechnol 2009, 12:291-302.
52. Smith M.L., Mason H.S., Shuler M.L. Hepatitis surface (HBsAg) expression in plant cell culture: kinetics of antigen accumulation in batch culture and its intracellular form. Biotechnol Bioeng 2002, 80:812-822.
53. Muller C.P., Fack F., Damien B., Bouche F.B. Immunogenic measles antigens expressed in plants: role as an edible vaccine for adults. Vaccine 2003, 23:816-819.
54. Zhao X.Y., Zhang X.D., Zhang T., Yan H.P., Luo C.X., Yang F.P. Cloning and expression of the hepatitis B surface antigen in carrots. Prog Microbiol Immunol 2002, 30:1-4.
55. Imani J., Berting A., Nitsche S., Schaefer S., Gerlich W.H., Neumann K.H. The integration of a major hepatitis B virus gene into cell-cycle synchronized carrot cell suspension cultures and its expression in regenerated carrot plants. Plant Cell Tissue Organ Cult 2002, 71:157-164.
56. Chen H.Y., Zhang J., Gao Y., Du H.L., Ma Y., Zheng W.Z., et al. Transforming HBsAg into peanut and detection of its immunogenicity. Lett Biotechnol 2002, 13:245-251.
57. Zhang R., Wei Y.H., Liu K. Construction on plant expression vector with hepatitis B surface antigen gene. J Northwest Univ (Sci Online) 2005, 3:1-6.
58. Zhu J.G., Wei Y.H., Guo Z.G. The transformation and expression of HBsAg gene in Arachis hypogaea. Chin Bull Bot 2006, 23:665-669.
59. Jiang P., Qin S., Tseng C.K. Expression of hepatitis B surface antigen gene (HBsAg) in Laminaria japonica (Laminariales Phaeophyta). Chin Sci Bull 2002, 47:1438-1440.
60. Gao Y., Ma Y., Li M., Cheng T., Li S.W., Zhang J., et al. Oral immunization of animals with transgenic cherry tomatillo expressing HBsAg. World J Gastroenterol 2003, 9:996-1002.
61. Pniewski T., Kapusta J., Plucienniczak A. Agrobacterium-mediated transformation of yellow lupin to generate callus tissue producing HBV surface antigen in a long-term culture. J Appl Genet 2006, 47:309-318.
62. Lou X.M., Zhang Z., Yao H.Q., Xiong A.S., Wang H.K., Peng R.H., et al. Expression of human hepatitis B virus large surface antigen gene PRS-S1S2S in transgenic apples. J Fruit Sci 2005, 22:601-605.
63. He H.X., Lin C.G., Wang M., Dong Y.S. Expression vectorion construction of hepatitis B surface antigen (HBsAg) gene and transformation to Crowtoe (Lotus corniculatus). J Agric Biotechnol 2007, 15:115-118.
64. Marcondes J., Hansen E. Transgenic lettuce seedlings carrying hepatitis B virus antigen HBsAg. Braz J Infect Dis 2008, 12:469-471.
65. Fan J.M., Zhang Q., Xu J., Zhu S., Ke T., Gao D.F., et al. Inhibition on hepatitis B virus in vitro of recombinant MAP30 from bitter melon. Mol Biol Rep 2009, 36:381-388.
66. Kapusta J., Modelska A., Figlerowicz M. A plant-derived edible vaccine against hepatitis B virus. FASEB J 1999, 13:1796-1799.
67. Sunil-Kumar G.B., Ganapathi T.R., Revathi C.J., Prasad K.S.N., Bapat V.A. Expression of hepatitis B surface antigen in tobacco cell suspension culture. Protein Expr Purif 2003, 32:10-17.
68. Smith G.L., Mackett M., Moss B. Infectious vaccinia virus recombinants that express hepatitis B virus surface antigen. Nature 1983, 302:490-495.
69. Yusibov V., Modelska A., Steplewski K. Antigens produced in plants by infection with chimeric plant viruses immunize against rabies virus and HIV-1. Proc Natl Acad Sci USA 1997, 94:5784-5788.
70. Koprowski H., Yusibov V. The green revolution: plants as heterologousexpression vectors. Vaccine 2001, 19:2735-2741.
71. Perez Filgueira D.M., Zamorano P.I., Dominguez M.G., Taboga O., Del MZajac M.P., Puntel M., et al. Bovine herpes virus gDprotein produced in plants using a recombinant tobacco mosaic virus (TMV) vector possesses authentic antigenicity. Vaccine 2003, 21:4201-4209.
72. Kang T.J., Kang K.H., Kim J.A., Kwon T.H., Jang Y.S., Yang M.S. High-level expression of the neutralizing epitope of porcine epidemic diarrhea virus by a tobacco mosaic virus-based vector. Protein Expr Purif 2004, 38:129-135.
73. AikSeng O., SiangHee T., Rosmawati M., Noorsaadah A.R., Rofina Y.O. The full-length clone of cucumber green mottle mosaic virus and its application as an expression system for hepatitis B surface antigen. J Biotechnol 2006, 121:471-481.
