Inulin and probiotics in newly weaned piglets: Effects on intestinal morphology, mRNA expression levels of inflammatory marker genes and haematology

Archives of Animal Nutrition

Volumn 64, Issue 4, 2010, Pages 304-321

  Mair, Christiane ^a   Plitzner, Christian ^a   Pfaffl, Michael W ^b   Schedle, Karl ^a   Meyer, Heinrich H D ^b   Windisch, Wilhelm ^a  

^a UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Gene expression; Haematology; Intestine; Inulin; Morphology; Piglets; Probiotics

Indexed keywords

INULIN; MESSENGER RNA; PROBIOTIC AGENT; GENETIC MARKER;

ANIMAL; ANIMAL DISEASE; ANIMAL FOOD; ARTICLE; BLOOD; CLINICAL TRIAL; COLON; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DIET; DIET SUPPLEMENTATION; DRUG EFFECT; FEMALE; GENE EXPRESSION REGULATION; GENETIC MARKER; GENETICS; HISTOLOGY; INFLAMMATION; INTESTINE; LYMPHOID TISSUE; MALE; METABOLISM; PHYSIOLOGY; SWINE; WEANING; ANALYSIS; ANATOMY AND HISTOLOGY; DRUG EFFECTS; VETERINARY;

ANIMAL FEED; ANIMAL NUTRITIONAL PHYSIOLOGICAL PHENOMENA; ANIMALS; COLON; DIET; DIETARY SUPPLEMENTS; FEMALE; GENE EXPRESSION REGULATION; GENETIC MARKERS; INFLAMMATION; INTESTINES; INULIN; LYMPHOID TISSUE; MALE; PROBIOTICS; RNA, MESSENGER; SWINE; WEANING;

ENTEROCOCCUS; LACTOBACILLUS;

EID: 77954274259     PISSN: 1745039X     EISSN: 14772817     Source Type: Journal    
DOI: 10.1080/1745039X.2010.492137     Document Type: Article

References (58)

