Molecular biology of hepatitis B virus e antigen

Journal of Gastroenterology and Hepatology (Australia)

Volumn 12, Issue 9-10, 1997, Pages

  Ou, Jing Hsiung ^a  

^a UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

Author keywords

HBeAg biogenesis; Hepatitis B virus; Viral variants; Vital gene expression

Indexed keywords

HEPATITIS B(E) ANTIGEN;

ANTIGEN EXPRESSION; BIOGENESIS; CONFERENCE PAPER; GENE MUTATION; HEPATITIS B; HEPATITIS B VIRUS; HUMAN; MOLECULAR BIOLOGY; NONHUMAN; PRIORITY JOURNAL; RNA TRANSCRIPTION; VIRUS GENOME; VIRUS REPLICATION;

EID: 0030782534     PISSN: 08159319     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1440-1746.1997.tb00499.x     Document Type: Conference Paper

References (109)

1. Cimons M. White House to expand response to infectious diseases. ASM News 1996; 62: 450-3.
2. Ganem D. Hepadnaviridae: The viruses and their replication. In: Fields BN, Knipe DM, Howley PM, eds. Fields Virology, 3rd edn. Philadelphia: Lippincott-Raven, 1995; 2703-37.
3. Salfeld J, Pfaff E, Noah M, Schaller H. Antigenic determinants and functional domains in core antigen and e antigen from hepatitis B virus. J. Viral. 1989; 63: 798-808.
4. Magnius LO, Espmark JA. New specificities in Australia antigen positive sera distinct from the Le Bouvier determinants. J. Immunol. 1972; 109: 1017-21.
5. Ou JH, Laub O, Rutter WJ. Hepatitis B virus gene functions: The precore region targets the core antigen to cellular membranes and causes the secretion of the e antigen. Proc. Natl Acad. Sci. USA 1986; 83: 1578-82.
6. Echkardt SG, Milich DR, McLachlan A. Hepatitis B virus core protein has two nuclear localization sequences in the arginine-rich carboxyl terminus. J. Virol. 1991; 65: 575-82.
7. Yeh CT, Wong SW, Fung YK, Ou JH. Cell cycle regulation of nuclear localization of hepatitis B virus core protein. Proc. Natl Acad. Sci. USA 1993; 90: 6459-63.
8. Liao W, Ou JH. Phosphorylation and nuclear localization of the hepatitis B virus core protein: significance of serine in the three repeated SPRRR motifs. J. Virol. 1995; 69: 1025-9.
9. Guidotti LG, Martinez V, Loh YT, Regler CE, Chisari FV. Hepatitis B virus nucleocapsid particles do not cross the hepatocyte nuclear membrane in transgenic mice. J. Virol. 1994; 60: 5469-75.
10. Roossnick MJ, Jameel S, Loukin SH, Siddiqui A. Expression of hepatitis B viral core regions in mammalian cells. Mol. Cell. Biol. 1986; 6: 1391-400.
11. Garcia PD, Ou JH, Rutter WJ, Walter P. Targeting of the hepatitis B virus precore protein to the endoplasmic reticulum membrane: After signal peptide cleavage translocation can be aborted and the product released into the cytoplasm J. Cell Biol. 1988; 106: 1093-104.
12. Bruss V, Gerlich WH. Formation of transmembraneous hepatitis B e-antigen by cotranslational in vitro processing of the viral precore protein. Virology 1988; 163: 268-75.
13. Yang SQ, Walter M, Standring DN. Hepatitis B virus p25 precore protein accumulates in Xenopus oocytes as an untranslocated phosphoprotein with an uncleaved signal peptide. J. Virol. 1992; 66: 37-45.
14. Ou JH, Yeh CT, Yen TSB. Transport of hepatitis B virus precore protein into the nucleus after cleavage of its signal peptide. J. Virol. 1989; 63: 5238-43.
15. Yeh CT, Ou JH. Phosphorylation of hepatitis B virus precore and core proteins. J. Virol. 1991; 65: 2327-31.