74. Meilyn R., Nadia I.R., Walter G., Alejandro F., Marlene P., Marta A., et al. Transient expression of a full-size antibody against hepatitis B surface antigen in plant cell suspension cultures. Biotecnología Aplicada 2003, 20:152-154.
75. Yang F.P., Wang H.Z., Chen X.Q., Zhang L.Q., Zhang X.D. Transient expression of recombinant HBsAg gene from virus X vector in Nicotiana benthamiana. Acta Bot Boreal Occident Sin 2008, 28(2):217-222.
76. Marillonnet S., Giritch A., Gils M., Kandzia R., Klimyuk V., Gleba Y. In planta engineering of viral RNA replicons: efficient assembly by recombination of DNA modules delivered by Agrobacterium. Proc Natl Acad Sci USA 2004, 101:6852-6857.
77. Huang Z., LePore K., Elkin G., Thanaval Y., Mason H.S. High-yield rapid production of hepatitis B surface antigen in plant leaf by a viral expression system. Plant Biotechnol J 2008, 6:202-209.
78. Huang Z., Santi L., LePore K., Kilbourne J., Arntzen C.J., Mason H.S. Rapid, high-level production of hepatitis B core antigen in plant leaf and its immunogenicity in mice. Vaccine 2006, 24:2506-2513.
79. Kim S.R., Kim Y., An G. Identification of methyl Jasmonate and salicylic acid response elements from the nopaline synthase (nos) promoter. Plant Physiol 1993, 103:97-103.
80. Qin X.F., Holulgue L., Horvath D.M., Chue N.H. Immediate early transcription by salicylic acid via the cauliflower mosaic virus as-1 element. Plant Cell 1994, 6:863-874.
81. Borisjuk N.V., Borisjuk L.G., Logendra S., Petersen F., Gleba Y., Raskin I. Production of recombinant proteins in plant root exudates. Nat Biotechnol 1999, 17:466-469.
82. Komarnytsky S., Borisjuk N.V., Borisjuk L.G., Alam M.Z., Raskin I. Production of recombinant proteins in tobacco guttation fluid. Plant Physiol 2000, 124:927-933.
83. Sunil-Kumar G.B., Srinivas L., Ganapathi T.R., Bapat V.A. Hepatitis B surface antigen expression in transgenic tobacco (Nicotiana tabacum) plants using four different expression cassettes. Plant Cell Tissue Organ Cult 2006, 84:315-323.
84. Milich D.R., McLachlan A. The nucleocapsid of hepatitis B virus is both a T-cell-independent and a T-cell-dependent antigen. Science 1986, 234(4782):1398-1401.
85. Milich D.R., Chen M., Schodel F., Peterson D.L., Jones J.E., Hughes J.L. Role of B cells in antigen presentation of the hepatitis B core. Proc Natl Acad Sci USA 1997, 94(26):14648-14653.
86. Francis M.J., Hastings G.Z., Brown A.L., Grace K.G., Rowlands D.J., Brown F., et al. Immunological properties of hepatitis B core antigen fusion proteins. Proc Natl Acad Sci USA 1990, 87:2545-2549.
87. Milich D.R., Chen J.L., Hughes J.E. The secreted hepatitis B precore antigen can modulate the immune response to the nucleocapsid: a mechanism for persistence. J Immunol 1998, 160:2013-2021.
88. Milich D.R. Immune response to the hepatitis B virus: infection, animal models, vaccine. Viral Hepat Rev 1997, 3:63-103.
89. Tsuda S., Yoshioka K., Tanaka T. Application of the humanhepatitis B virus core antigen from transgenic tobacco plants for serological diagnosis. Vox Sang 1998, 74(3):148-155.
90. Gergana Z., Stefan D., Ivan M., Anton A. Molecular farming-the production of recombinant submit hepatitis B vaccine in tobacco plants. Proceedings of the Balkan scientific conference of biology in plovdiv (Bulgaria) 2005, 329-338.
91. Mechtcheriakova I.A., Eldarov M.A., Nicholson L., Shanks M., Skryabin K.G., Lomonossoff G.P. The use of viral vectors to produce hepatitis B virus core particles in plants. J Virol Methods 2006, 131:10-15.
92. Chen H.S., Kaneko S., Girones R. The woodchuck hepatitis virus X gene is important for establishment of virus infection in woodchucks. J Virol 1993, 67:1218-1226.
93. Rossner M.T. Hepatitis B virus X-gene product: apromiscuous transcriptional activator. J Med Virol 1992, 36:101-117.
94. Norton P.A., Reis H.M., Prince S., Larkin J., Pan J., Liu J., et al. Activation of fibronectin gene expression by hepatitis B virus x antigen. J Viral Hepat 2004, 11:332-341.
95. Wang W., London W.T., Lega L., Feitelson M.A. Hepatitis B×antigen in liver from carrier patients with chronic hepatitis and cirrhosis. Hepatology 1991, 14:29-37.
