Characterization of conformational changes and noncovalent complexes ofmyoglobin by electrospray ionizationmass spectrometry, circular dichroism and fluorescence spectroscopy

Journal of Mass Spectrometry

Volumn 45, Issue 6, 2010, Pages 618-626

  Lin, Xin ^a,b   Zhao, Wenjie ^a   Wang, Xian ^a  

^a SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES   (China)

^b HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Circular dichroism spectroscopy; CO Mb complex; Conformational changes; Electrospray ionization mass spectrometry; Fluorescence spectroscopy; H2O Mb complex; Heme proteins; Myoglobin

Indexed keywords

APOMYOGLOBIN; CIRCULAR DICHROISM; CONFORMATIONAL CHANGE; CONFORMATIONAL CHANGES; DIFFERENT SOLVENTS; ELECTROSPRAY IONIZATION MASS SPECTROMETRY; HEME GROUP; HEME IRON; HEME PROTEINS; LIGAND BINDING; METMYOGLOBIN; NATIVE STATE; NON-COVALENT COMPLEXES; ORGANIC CONTENT; OXIDATION STATE; PROTEIN INTERACTION; SOLUTION CONDITIONS; SPECTROSCOPIC DATA; SPECTROSCOPIC TECHNIQUE; TERTIARY STRUCTURES;

ACETONITRILE; COMPLEXATION; DICHROISM; ELECTROSPRAY IONIZATION; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; HEMOGLOBIN; IONIZATION; IRON; LIGANDS; MASS SPECTROMETRY; METHANOL; ORGANIC SOLVENTS; PARTICLE DETECTORS; PORPHYRINS;

CIRCULAR DICHROISM SPECTROSCOPY;

ACETONITRILE; ACID; APOMYOGLOBIN; HEME; HEMOPROTEIN; IRON; LIGAND; METHANOL; METMYOGLOBIN; MYOGLOBIN; ORGANIC SOLVENT;

ARTICLE; CIRCULAR DICHROISM; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; FLUORESCENCE SPECTROSCOPY; LIGAND BINDING; OXIDATION; PH; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN INTERACTION; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; SOLVENT EFFECT;

ACETONITRILES; ANIMALS; BENZENESULFONATES; CARBON MONOXIDE; CIRCULAR DICHROISM; HEME; HORSES; HYDROGEN-ION CONCENTRATION; METHANOL; MYOGLOBIN; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN DENATURATION; SPECTROMETRY, FLUORESCENCE; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; WATER;

EID: 77953174411     PISSN: 10765174     EISSN: 10969888     Source Type: Journal    
DOI: 10.1002/jms.1747     Document Type: Article

References (53)