1. Apgar GA, Kornegay ET, Lindemann MD, Wood CM. 1993. The effect of feeding various levels of Bifidobacterium globosum A on the performance, gastrointestinal measurements, and immunity of weanling pigs and on the performance and carcass measurements of growing-finishing pigs. J Anim Sci. 71:2173-2179.
2. Baum B, Liebler-Tenorio EM, Enss ML, Pohlenz JF, Breves G. 2002. Saccharomyces boulardii and Bacillus cereus var. toyoi influence the morphology and the mucins of the intestine of pigs. Z Gastroenterol. 40:277-284.
3. Boatright KM, Salvesen GS. 2003. Mechanisms of caspase activation. Curr Opin Cell Biol. 15:725-731.
4. Bontempo V, Di Giancamillo A, Savoini G, Dell'Orto V, Domeneghini C. 2006. Live yeast dietary supplementation acts upon intestinal morpho-functional aspects and growth in weanling piglets. Anim Feed Sci Technol. 129(3-4):224-236.
5. Canibe N, Miettinen H, Jensen BB. 2008. Effect of adding Lactobacillus plantarum or a formic acid containing-product to fermented liquid feed on gastrointestinal ecology and growth performance of piglets. Livest Sci. 114:251-262.
6. Cetin N, Guclu BK, Cetin E. 2005. The effects of probiotic and mannanoligosaccharide on some haematological and immunological parameters in turkeys. J Vet Med A Physiol Pathol Clin Med. 52:263-267.
7. Chichlowski M, Croom WJ, Edens FW, McBride BW, Qiu R, Chiang CC, Daniel LR, Havenstein GB, Koci MD. 2007. Microarchitecture and spatial relationship between bacteria and ileal, cecal, and colonic epithelium in chicks fed a direct-fed microbial, PrimaLac, and salinomycin. Poult Sci. 86:1121-1132.
8. Cummings JH, Macfarlane GT. 1991. A review: The control and consequences of bacterial fermentation in the human colon. J Appl Microbiol. 70:443-459.
9. Deplancke B, Gaskins HR. 2001. Microbial modulation of innate defense: Goblet cells and the intestinal mucus layer. Am J Clin Nutr. 73:1131S-1141S.
10. Di Giancamillo A, Vitari F, Savoini G, Bontempo V, Bersani C, Dell'Orto V, Domeneghini C. 2008. Effects of orally administered probiotic Pediococcus acidilactici on the small and large intestine of weaning piglets. A qualitative and quantitative micro-anatomical study. Histol Histopathol. 23:651-664.
11. Dohrman A, Miyata S, Gallup M, Li JD, Chapelin C, Coste A, Escudier E, Nadel J, Basbaum C. 1998. Mucin gene (MUC 2 and MUC 5AC) upregulation by Gram-positive and Gramnegative bacteria. Biochim Biophys Acta. 1406:251-259.
12. Domeneghini C, Di Giancamillo A, Arrighi S, Bosi G. 2006. Gut-trophic feed additives and their effects upon the gut structure and intestinal metabolism. State of the art in the pig, and perspectives towards humans. Histol Histopathol. 21:273-283.
13. Eberhard M, Hennig U, Kuhla S, Brunner RM, Kleessen B, Metges CC. 2007. Effect of inulin supplementation on selected gastric, duodenal, and caecal microbiota and short chain fatty acid pattern in growing piglets. Arch Anim Nutr. 61:235-246.
14. Erickson KL, Hubbard NE. 2000. Probiotic immunomodulation in health and disease. J Nutr. 130(2S Suppl):403S-409S.
15. Fan YK, Croom J, Christensen VL, Black BL, Bird AR, Daniel LR, McBride BW, Eisen EJ. 1997. Jejunal glucose uptake and oxygen consumption in turkey poults selected for rapid growth. Poult Sci. 76:1738-1745.
16. Fooks LJ, Gibson GR. 2002. Probiotics as modulators of the gut flora. Brit J Nutr. 88(Suppl 1):S39-49.
17. Fuller R. 1989. Probiotics in man and animals. J Appl Bacteriol. 66:365-378.
18. Gibson GR, Roberfroid MB. 1995. Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J Nutr. 125:1401-1412.
19. Gibson GR, Probert HM, Loo JV, Rastall RA, Roberfroid MB. 2004. Dietarymodulation of the human colonic microbiota: Updating the concept of prebiotics. Nutr Res Rev. 17:259-275.
20. Gladden AB, Diehl JA. 2005. Location, location, location: The role of cyclin D1 nuclear localization in cancer. J Cell Biochem. 96:906-913.
