Monkey electrophysiological and human psychophysical responses to mutants of the sweet protein brazzein: Delineating brazzein sweetness

Chemical Senses

Volumn 28, Issue 6, 2003, Pages 491-498

  Jin, Zheyuan ^a   Danilova, Vicktoria ^a   Assadi Porter, Fariba M ^a   Markley, John L ^a   Hellekant, Göran ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Low caloric natural sweeteners; Magnitude labeled scale; Rhesus monkey; Sweet taste receptor

Indexed keywords

ALANINE; ASPARAGINE; ASPARTIC ACID; GLUTAMIC ACID; LYSINE; MONELLIN; MUTANT PROTEIN; PROTEIN BRAZZEIN; SWEET PROTEIN; UNCLASSIFIED DRUG; BRAZZEIN PROTEIN, PENTADIPLANDRA BRAZZEANA; SWEETENING AGENT; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ANALYTIC METHOD; ARTICLE; CARBOXY TERMINAL SEQUENCE; CHORDA TYMPANI; CONTROLLED STUDY; CORRELATION COEFFICIENT; EXPERIMENTAL DESIGN; FEMALE; HUMAN; HUMAN EXPERIMENT; HYPOTHESIS; MACACA; MALE; MUTATIONAL ANALYSIS; NERVE FIBER; NONHUMAN; NORMAL HUMAN; POINT MUTATION; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PSYCHOPHYSIOLOGY; RECORDING; RHESUS MONKEY; SCORING SYSTEM; SENSORY NERVE; SENSORY STIMULATION; SENSORY SYSTEM ELECTROPHYSIOLOGY; STATISTICAL SIGNIFICANCE; SWEETNESS; ANIMAL; COMPARATIVE STUDY; ELECTROPHYSIOLOGY; GENETICS; MUTATION; PHYSIOLOGY; PSYCHOPHYSICS; TASTE;

CHORDA; MACACA MULATTA; PRIMATES;

ANIMALS; CHORDA TYMPANI NERVE; ELECTROPHYSIOLOGY; FEMALE; HUMANS; MACACA MULATTA; MALE; MUTATION; NERVE FIBERS; PLANT PROTEINS; PSYCHOPHYSICS; SWEETENING AGENTS; TASTE;

EID: 3042511803     PISSN: 0379864X     EISSN: None     Source Type: Journal    
DOI: 10.1093/chemse/28.6.491     Document Type: Article

References (27)

