Key Amino Acid Residues Involved in Multi-Point Binding Interactions between Brazzein, a Sweet Protein, and the T1R2-T1R3 Human Sweet Receptor

Journal of Molecular Biology

Volumn 398, Issue 4, 2010, Pages 584-599

  Assadi Porter, Fariba M ^a   Maillet, Emeline L ^b   Radek, James T ^a   Quijada, Jeniffer ^b   Markley, John L ^a   Max, Marianna ^b  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

Calcium assay; G protein coupled receptor; Heterodimeric sweet receptor; Psychophysical assay; Sweet protein

Indexed keywords

BRAZZEIN PROTEIN; CYCLAMATE SODIUM; G PROTEIN COUPLED RECEPTOR; MONELLIN; NEOTAME; SUCRALOSE; SWEET PROTEIN; TASTE TYPE 1 RECEPTOR 2; TASTE TYPE 1 RECEPTOR 3; UNCLASSIFIED DRUG; BRAZZEIN PROTEIN, PENTADIPLANDRA BRAZZEANA; MUTANT PROTEIN; TASTE RECEPTORS, TYPE 1; VEGETABLE PROTEIN;

ACIDITY; AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CHIMERA; CONTROLLED STUDY; DISULFIDE BOND; FEMALE; HUMAN; HUMAN CELL; HUMAN EXPERIMENT; HYDROGEN BOND; IN VITRO STUDY; LIGAND BINDING; MALE; MUTAGENESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; PROTEIN QUATERNARY STRUCTURE; RECEPTOR BINDING; RESIDUE ANALYSIS; TASTE BUD; BIOLOGICAL MODEL; CELL LINE; CHEMICAL STRUCTURE; GENETICS; METABOLISM; PROTEIN ANALYSIS; PROTEIN BINDING; SITE DIRECTED MUTAGENESIS;

DIONAEA;

AMINO ACID SUBSTITUTION; CELL LINE; HUMANS; MODELS, BIOLOGICAL; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; PLANT PROTEINS; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; PROTEIN STRUCTURE, QUATERNARY; RECEPTORS, G-PROTEIN-COUPLED;

EID: 77951901176     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2010.03.017     Document Type: Article

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