1. B. Domon, R. Aebersold. Mass spectrometry and protein analysis. Science 2006, 312(5771), 212.
2. J. B. Fenn, M. Mann, C. K. Meng, S. F. Wong, C. M. Whitehouse. Electrospray ionization for mass spectrometry of large biomolecules. Science 1989, 246(4926), 64.
3. C. H. Chen. Review of a current role of mass spectrometry for proteome research. Anal. Chim. Acta 2008, 624(1), 16.
4. M. Bantscheff, M. Schirle, G. Sweetman, J. Rick, B. Kuster. Quantitative mass spectrometry in proteomics: a critical review. Anal. Bioanal. Chem. 2007, 389(4), 1017.
5. A. Honda, Y. Suzuki, K. Suzuki. Review of molecular modification techniques for improved detection of biomolecules by mass spectrometry. Anal. Chim. Acta 2008, 623(1), 1.
6. M.Y.Choi, Y.J.An, S.H.Kim, S.H.Roh, H. K.Ju, S.S.Hong, J.H. Park,K. J. Cho,D. W. Choi,S.W. Kwon. Mass spectrometry based proteomic analysis of human stem cells: a brief review. Exp. Mol. Med. 2007, 39(6), 690.
7. J. A. Loo. Electrospray ionization mass spectrometry: a technology for studying noncovalent macromolecular complexes. Int. J. Mass Spectrom. 2000, 200(1-3), 175.
8. J. A. Loo. Studying noncovalent protein complexes by electrospray ionization mass spectrometry. Mass Spectrom. Rev. 1997, 16(1), 1.
9. T. D. Veenstra. Electrospray ionization mass spectrometry in the study of biomolecular non-covalent interactions. Biophys. Chem. 1999, 79(2), 63.
10. J. A. Loo, C. V. Robinson. Review of the 19th Asilomar Conference on Mass Spectrometry: bimolecular interactions: identification and characterization of protein complexes. J. Am. Soc. Mass Spectrom. 2004, 15(5), 759.
11. S. Zhang, C. K. V. Pelt, D. B. Wilson. Quantitative determination of noncovalent binding interactions using automated nanoelectrospray mass spectrometry. Anal. Chem. 2003, 75(13), 3010.
12. V. J. Nesatyy. Gas-phasebinding of non-covalent protein complexes between bovine pancreatic trypsin inhibitor and its target enzymes studied by electrospray ionization tandem mass spectrometry. J.Mass Spectrom. 2001, 36(8), 950.
13. B.N. Pramanik, P. L. Bartner, U. A. Mirza, Y.H. Liu, A. K. Ganguly. Electrospray ionization mass spectrometry for the study of noncovalent complexes: an emerging technology. J. Mass Spectrom. 1998, 33(10), 911.
14. J. Anichina, D. K. Bohme. Mass-spectrometric studies of the interactions of selected metalloantibiotics and drugs with deprotonated hexadeoxynucleotide GCATGC.J.Phys.Chem.B 2009, 113(1), 328.
15. S. W. Feil, G. K. Koyanagi, J. Anichina, D. K. Bohme.Chemical stability and reactivity of deprotonated oligonucleotides (DNA) in the gas phase: protonation and solvation with hydrogen bromide. J. Phys. Chem. B 2008, 112(33), 10375.
16. K. De Vriendt, K. Sandra, T. Desmet, W. Nerinckx, J. Van Beeumen, B. Devreese. Evaluation of automated nano-electrospray mass spectrometry in the determination of non-covalent protein-ligand complexes. Rapid Commun.Mass Spectrom. 2004, 18(24), 3061.
17. E. Bordini, M. Hamdan. Investigation of some covalent and noncovalent complexes by matrix-assisted laser desorption/ionization time-of-flight and electrospray mass spectrometry. Rapid Commun.Mass Spectrom. 1999, 13(12), 1143.
18. S. Wendt, G. McCombie, J. Daniel, A. Kienhöfer,D. Hilvert, R. Zenobi. Quantitative evaluation of noncovalent chorismate mutaseinhibitor binding by ESI-MS. J.Am. Soc.Mass Spectrom. 2003, 14(12), 1470.
19. B. N. Green, T. Hutton, S. N. Vinogradov,Analysis of complex protein and glycoprotein mixtures by electrospray ionization mass spectrometry with maximum entropy processing. In Methods in Molecular Biology (Protein and Peptide Analysis by Mass Spectrometry); J. R. Chapman (Ed.), Humana Press Inc.: Totowa, NJ, 1996, Vol. 61, pp 279-294.
20. A. P. de Brouwer, C. Versluis, J. Westerman, B. Roelofsen, A. J. Heck, K. W. Wirtz. Determination of the stability of the noncovalent phospholipid transfer protein-Lipid complex by electrospray time-of-flight mass spectrometry. Biochemistry 2002, 41(25), 8013.
21. J. W. H. Wong, S. D. Maleknia, K. M. Downard. Study of the ribonuclease-S-protein-peptide complex using a radical probe and electrospray ionization mass spectrometry. Anal.Chem. 2003, 75(7), 1557.
22. S. V. Evans, G. D. Brayer. High-resolution study of the threedimensional structure of horse heart metmyoglobin. J.Mol. Biol. 1990, 213(4), 885.
23. R. K. Upmacis, D. P. Hajjar, B. T. Chait, U. A. Mirza. Direct observation of nitrosylatedhemein myoglobin and hemoglobin by electrospray ionization mass spectrometry. J. Am. Chem. Soc. 1997, 119(43), 10424.
24. W. Cao, X. Ye, G. Y. Georgiev, S. Berezhna, T. Sjodin, A. A. Demidov, W. Wang, J. T. Sage, P. M. Champion. Proximal and Distal Influences on Ligand Binding Kinetics in Microperoxidase and Heme Model Compounds. Biochemistry 2004, 43, 7017.
25. M. Otto, F. Knabe, U. Claußnitzer. Response surface study on protein charge-state distributions in electrospray ionization mass spectrometry related tomultiple ion equilibria in solution. Chemom. Intell. Lab. Syst. 2004, 72, 245.
