Selective covalent labeling of tag-fused GPCR proteins on live cell surface with a synthetic probe for their functional analysis

Journal of the American Chemical Society

Volumn 132, Issue 27, 2010, Pages 9301-9309

  Nonaka, Hiroshi ^a   Fujishima, Sho Hei ^a   Uchinomiya, Sho Hei ^a   Ojida, Akio ^a,b   Hamachi, Itaru ^a,b  

^a KYOTO UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLCHOLINE RECEPTOR; BIO-IMAGING; BIOTIN UNITS; BIOTIN-STREPTAVIDIN; BLOTTING ANALYSIS; CELL SURFACES; COVALENT LABELING; ENDOSOMES; FUNCTION ANALYSIS; FUNCTIONAL MOLECULES; G-PROTEIN COUPLED RECEPTORS; HEK293 CELLS; LABELING METHODS; LIVE CELL; LIVING CELL; MOLECULAR PROBES; PH CHANGE; PHYSIOLOGICAL CONDITION; PROTEIN FUNCTIONS; PROTEIN LABELING; RAPID REACTIONS; STIMULI-RESPONSIVE; SYNTHETIC PROBES; VERSATILE METHODS;

CELL MEMBRANES; COENZYMES; FLUOROPHORES; PROTEINS; VISUALIZATION; ZINC; ZINC COMPOUNDS;

PROBES;

BIOTIN; BRADYKININ RECEPTOR; G PROTEIN COUPLED RECEPTOR; MUSCARINIC RECEPTOR; STREPTAVIDIN; ZINC; CYSTEINE; FLUORESCEIN DERIVATIVE; MOLECULAR PROBE; OLIGOPEPTIDE; ORGANOMETALLIC COMPOUND; ZN(II)-DPATYR;

ARTICLE; BINDING AFFINITY; CELL STRAIN HEK293; CELL SURFACE; ENDOSOME; FLUORESCENCE; MOLECULAR PROBE; PH; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION; CELL LINE; CHEMISTRY; ENDOCYTOSIS; GENETIC PROCEDURES; HUMAN; METABOLISM; STAINING;

BIOTIN; CELL LINE; CYSTEINE; ENDOCYTOSIS; ENDOSOMES; FLUORESCEINS; HUMANS; MOLECULAR PROBE TECHNIQUES; MOLECULAR PROBES; OLIGOPEPTIDES; ORGANOMETALLIC COMPOUNDS; RECEPTORS, G-PROTEIN-COUPLED; STAINING AND LABELING;

EID: 77955840000     PISSN: 00027863     EISSN: 15205126     Source Type: Journal    
DOI: 10.1021/ja910703v     Document Type: Article

References (43)