21. Huang CH, Qiao SY, Li DF, Piao XS, Ren JP. 2004. Effects of Lactobacilli on the performance, diarrhea incidence, VFA concentration and gastrointestinal microbial flora of weaning pigs. Asian Austral J Anim. 17:401-409.
22. Jensen BB. 1998. The impact of feed additives on the microbial ecology of the gut in young pigs. J Anim Feed Sci. 7:45-64.
23. Kandori H, Hirayama K, Takeda M, Doi K. 1996. Histochemical, lectin-histochemical and morphometrical characteristics of intestinal goblet cells of germfree and conventional mice. Exp Anim. 45:155-160.
24. Kaufhold J, Hammon HM, Blum JW. 2000. Fructo-oligosaccharide supplementation: effects on metabolic, endocrine and hematological traits in veal calves. J Vet Med A Physiol Pathol Clin Med. 47:17-29.
25. Klose V, Bruckbeck R, Henikl S, Schatzmayr G, Loibner AP. 2010. Identification and antimicrobial susceptibility of porcine bacteria that inhibit the growth of Brachyspira hyodysenteriae in vitro. J Appl Microbiol. 108:1271-1280.
26. Lodemann U, Lorenz BM, Weyrauch KD, Martens H. 2008. Effects of Bacillus cereus var. toyoi as probiotic feed supplement on intestinal transport and barrier function in piglets. Arch Anim Nutr. 62:87-106.
27. Mair C, Plitzner C, Domig KJ, Schedle K, Windisch W. 2010. Impact of inulin and a multispecies probiotic formulation on performance, microbial ecology and concomitant fermentation patterns in newly weaned piglets. J Anim Physiol Anim Nutr. (Berl) [Internet]. [Published online 2010 Jun 22]; DOI: 10.1111/j.1439-0396.2010.01000.x.
28. Marinho MC, Pinho MA, Mascarenhas RD, Silva FC, Lordelo MM, Cunha LF, Freire JPB. 2007. Effect of prebiotic or probiotic supplementation and ileo rectal anastomosis on intestinal morphology of weaned piglets. Livest Sci. 108:240-243.
29. Matsuo K, Ota H, Akamatsu T, Sugiyama A, Katsuyama T. 1997. Histochemistry of the surface mucous gel layer of the human colon. Gut. 40:782-789.
30. McCracken BA, Spurlock ME, Roos MA, Zuckermann FA, Gaskins HR. 1999. Weaning anorexia may contribute to local inflammation in the piglet small intestine. J Nutr. 129:613-619.
31. Murtaugh MP. 1994. Porcine cytokines. Vet Immunol Immunopathol. 43:37-44.
32. Neish AS. 2009. Microbes in gastrointestinal health and disease. Gastroenterology. 136:65-80.
33. Perdigon G, Maldonado Galdeano C, Valdez JC, Medici M. 2002. Interaction of lactic acid bacteria with the gut immune system. Eur J Clin Nutr. 56(Suppl. 4):S21-26.
34. Pfaffl MW. 2001. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29:e45.
35. Pluske JR, Hampson DJ, Williams IH. 1997. Factors influencing the structure and function of the small intestine in the weaned pig: A review. Livest Prod Sci. 51:215-236.
36. Rao AV. 1999. Dose-response effects of inulin and oligofructose on intestinal bifidogenesis effects. J Nutr. 129(7 Suppl):1442S-1445S.
37. Richards JD, Gong J, de Lange CFM. 2005. The gastrointestinal microbiota and its role in monogastric nutrition and health with an emphasis on pigs: Current understanding, possible modulations, and new technologies for ecological studies. Can J Anim Sci. 85:421-435.
38. Roberfroid MB. 1998. Prebiotics and synbiotics: Concepts and nutritional properties. Brit J Nutr. 80:S197-202.
39. Roller M, Rechkemmer G, Watzl B. 2004. Prebiotic inulin enriched with oligofructose in combination with the probiotics Lactobacillus rhamnosus and Bifidobacterium lactis modulates intestinal immune functions in rats. J Nutr. 134:153-156.
40. Rossi F, Morlacchini M, Gatti P, Soldi S, Callegari ML, Piva G. 2008. Effects of a gluco-oligosaccaride supplement on the morphological characteristics of the gastro-intestinal tract and growth performance in weaned piglets. Ital J Anim Sci. 7:185-198.
