Design of high-activity mutants of human butyrylcholinesterase against (-)-cocaine: Structural and energetic factors affecting the catalytic efficiency

Biochemistry

Volumn 49, Issue 42, 2010, Pages 9113-9119

  Zheng, Fang ^a   Yang, Wenchao ^a   Xue, Liu ^a   Hou, Shurong ^a   Liu, Junjun ^a   Zhan, Chang Guo ^a  

^a University of Kentucky   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARBONYLATION; ENZYMES; ESTERIFICATION; ESTERS; HYDROGEN; HYDROGEN BONDS; MOLECULAR OXYGEN; POSITIVE IONS;

BUTYRYLCHOLINESTERASE; CARBONYL OXYGEN; CATALYTIC ACTIVITY; CATALYTIC EFFICIENCIES; CHEMICAL REACTION PROCESS; ENERGETIC FACTORS; EXPERIMENTAL DATA; EXPERIMENTAL TEST; HYDROGEN BONDINGS; OXYANION HOLE; PROTEIN STRUCTURES; TRANSITION STATE; WILD TYPES;

CATALYST ACTIVITY;

BENZOIC ACID; CHOLINESTERASE; COCAINE; MUTANT PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; CATALYSIS; DRUG DESIGN; HYDROGEN BOND; HYDROLYSIS; MUTANT; PRIORITY JOURNAL; AMINO ACID SUBSTITUTION; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME ACTIVE SITE; GENETICS; HUMAN; IN VITRO STUDY; KINETICS; METABOLISM; MOLECULAR DYNAMICS; PROTEIN ENGINEERING; SITE DIRECTED MUTAGENESIS;

AMINO ACID SUBSTITUTION; BUTYRYLCHOLINESTERASE; CATALYTIC DOMAIN; COCAINE; HUMANS; HYDROGEN BONDING; IN VITRO TECHNIQUES; KINETICS; MODELS, MOLECULAR; MOLECULAR DYNAMICS SIMULATION; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

EID: 77958086564     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi1011628     Document Type: Article

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