1. (a) Schlessinger, J. Cell 2000, 103, 211-225.
2. (b) Verkman, A. S.; Mitra, A. K. Am. J. Physiol.: Renal. Physiol. 2000, 278, 13.
3. (c) Torres, G. E.; Gainetdinov, R. R.; Caron, M. G. Nat. Rev. Neurosci. 2003, 4, 13.
4. (a) O'Hare, H. M.; Johnsson, K.; Gautier, A. Curr. Opin. Biol. 2007, 17, 488.
5. (b) Chen, I.; Ting, A. Y. Curr. Opin. Biotechnol. 2005, 16, 1.
6. (c) Soh, N. Sensors 2008, 8, 1004.
7. (d) Fernández-Suárez, M.; Ting, A. Y. Nat. Rev. Mol. Cell Biol. 2008, 9, 929.
8. (e) Giepmans, B. N. G.; Adams, S. R.; Ellisman, M. H.; Tsien, R. Y. Science 2006, 312, 217.
9. (f) Terpe, K. Appl. Microbiol. Biotechno. 2003, 60, 523.
10. (a) Gronemeyer, T.; Godin, G.; Johnsson, K. Curr. Opin. Biotechnol. 2005, 16, 453.
11. (b) Carrico, I. S.; Carlson, B. L.; Bertozzi, C. R. Nat Chem. Biol. 2007, 3, 321.
12. (c) Los, G. V.; Wood, K. Methods Mol. Biol. 2006, 356, 195.
13. (d) Lin, C.-W.; Ting, A. Y. J. Am. Chem. Soc. 2006, 128, 4542.
14. (e) Chen, I.; Howarth, M.; Lin, W.; Ting, A. Y. Nat. Methods 2005, 2, 99.
15. (f) George, N.; Pick, H.; Vogel, H.; Johnsson, N.; Johnsson, K. J. Am. Chem. Soc. 2004, 126, 8896.
16. (g) Los, G. V.; et al. ACS Chem. Biol. 2008, 3, 373.
17. Keppler, A.; Gendreizig, S.; Gronemeyer, T.; Pick, H.; Vogel, H.; Johnsson, K. Nat. Biotechnol. 2003, 21, 86.
18. (a) Griffin, B. A.; Adams, S. R.; Tsien, R. Y. Science 1998, 281, 269.
19. (a) Halo, T. L.; Appelbaum, J.; Hobert, E. M.; Balkin, D. M.; Schepartz, A. J. Am. Chem. Soc. 2009, 131, 438.
20. (b) Hauser, C. T.; Tsien, R. Y. Proc. Natl. Acad. Sci. U. S. A. 2007, 104, 3693.
21. (c) Lata, S.; Gavutis, M.; Tampé, R.; Piehler, J. J. Am. Chem. Soc. 2006, 128, 2365.
22. (d) Guignet, E. G.; Hovius, R.; Vogel, H. Nat. Biotechnol. 2004, 22, 440.
23. (e) Yano, Y.; Yano, A.; Oishi, S.; Sugimoto, Y.; Tsujimoto, G.; Fujii, N.; Matsuzaki, K. ACS Chem. Biol. 2007, 3, 321.
24. (a) Ojida, A.; Honda, K.; Shinmi, D.; Kiyonaka, S.; Mori, Y.; Hamachi, I. J. Am. Chem. Soc. 2006, 128, 10452.
25. (b) Honda, K.; Nakata, E.; Ojida, A.; Hamachi, I. Chetm. Commun. 2006, 4024.
26. (c) Honda, K.; Fujishima, S.; Ojida, A.; Hamachi, I. ChemBioChem 2007, 8, 1370.
27. (d) Ojida, A.; Fujishima, S.; Honda, K.; Nonaka, H.; Uchinomiya, S.; Hamachi, I. Chem. Asian J. 2010, 5, 877.
28. Nonaka, H.; Tsukiji, S.; Ojida, A.; Hamachi, I. J. Am. Chem. Soc. 2007, 129, 15777.
29. (a) Mammen, M.; Choi, S.-K.; Whitesides, G. M. Angew. Chem., Int. Ed. 1998, 37, 2754.
30. (b) Gestwicki, J. E.; Cairo, C. W.; Strong, L. E.; Oetjen, K. A.; Kiessling, L. L. J. Am. Chem. Soc. 2002, 124, 14922.
31. (a) Leeb-Lundberg, L. M. F.; Marceau, F.; Muller-Esterl, W.; Pettibone, D. J.; Zuraw, B. L. Pharmacol. Rev. 2005, 57, 27.
32. (b) Marceau, F.; Regoli, D. Nat. Rev. Drug Discovery 2004, 3, 845.
33. Van Koppen, C. J.; Kaiser, B. Pharmacol. Ther. 2003, 98, 197.
34. (a) Michineaul, S.; Muller, L.; Pizard, A.; Alhenc-Gelas, F.; Rajerison, R. M. Biol. Chem. 2004, 385, 49.
35. (b) Blaukat, A.; Pizard, A.; Rajerison, R. M.; Alhenc-Gelas, F.; Muller-Esterl, W.; Dikic, I. FEBS Lett. 1999, 451, 337.
36. Pierce, K. L.; Premont, R. T.; Lefkowitz, R. J. Nat. Rev. Mol. Cell Biol. 2002, 3, 63.
37. (a) Howl, J.; Langel, U.; Hawtin, S. R.; Valkna, A.; Yarwood, N. J.; Saar, K.; Wheatley, M. FASEB J. 1997, 11, 582-590.
38. (b) Bachvarov, D. R.; Houle, S.; Bachvarova, M.; Bouthillier, J.; Adam, A.; Marceau, F. J. Pharmacol. Exp. Ther. 2001, 297, 19-26.
39. Shockley, M. S.; Burford, N. T.; Sadee, W.; Lameh, J. J. Neurochem. 1997, 68, 601-609.
40. (a) Urano, Y.; Asanuma, D.; Hama, Y.; Koyama, Y.; Barrett, T.; Kamiya, M.; Nagano, T.; Watanabe, T.; Hasegawa, A.; Choyke, P. L.; Kobayashi, H. Nat. Med. 2009, 15, 104-109.
41. (b) Maxfield, F. R.; McGraw, T. E. Nat. Rev. Mol. Cell Biol. 2004, 5, 121.
42. (c) Hanyaloglul, A. C.; Zastrowe, M. V. Annu. Rev. Pharmacol. Toxicol. 2008, 48, 537.
43. Adie, E. J.; Kalinka, S.; Smith, L.; Francis, M. J.; Marenghi, A.; Cooper, M. E.; Briggs, M.; Michael, N. P.; Milligan, G.; Game, S. BioTechniques 2002, 33, 1152.