41. Rycroft CE, Jones MR, Gibson GR, Rastall RA. 2001. A comparative in vitro evaluation of the fermentation properties of prebiotic oligosaccharides. J Appl Microbiol. 91:878-887.
42. Salyers AA, West SE, Vercellotti JR, Wilkins TD. 1977. Fermentation of mucins and plant polysaccharides by anaerobic bacteria from the human colon. Appl Environ Microbiol. 34:529-533.
43. Scharek L, Guth J, Filter M, Schmidt MF. 2007. Impact of the probiotic bacteria Enterococcus faecium NCIMB 10415 (SF68) and Bacillus cereus var. toyoi NCIMB 40112 on the development of serum IgG and faecal IgA of sows and their piglets. Arch Anim Nutr. 61:223-234.
44. Schedle K, Pfaffl MW, Plitzner C, Meyer HHD, Windisch W. 2008. Effect of insoluble fibre on intestinal morphology and mRNA expression pattern of inflammatory, cell cycle and growth marker genes in a piglet model. Arch Anim Nutr. 62:427-438.
45. Schrezenmeir J, de Vrese M. 2001. Probiotics, prebiotics, and synbiotics - approaching a definition. Am J Clin Nutr. 73(2 Suppl):361S-364S.
46. Schroeder A, Mueller O, Stocker S, Salowsky R, Leiber M, Gassmann M, Lightfoot S, Menzel W, Granzow M, Ragg T. 2006. The RIN: An RNA integrity number for assigning integrity values to RNA measurements. BMC Mol Biol. 7:1-14.
47. Shim SB, Verstegen MW, Kim IH, Kwon OS, Verdonk JM. 2005. Effects of feeding antibiotic-free creep feed supplemented with oligofructose, probiotics or synbiotics to suckling piglets increases the preweaning weight gain and composition of intestinal microbiota. Arch Anim Nutr. 59:419-427.
48. Smirnov A, Perez R, Amit-Romach E, Sklan D, Uni Z. 2005. Mucin dynamics and microbial populations in chicken small intestine are changed by dietary probiotic and antibiotic growth promoter supplementation. J Nutr. 135:187-192.
49. Stewart ML, Timm DA, Slavin JL. 2008. Fructooligosaccharides exhibit more rapid fermentation than long-chain inulin in an in vitro fermentation system. Nutr Res. 28:329-334.
50. Tejada-Simon MV, Pestka JJ. 1999. Proinflammatory cytokine and nitric oxide induction in murine macrophages by cell wall cytoplasmic extracts of lactic acid bacteria. J Food Protect. 62:1435-1444.
51. Touchette KJ, Carroll JA, Allee GL, Matteri RL, Dyer CJ, Beausang LA, Zannelli ME. 2002. Effect of spray-dried plasma and lipopolysaccharide exposure on weaned pigs: I. Effects on the immune axis of weaned pigs. J Anim Sci. 80:494-501.
52. Walsh MC, Gardiner GE, Hart OM, Lawlor PG, Daly M, Lynch B, Richert BT, Radcliffe S, Giblin L, Hill C, Fitzgerald GF, Stanton C, Ross P. 2008. Predominance of a bacteriocin-producing Lactobacillus salivarius component of a five-strain probiotic in the porcine ileum and effects on host immune phenotype. FEMS Microbiol Ecol. 64:317-327.
53. Watzl B, Girrbach S, Roller M. 2005. Inulin, oligofructose and immunomodulation. Brit J Nutr. 93:S49-55.
54. Williams BA, Verstegen MWA, Tamminga S. 2001. Fermentation in the large intestine of single-stomached animals and its relationship to animal health. Nutr Res Rev. 14:207-227.
55. Windisch W, Schedle K, Plitzner C, Kroismayr A. 2008. Use of phytogenic products as feed additives for swine and poultry. J Anim Sci. 86(14 Suppl):E140-148.
56. Winkler P, Ghadimi D, Schrezenmeir J, Kraehenbuhl JP. 2007. Molecular and cellular basis of microflora-host interactions. J Nutr. 137(3 Suppl. 2):756S-772S.
57. Xu ZR, Zou XT, Hu CH, Xia MS, Zhan XA, Wang MQ. 2002. Effects of dietary fructooligosaccharide on digestive enzyme activities, intestinal microflora and morphology of growing pigs. Asian-Australas J Anim Sci. 15:1784-1789.
58. Zhou JS, Shu Q, Rutherfurd KJ, Prasad J, Birtles MJ, Gopal PK, Gill HS. 2000. Safety assessment of potential probiotic lactic acid bacterial strains Lactobacillus rhamnosus HN001, Lb. acidophilus HN017, and Bifidobacterium lactis HN019 in BALB/c mice. Int J Food Microbiol. 56:87-96.