16. Ou JH, Bell KD. Comparative studies of hepatitis B virus precore and core particles. Virology 1990; 174: 185-91.
17. Zhou S, Standring DN. Hepatitis B virus capsid particles are assembled from core-protein dimer precursors. Proc. Natl Acad. Sci. USA 1992; 89: 10 046-50.
18. Schodel F, Peterson D, Zheng J, Jones JE, Hughes JL, Milich D-R. Structure of hepatitis B virus core and eantigen: A single precore amino acid prevents nucleocapsid assembly. J. Biol. Chem. 1993; 268: 1332-7.
19. Schlicht HJ, Wasenauer G. The quaternary structure, antigenicity, and aggregational behavior of the secretory core protein of human hepatitis B virus are determined by its signal sequence. J. Viral. 1991; 65: 6817-25.
20. Zhou S, Standring DN. Cys residues of the hepatitis B virus capsid protein are not essential for the assembly of viral core particles but can influence their stability. J. Viral. 1992; 66: 5393-8.
21. Wasenauer G, Koch J, Schlicht HJ. A cysteine and a hydrophobic sequence in the noncleaved portion of the pre-C leader peptide determine the biophysical properties of the secretory core protein (HBe protein) of human hepatitis B virus. J. Virol. 1992; 66: 5338-46.
22. Jean-Jean O, Levrero M, Will H, Perricaudet M, Rossignol JM. Expression mechanism of the hepatitis B virus (HBV) C gene and biosynthesis of HBe antigen. Virology 1989; 170: 99-106.
23. Wang J, Lee AS, Ou JH. Proteolytic conversion of hepatitis B virus precore protein to e antigen end-product occurs in a post-endoplasmic reticulum compartment. J. Virol. 1991; 65: 5080-3.
24. Ou JH, Standring DN, Masiarz F, Rutter WJ. A signal peptide encoded within the precore region of hepatitis B virus directs the secretion of a heterogeneous population of e antigens in Xenopus oocytes. Proc. Natl Acad. Sci. USA 1988; 85: 8405-9.
25. Schlicht HJ, Schaller H. The secretory core protein of human hepatitis B virus is expressed on the cell surface. J. Virol. 1989; 63: 5399-404.
26. Takahashi K, Machida A, Funatsu G et al. Immunochemical structure of hepatitis Be antigen in the serum. J. Immunal. 1983; 130: 2903-7.
27. Wasenauer G, Koch J, Schlicht HJ. Relevance of cysteine residues for biosynthesis and antigenicity of human hepatitis B virus e antigen. J. Virol. 1993; 67: 1315-21.
28. Schlicht HJ. Biosynthesis of the secretory core protein of duck hepatitis B virus: Intracellular transport, proteolytic processing and membrane expression of the precore protein. J. Virol. 1991; 65: 3489-95.
29. Bertoletti A, Ferrari C, Fiaccadori F et al. HLA class I-restricted human cytotoxic T cells recognize endogenously synthesized hepatitis B virus nucleocapsid antigen. Proc. Natl Acad. Sci. USA 1991; 88: 10 445-9.
30. Chisari FV, Ferrai C. Hepatitis B virus immunopathogenesis. Annu. Rev. Immnnol. 1995; 13: 29-60.
31. Yuh CH, Chang YL, Ting LP. Transcriptional regulation of precore and pregenomic RNAs of hepatitis B virus. J. Virol. 1992; 66: 4073-84.
32. Zhang P, McLachlan A. Differentiation-specific transcriptional regulation of the hepatitis B virus nucleocapsid gene in human hepatoma cell lines. Virology 1994; 202: 430-40.
33. Yaginuma K, Koike K. Identification of a promoter region for 3.6-kilobase mRNA of hepatitis B virus and specific cellular binding protein. J. Virol. 1989; 63: 2914-20.