1. Assadi-Porter, F.M., Aceti, D.J., Cheng, H. and Markley, J.L. (2000a) Efficient production of recombinant brazzein, a small, heat-stable, sweet-tasting protein of plant origin. Arch. Biochem. Biophys., 376, 252-258.
2. Assadi-Porter, F.M., Aceti, D.J. and Markley, J.L. (2000b) Sweetness determinant sites of brazzein, a small, heat-stable, sweet-tasting protein. Arch. Biochem. Biophys., 376, 259-265.
3. Assadi-Porter, F.M., Abildgaard, F., Blad, H. and Markley, J.L. (2003) Correlation of the sweetness of variants of the protein brazzein with patterns of hydrogen bonds detected by NMR spectroscopy. J. Biol. Chem., in press.
4. Caldwell, J.E., Abildgaard, F., Dzakula, Z., Ming, D., Hellekant, G. and Markley, J.L. (1998a) Solution structure of the thermostable sweet-tasting protein brazzein. Nat. Struct, Biol., 5, 427-431.
5. Caldwell, J.E., Abildgaard, F., Ming, D., Hellekant, G. and Markley, J.L. (1998b) Complete 1H and partial 13C resonance assignments at 37 and 22 degrees C for brazzein, an intensely sweet protein. J. Biomol. NMR, 11, 231-232.
6. Danilova, V. and Hellekant, G. (2003) Comparison of the responses of the chorda tympani and glossopharyngeal nerves to taste stimuli in C57BL/6J mice. BMC Neurosci., 4, 5.
7. Danilov, Y., Danilova, V. and Hellekant, G. (2002a) Specificity of sucrose-best fibers in rhesus monkey chorda tympani does not depend on stimulus concentration. AChems XXIV, 57:228.
8. Danilova, V., Danilov, Y., Roberts, T., Elmer, D. and Hellekant, G. (2002b) Electrophysiological recordings of mammalian taste nerves. In Simon, S.A. and Nicolelis, M.A.L. (eds), Methods and Frontiers in Neuroscience. CRC Press, Boca Raton, FL, pp. 239-264.
9. Danilova, V., Danilov, Y, Roberts, T., Tinti, J.-M., Nofre, C. and Hellekant, G. (2002c) The sense of taste in a New World monkey, the common marmoset: recordings from the chorda tympani and glossopharyngeal nerves. J. Neurophysiol., 88, 579-594.
10. DeRider, M.L. (2001) Conformational Investigations: I. Prolyl Ring Conformations II. Solution Structure Refinement Of Brazzein Chemistry. PhD thesis, University of Wisconsin, Madison, WI.
11. Gao, G.H., Dai, JX, Ding, M., Hellekant, G., Wang, J.F. and Wang, D.C. (1999) Solution conformation of brazzein by 1H nuclear magnetic resonance: resonance assignment and secondary structure. Int. J. Biol. Macromol., 24, 351-359.
12. Green, B.G., Dalton, P., Cowart, B., Shaffer, G., Rankin, K. and Higgins, J. (1996) Evaluating the 'Labeled Magnitude Scale' for measuring sensations of taste and smell. Chem. Senses, 21, 323-334.
13. Hellekant, G. (1994) The physiology of thaumatin. In Whitty, M. and Higginbotham, J.D. (eds), Thaumatin. CRC Press, Boca Raton, FL, pp. 99-113.
14. Hellekant, G. and Roberts, T.W. (1995) Whole nerve and single fiber recordings in non-human primates. In Spielman, A.I. and Brand, J.G. (eds), Experimental Cell Biology of Taste and Olfaction: Current Techniques and Protocols. CRC Press, Boca Raton, FL, pp. 277-290.
15. Hellekant, G., van der Wel, H., Brouwer, J.N., Glaser, D., Segerstad, C.H. and Roberts, T.W. (1986) Chorda tympani proper nerve responses to intra-arterial and surface stimulation of the tongue in rhesus monkey and rat. Chem. Senses, 11, 557-566.
16. Hellekant, G., Danilova, V. and Ninomiya, Y. (1997a) Primate sense of taste: behavioral and single chorda tympani and glossopharyngeal nerve fiber recordings in the rhesus monkey, Macaca mulatta. J. Neurophysiol., 77, 978-993.
17. Hellekant, G., Ninomiya, Y. and Danilova, V. (1997b) Taste in chimpanzees II: single chorda tympani fibers. Physiol. Behav., 61, 829-841.
18. Hellekant, G., Ninomiya, Y. and Danilova, V. (1998) Taste in chimpanzees III: Labeled line coding in sweet taste. Physiol. Behav., 65.
19. Hladik, A., Bahuchet, S., Ducatillion, C. and Hladik, E.M. (1984) Les plantes a tubercules de la foret dense d'afrique centrale. Rev. Ecol. (Terre Vie), 39, 249-290.
20. Ishikawa, K., Ota, M., Ariyoshi, Y., Sasaki, H., Tanokura, M., Ding, M., Caldwell, J. and Abildgaad, F. (1996) Crystallization and preliminary X-ray analysis of brazzein, a new sweet protein. Acta Crystallogr. D Biol. Crystallogr., 52, 577-578.
21. Kaneko, R. and Kitabatake, N. (2001) Structure-sweetness relationship in thaumatin: importance of lysine residues. Chem. Senses, 26, 167-177.
22. Kohmura, M., Ota, M., Izawa, H., Ming, D., Hellekant, G. and Ariyoshi, Y. (1996) Assignment of the disulfide bonds in the sweet protein brazzein. Biopolymers, 38, 553-556.
23. Li, X., Staszewski, L., Xu, H., Durick, K., Zoller, M. and Adler, E. (2002) Human receptors for sweet and umami taste. Proc. Natl Acad. Sci. USA, 99, 4692-4696.
24. Ming, D. (1994) Brazzein, a New Sweet Protein from Pentadiplandra brazzeana. PhD thesis, University of Wisconsin at Madison.
25. Ming, D. and Hellekant, G. (1994) Brazzein, a new high-potency thermostable sweet protein from Pentadiplandra brazzeana B. FEBS Lett., 355, 106-108.
26. Nelson, G., Hoon, M.A., Chandrashekar, J., Zhang, Y., Ryba, N.J. and Zuker, C.S. (2001) Mammalian sweet taste receptors. Cell, 106, 381-390.
27. Temussi, P. (2002) Why are sweet proteins sweet? Interaction of brazzein, monellin and thaumatin with the T1R2-T1R3 receptor. FEBS Lett., 526, 1.