96. Jin Y.M., Yun C., Park C., Wang H.J., Cho H. Expression of hepatitis B virus X protein is closely correlated with the high periportal inflammatory activity of liver diseases. J Viral Hepat 2001, 8:322-330.
97. Katayama K., Hayashi N., Sasaki Y., Kasahara A., Ueda K., Fusamoto H. Detection of hepatitis B virus X gene protein and antibody in type B chronic liver disease. Gastroenterology 1989, 97:990-998.
98. Ou J.H. Molecular biology of hepatitis B virus e antigen. J Gastroenterol Hepatol 1997, 12:178-187.
99. Miyakawa Y., Okamoto H., Mayumi M. The molecular basis of hepatitis B e antigen (HBeAg) negative infections. J Viral Hepat 1997, 4:1-8.
100. Milich D.R., Johns J.E., Hyghes J., Pricer A., Raney K., Mclachlan A. Is a function of the secreted hepatitis B e antigen to induced immunologic tolerance in utero. Proc Natl Acad Sci USA 1990, 87:6599-6603.
101. Huang R.L., Huang Y.L., Ou J.C., Chen C.C., Hsu F.L., Chang C.M. Screening of 25 compounds isolated from Phyllanthus species for anti-human hepatitis B virus in vitro. Phytother Res 2003, 17:449-453.
102. Wang Y.J., Li G.Q., Liu D.H. Transgenic plants as edible HBV vaccine production systems. Lett Biotechnol 1997, 5:21-25.
103. Min S.S., Eun H.K., Young I.L. A flavonoid from medicinal plants blocks hepatitis B virus-e antigen secretion in HBV-infected hepatocytes. Antiviral Res 2005, 67:163-168.
104. Thanavala Y., Yang Y.F., Lyons P., Mason H.S., Arntzen C. Immunogenicity of transgenic plant-derived hepatitis B surface antigen. Proc Natl Acad Sci USA 1995, 92:3358-3361.
105. Thanavala Y., Mahoney M., Pal S. Immunogenicity in humans of an edible vaccine for hepatitis B. Proc Natl Acad Sci USA 2005, 102:3378-3382.
106. Li Y., Zhang Q.B., Wu X.H. Construction of recombinant vector which express hepatitis B surface antigen in tobacco and its transferation. Acta Agriculturae Borealioccidentalis Sinica 2001, 10:41-43.
107. Sunil-Kumar G.B., Ganapathi T.R., Srinivas L., Revathi C.J., Bapat V.A. Hepatitis B surface antigen expression in NT-1 cells of tobacco using different expression cassettes. Biol Plantarum 2007, 51(3):467-471.
108. Rukavtsova E.B., Gayazova A.R., Chebotareva E.N., Buryanov Y.I. Production of marker-free plants expressing the gene of the hepatitis B virus surface antigen. Russ J Genet 2009, 45:924-928.
109. Li T., Wang Y.Q., Lu Z.H. Transformation of HBsAg gene into tobacco mediated by Agrobacterium tumefaciens. Bull Bot Res 2009, 29:460-465.
110. Joung Y.H., Youm J.W., Jeon J.H., Lee B.C., Ryu C.J., Hong H.J., et al. Expression of the hepatitis B surface S and preS2 antigens in tubers of Solanum tuberosum. Plant Cell Rep 2004, 22:925-930.
111. Lou X.M., Yao H.Q., Zhang Z., Peng R.H., Xiong A.S., Wang H.K. Expression of the human hepatitis B virus large surface antigen gene in transgenic tomato plants. Clin Vaccine Immunol 2007, 14:464-469.
112. Deineko E.V., Zagorskaya A.A., Pozdnyakov S.G., Filipenko E.A., Permyakova N.V., Sidorchuk Y.V., et al. Comparative analysis of HBV M-antigen production in leaves of individual transgenic carrot plants. Doklady Biochem Biophys 2009, 425:76-79.
113. Yao WM. Expression of hepatitis B surface antigen in ginseng cell. Dissertation for Master Degree of Jilin University 2005; 27-32.
114. Ooi A., Tan S., Mohamed R., Rahman N.A., Rofina-Othman Y. The full-length clone of cucumber green mottle mosaic virus and its application as an expression system for hepatitis B surface antigen. J Biotechnol 2006, 121:471-481.
115. He Z.M., Jiang X.L., Qi Y., Luo D.Q. Assessment of the utility of the tomato fruit-specific E8 promoter for driving vaccine antigen expression. Genetica 2008, 133:207-214.
116. Clendennen S.K., Lopez G.R., Gomez L.M. The abundant 31-kilodalton banana pulp protein is homologous to class-III acidic chitinases. Phytochemistry 1998, 47:613-619.
117. Lauterslager T.G., Florack D.E., Wal T.J. Oral immunization of naïve and primed animals with transgenic potato tubers expression LT-B. Vaccine 2001, 19:2749-2755.
118. Chargelegue D., Obregon P., Drake P.M.W. Transgenic plants for vaccine production: expectations and limitation. Plants Trends 2001, 6:495-496.