34. Honigawachs J, Faktor O, Dikstein R, Shaul Y, Laub O. Liver-specific expression of hepatitis B virus is determined by the combined action of the core gene promoter and the enhancer. J. Virol 1989; 63: 919-24.
35. Zheng YW, Yen TSB. Negative regulation of hepatitis B virus gene expression and replication by oxidative stress. J. Biol. Chem. 1994; 269: 8857-62.
36. Chen IH, Huang CJ, Ting LP. Overlapping initiator and TATA box functions in the basal core promoter of hepatitis B virus. J. Virol. 1995; 69: 3647-57.
37. Buckwold VE, Xu Z, Chen M, Yen TSB, Ou JH. Effects of a naturally occurring mutation in the hepatitis B virus basal core promoter on precore gene expression and viral replication. J. Virol. 1996; 70: 5845-51.
38. Yu X, Mertz JE. Promoters for synthesis of the pre-C and pregenomic mRNAs of human hepatitis B virus are genetically distinct and differentially regulated. J. Virol. 1996; 70: 8719-26.
39. Buckwold VE, Xu Z, Yen TSB, Ou JH. Effects of a frequent double-nucleotide basal core promoter mutation and its putative single-nucleotide precursor mutations on hepatitis B virus. J. Gen. Virol. 1997; (in press).
40. Baumert TF, Rogers SA, Hasegawa K, Liang TJ. Two mutations identified in a hepatitis B virus strain associated with fulminant hepatitis result in enhanced viral replication J. Clin. Invest. 1996; 98: 2268-76.
41. Yee JK. A liver-specific enhancer in the core promoter region of human hepatitis B virus. Science 1989; 246: 658-61.
42. Wang Y, Chen P, Wu X et al. A new enhancer element, ENII, identified in the X gene of hepatitis B virus. J. Virol. 1990; 64: 3977-81.
43. Yuh CH, Ting LP. The genome of hepatitis B virus contains a second enhancer: Cooperation of two elements within this enhancer is required for its function. J. Virol. 1990; 64: 4281-7.
44. Su H, Yee JK. Regulation of hepatitis B virus gene expression by its two enhancers. Proc. Natl Acad. Sci. USA 1992; 89: 2708-12.
45. Li M, Xie Y, Wu X, Kong Y, Wang Y. HNF3 binds and activates the second enhancer, ENII, of hepatitis B virus. Virology 1995; 214: 371-8.
46. Guo W, Chen M, Yen TSB, Ou JH. Hepatocyte-specific expression of the hepatitis B virus core promoter depends on both positive and negative regulation. Mol. Cell. Biol. 1993; 13: 443-8.
47. Lopez-Cabrera M, Letovsky J, Hu KQ, Siddiqui A. Transcriptional factor C/EBP binds to and transactivates the enhancer element II of the hepatitis B virus. Virology 1991; 183: 825-9.
48. Lopez-Cabrera M, Letovsky J, Hu KQ, Siddiqui A. Multiple liver-specific factors binds to the hepatitis B virus core/pregenomic promoter: Transactivations and repression by CCAAT/enhancer binding protein. Proc. Natl Acad. Sci. USA 1990; 87: 5069-73.
49. Yuh CH, Ting LP. C/EBP-like proteins binding to the functional box-alpha and box-beta of the second enhancer of hepatitis B virus. Mol Cell. Biol. 1991; 11: 5044-52.
50. Zhang P, Raney AK, McLachlan A. Characterization of functional Sp1 transcriptional factor binding site in the hepatitis B virus nucleocapsid promoter. J. Virol. 1993; 67: 1472-81.
51. Spandau DF, Lee CH. Repression of the hepatitis B virus enhancer by a cellular factor. J. Gen. Viral. 1992; 73: 131-7.
52. Gerlach KK, Schloemer RH. Hepatitis B virus C gene promoter is under negative regulation. Virology 1992; 189: 59-66.
53. Chen M, Ou JH. Cell type-dependent regulation of the activity of the negative regulatory element of the hepatitis B virus core promoter. Virology 1995; 214: 198-206.