26. S. S. Ray, S. K. Singh, P. Balaram. An Electrospray Ionization Mass Spectrometry Investigation of 1-Anilino-8-Naphthalene-Sulfonate (ANS) Binding to Proteins. J.Am. Soc. Mass Spectrom. 2001, 12, 428.
27. S. S. Ray, P. Balaram. 1-Anilino-8-naphthalene-sulfonate (ANS) Binding to Proteins Investigated by Electrospray Ionization Mass Spectrometry: Correlation of Gas-Phase Dye Binding to Population of Molten Globule States in Solution. J. Phys. Chem. B 1999, 103, 7068.
28. K. R. Babu, D. J. Douglas. Methanol-induced conformations of myoglobin at pH 4.0. Biochemistry 2000, 39(47), 14702.
29. O. O. Sogbein, D. A. Simmons, L. Konermann. Effects of pH on the kinetic reaction mechanism of myoglobin unfolding studied by time-resolved electrospray ionization mass spectrometry. J. Am. Soc. Mass Spectrom. 2000, 11(4), 312.
30. Y.-T. Li, Y.-L. Hsieh, J. D. Henion, B. Ganem. Studies on heme binding in myoglobin, hemoglobin, and cytochrome c by ion spray mass spectrometry. J. Am. Soc.Mass Spectrom. 1993, 4(8), 631.
31. L. Konermann, F. I. Rosell, A. G. Mauk, D. J. Douglas. Acid-induced denaturation of myoglobin studied by time-resolved electrospray ionization mass spectrometry. Biochemistry 1997, 36(21), 6448.
32. A. Ostermann, R. Waschipky, F. G. Parak, G. U. Nienhaus. Ligand binding and conformational motions in myoglobin. Nature 2000, 404(6774), 205.
33. V. Šrajer, T. Teng, T. Ursby, C. Pradervand, Z. Ren, S. Adachi, W. Schildkamp, D. Bourgeois, M. Wulff, Photolysis of the Carbon Monoxide Complex of Myoglobin: Nanosecond Time-Resolved Crystallography. Science 6 1996, 274(5293), 1726.
34. A. Ansari, C. M. Jones, E. R. Henry, J. Hofrichter, W. A. Eaton. Conformational relaxation and ligand binding in myoglobin. Biochemistry 1994, 33(17), 5128.
35. Y. Gao, S. F. El-Mashtoly, B. Pal, T. Hayashi, K. Harada, T. Kitagawa. Pathway of information transmission from heme to protein upon ligandbinding/dissociation inmyoglobin revealedby UV resonance raman spectroscopy. J. Bio. Chem. 2006, 281(34), 24637.
36. 2 binding to horse heart myoglobin reconstituted with synthetic iron chlorin green hemes. Inorg. Chim.Acta 2000, 311(1-2), 113.
37. L. M. Blomberg, M. R. A. Blomberg, P. E. M. Siegbahn. A theoretical study on the binding of O2, NO and CO to heme proteins. J. Inorg. Biochem. 2005, 99(4), 949.
38. K. Nienhaus, G. U. Nienhaus. Influence of distal residue B10 on CO dynamics in myoglobin and neuroglobin J. Biol.Phys. 2007, 33(5-6), 357.
39. B. T. Chait, S. B. Kent. Weighing naked proteins: practical, high-accuracy mass measurement of peptides and proteins. Science 1992, 257(5078), 1885.
40. R. Grandori, I. Matecko, N. Müller. Uncoupled analysis of secondary and tertiary protein structure by circular dichroism and electrospray ionization mass spectrometry J.Mass Spectrom. 2002, 37(2), 191.
41. L. Konermann, D. J. Douglas. Equilibrium unfolding of proteins monitored by electrospray ionization mass spectrometry: Distinguishing two-state from multi-state transitions. Rapid Commun.Mass Spectrom. 1998, 12(8), 435.
42. U. A. Mirza, S. L. Cohen, B. T. Chait. Heat-induced conformational changes in proteins studied by electrospray ionization mass spectrometry. Anal. Chem. 1993, 65(1), 1.
43. V. Katta, B. T. Chait. Conformational changes in proteins probed by hydrogen-exchange electrospray-ionization mass spectrometry. Rapid Commun.Mass Spectrom. 1991, 5(4), 214.
44. J. A. Loo, R. R. Loo, H. R. Udseth, C. G. Edmonds, R. D. Smith. Solvent-induced conformational changes of polypeptides probed by electrospray-ionizationmass spectrometry. Rapid Commun.Mass Spectrom. 1991, 5(3), 101.
45. F. M. Hughson, P. E. Wright, R. L. Baldwin. Structural characterization of a partly folded apomyoglobin intermediate Science 1990, 249(4976), 1544.
46. K. Gekko, E. Ohmae, K. Kameyama, T. Takagi. Acetonitrile-protein interactions: amino acid solubility and preferential solvation Biochim. Biophys. Acta 1998, 1387(1-2), 195.
47. P. Kebarle, L. Tang. From ions in solution to ions in the gas phase - the mechanism of electrospray mass spectrometry. Anal. Chem. 1993, 65, 972A.
48. S. Zhou, B. S. Prebyl, K. D. Cook. Profiling pH changes in the electrospray plume. Anal. Chem. 2002, 74(19), 4885.
49. A. Wortmann, A. Kistler-Momotova, R. Zenobi, M. C. Heine, O. Wilhelm, S. E. Pratsinis. Shrinking Droplets in Electrospray Ionization and Their Influence on Chemical Equilibria. J. Am. Soc. Mass Spectrom. 2007, 18(3), 385.
50. M. C. Heine, S. E. Pratsinis. Droplet and particle dynamics during flame spray synthesis of nanoparticles Ind. Eng. Chem. Res. 2005, 44(16), 6222.
51. B. Abramzon, W. A. Sirignano. Droplet vaporization model for spray combustion calculations. Int. J. Heat Mass Transfer 1989, 32(9), 1605.
52. Z. Olumee, J. H. Callahan, A. Vertes. Velocity compression in cylindrical capacitor electrospray ofmethanol-watermixtures Anal. Chem. 1999, 71(18), 4111.
53. S. D. Longa, S. Pin, R. Cortès, A. V. Soldatov, B. Alpert. Fe-Heme conformations in ferric myoglobin. Biophys. J. 1998, 75(6), 3154.