54. Lo WY, Ting LP. Repression of enhancer II activity by a negative regulatory element in the hepatitis B virus genome. J. Virol. 1994; 68: 1758-64.
55. Buckwold VE, Chen M, Ou JH. Interaction of transcription factors RFX1 and MIBP1 with the γ motif of the negative regulatory element of the hepatitis B virus core promoter. Virology 1997; 227: 515-18.
56. Shaul Y, Rutter WJ, Laub O. A human hepatitis B viral enhancer element. EMBO J. 1985; 4: 427-30.
57. Hu KQ, Siddiqui A. Regulation of the hepatitis B virus gene expression by the enhancer element I. Virology 1991; 181: 721-6.
58. Guo W, Bell KD, Ou JH. Characterization of the hepatitis B virus EnhI enhancer and X promoter complex. J. Virol 1991; 65: 6686-92.
59. Chen M, Hieng S, Qian X, Costa R, Ou JH. Regulation of hepatitis B virus ENI enhancer activity by hepatocyte-enriched transcription factor HNF3. Virology 1994; 205: 127-32.
60. Ori A, Shaul Y. Hepatitis B virus enhancer binds and is activated by the hepatocyte nuclear factor 3. Virology 1995; 207: 98-106.
61. Kosovsky MJ, Huan B, Siddiqui A. Purification and properties of rat liver nuclear proteins that interact with the hepatitis B virus enhancer 1. J. Biol. Chem. 1996; 271: 21 859-69.
62. Garcia AD, Ostapchuk P, Hearing P. Functional interaction of nuclear factors EF-C, HNF-4, and RXR alpha with hepatitis B virus enhancer I. J. Virol. 1993; 67: 3940-50.
63. Huan B, Siddiqui A. Regulation of hepatitis B virus gene expression. J. Hepatol. 1993; 17 (Suppl. 3): S20-3
64. Carman WF, Jacyna MR, Hadziyannis S et al. Mutations preventing formation of hepatitis Be antigen in patients with chronic hepatitis B infection. Lancet 1989; ii: 588-90.
65. Brunetto MR, Giarin E, Oliveri F et al. Wild-type and e antigen-deficient hepatitis B viruses and course of chronic hepatitis. Proc. Natl Acad. Sci. USA 1991; 88: 4186-90.
66. Okamoto H, Yotsumoto S, Akahane Y et al. Hepatitis B viruses with precore region defects prevail in persistently infected hosts along with seroconversion to the antibody against e antigen. J. Virol. 1990; 64: 1298-303.
67. Omata M, Ehata T, Yokosuka O, Hosoda K, Ohto M. Mutations in the precore region of hepatitis B virus DNA in patients with fulminant and severe hepatitis N. Engl. J. Med. 1991; 324: 1699-704.
68. Carman W, Thomas H, Domingo E. Viral genetic variation: Hepatitis B virus as a clinical example. Lancet 1993; 341: 349-53.
69. Thomas HC. The emergence of envelope and precore/ core variants of hepatitis B virus: The potential role of antibody selection. J. Hepatol. 1995; 22 (Suppl. 1): 1-8.
70. Stephan W, Prince AM, Brotman B. Modulation of hepatitis B infection by intravenous application of an immunoglobulin preparation that contains antibodies to hepatitis Be and core antigens but not to hepatitis B surface antigen. J. Virol. 1984; 51: 420-4.
71. Murray K, Bruce S, Hinnen A et al. Hepatitis B virus antigens made in microbial cells immunize against viral infection. EMBO J. 1984; 3: 645-50.
72. Hawkins AE, Gilson RJC, Bickerton EA, Tedder RS, Weller ID. Conservation of precore and core sequences of hepatitis B virus in chronic viral carriers. J. Med. Virol. 1994; 43: 5-12.
73. Raimondo G, Meucci G, Sardo MA et al. Persistence of 'wild-type' and 'e-minus' hepatitis B virus infection in chronic healthy HBsAg/anti-HBe positive carriers. J. Hepatol. 1994; 20: 148-51.
74. Tong S, Li J, Vitvitski L, Trepo C. Active hepatitis B virus replication in the presence of anti-HBe is associated with viral variants containing an inactive pre-C region. Virology 1990; 176: 596-603.
75. Kim SK, Jang SK, Rho HM. Effects of frameshift mutation in the pre-C region of hepatitis B virus on the X and C genes. J. Gen. Virol. 1994; 75: 917 23.
76. Yuan TTT, Faruqi A, Shih JWK, Shih C. The mechanism of natural occurrence of two closely linked HBV precore predominant mutations. Virology 1995; 211: 144-56.
77. Loeb DD, Tian R. Transfer of the minus strand of DNA during hepadnavirus replication is not invariable but prefers a specific location. J. Virol. 1995; 69: 6886-91.
78. Nassal M, Rieger A. A bulged region of the hepatitis B virus RNA encapsidation signal contains the replication origin for discontinuous first-strand DNA synthesis. J. Virol. 1996; 70: 2764-73.
79. Wang GH, Seeger C. Novel mechanism for reverse transcription in hepatitis B virus J. Virol. 1993; 67: 6506-12.
80. Wang GH, Zoulim F, Leber EH, Kitson J, Seeger C. Role of RNA in enzymatic activity of the reverse transcriptase of hepatitis B viruses. J. Virol. 1994; 68: 8437-42.
81. Junker-Niepmann M, Bartenschlager R, Schaller H. A short cis-acting sequence is required for hepatitis B virus pregenome encapsidation and sufficient for packaging of foreign RNA. EMBO J. 1990; 9: 3389-96.
82. Fallows DA, Goff SP. Mutations in the epsilon sequences of human hepatitis B virus affect both RNA encapsidation and reverse transcription. J. Virol. 1995; 69: 3067-73.
83. Knaus T, Nassal M. The encapsidation signal on the hepatitis B virus RNA pregenome forms a stem-loop structure that is critical for its function. Nucleic Acids Res. 1993; 21: 3967-75.
84. Tavis JE, Perri S, Ganem D. Hepadnavirus reverse transcription initiates within the stem-loop of the RNA packaging signal and employs a novel strand transfer. J. Virol. 1994; 68: 3536-43.
85. Lok ASF, Akarca U, Greene S. Mutations in the precore region of hepatitis B virus serve to enhance the stability of the secondary structure of the pregenome encapsidation signal. Proc. Natl Acad. Sci. USA 1994; 91: 4077-81.
86. Lamberts C, Nassal M, Velhagen I, Zentgraf H, Schroder CH. Precore-mediated inhibition of hepatitis B virus progeny DNA synthesis. J. Virol. 1993; 67: 3756-62.
87. Guidotti LG, Matzke B, Pasquinelli C, Shoenberger JM, Rogler CE, Chisari FV. The hepatitis B virus (HBV) precore protein inhibits HBV replication in transgenic mice. J. Virol. 1996; 70: 7056-61.
88. Scaglioni PP, Melegari MM, Wands JR. Posttranscriptional regulation of HBV replication by the precore protein. In: Molecular Biology of Hepatitis B Viruses. Cold Spring Harbor: Cold Spring Harbor Laboratory, 1996; 92.
89. Kaneko M, Uchida T, Moriyama M et al. Probable implication of mutations of the X open reading frame in the onset of fulminant hepatitis B. J. Med. Virol. 1995; 47: 204-8.
90. Kurosaki M, Enomoto N, Asahina Y et al. Mutations in the core promoter region of the hepatitis B virus in patients with chronic hepatitis B. J. Med. Virol. 1996; 449: 115-23.
91. Laskus T, Rakela J, Nowichi MJ, Persing DH. Hepatitis B virus core promoter sequence analysis in fulminant and chronic hepatitis B. Gastroenterology 1995; 109: 1618-23.
92. McMillan JS, Bowden DS, Angus PW, McCaughan GW, Locarnini SA. Mutations in the hepatitis B virus precore/core gene and core promoter in patients with severe recurrent disease following liver transplantation. Hepatology 1996; 24: 1371-8.
93. Okamoto H, Tsuda F, Akahane Y et al Hepatitis B virus with mutations in the core promoter for an e antigen-negative phenotype in carriers with antibody to e antigen. J. Virol. 1994; 68: 8102-10.
94. Sato S, Suzuki K, Akahane Y et al. Hepatitis B virus strains with mutations in the core promoter in patients with fulminant hepatitis. Ann. Intern. Med. 1995; 122: 241-8.
95. 1764) of hepatitis B virus: Clinical significance and an easy method for detection. J. Gen. Virol. 1995; 76: 3159-64.
96. Lenhoff RJ, Summers J. Construction of avian hepadnavirus variants with enhanced replication and cytopathicity in primary hepatocytes. J. Virol. 1994; 68: 5706-13.
97. Blum HE, Zhang ZS, Galun E et al. Hepatitis B virus X protein is not central to the viral life cycle in vitro. J. Virol. 1992; 12: 4229-42.
98. Koike K, Shirakata Y, Yaginuma K et al. Oncogenic potential of hepatitis B virus. Mol. Biol. Med. 1989; 6: 151-60.
99. Chang C, Enders G, Sprengel R, Peters N, Varmus HE, Ganem D. Expression of the precore region of an avian hepatitis B virus is not required for viral replication. J. Virol. 1987; 61: 3322-5.
100. Chen HS, Kew MC, Hornbuckle WE et al. The precore gene of woodchuck hepatitis virus genome is not essential for viral replication in the natural host. J. Virol. 1992; 66: 5682-4.
101. Carlier D, Jean-Jean O, Rossignol JM. Characterization and biosynthesis of the woodchuck hepatitis virus e antigen. J. Gen. Virol. 1994; 75: 171-5.
102. Okada K, Kamiyama I, Inomata M, Imai M, Miyakawa Y, Mayumi M. e-Antigen and anti-e in the serum of asymptomatic carrier mothers as indicators of positive and negative transmission of hepatitis B virus to their infants. N. Engl. J. Med. 1976; 294: 746-9.
103. Milich DR, Jones JE, Hughes JL, Price J, Raney AK, McLachlan A. Is a function of the secreted hepatitis Be antigen to induce immunologie tolerance in utero? Proc. Natl Acad. Sci. USA 1990; 87: 6599-603.
104. Miller RH, Girones R, Cote PJ et al. Evidence against a requisite role for defective virus in the establishment of persistent hepadnavirus infection. Proc. Natl Acad. Sci. USA 1989; 87: 9329-32.
105. Schlesinger S, Weiss BG. Defective RNAs of Alpha-viruses. In: Schlesinger S, Schlesinger MJ, eds. The Togaviridae and Flaviviridae. New York: Plenum Press, 1986; 149-66.
106. Valenzuela P, Quiroga M, Zaldivar J, Gray P, Rutter WJ. The nucleotide sequence of the hepatitis B viral genome and the identification of the major genes. In: Fields BN, Haenisch R, Fox CF, eds. Animal Virus Genetics. New York: Academic Press, 1980; 57-70.
107. Girones R, Cote PJ, Hornbuckle WE et al. Complete nucleotide sequence of a molecular clone of woodchuck hepatitis virus that is infectious in the natural host. Proc. Natl Acad. Sci. USA 1989; 86: 1846-9.
108. Seeger C, Ganem D, Varmus HE. Nucleotide sequence of an infectious molecularly cloned genome of ground squirrel hepatitis virus. J. Virol. 1984; 51: 367-75.
109. Buckwold VE. Regulation of the core promoter of hepatitis B virus. Ph.D. thesis. Los Angeles: University of Southern California, 1996.