Alcohol and PRAS40 knockdown decrease mTOR activity and protein synthesis via AMPK signaling and changes in mTORC1 interaction

Journal of Cellular Biochemistry

Volumn 109, Issue 6, 2010, Pages 1172-1184

  Hong Brown, Ly Q ^a   Brown, C Randell ^a   Kazi, Abid A ^a   Huber, Danuta S ^a   Pruznak, Anne M ^a   Lang, Charles H ^a  

^a PENN STATE COLLEGE OF MEDICINE   (United States)

Author keywords

AMPK; mTORC1; Myocytes; PRAS40

Indexed keywords

ADENYLATE KINASE; ALCOHOL; MAMMALIAN TARGET OF RAPAMYCIN; MTORC1 PROTEIN; PRAS40 PROTEIN; PROTEIN 14 3 3; RAPTOR PROTEIN; SHORT HAIRPIN RNA; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME PHOSPHORYLATION; IMMUNOBLOTTING; IMMUNOPRECIPITATION; MOUSE; MUSCLE CELL; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN SYNTHESIS;

AMP-ACTIVATED PROTEIN KINASES; ANIMALS; CARRIER PROTEINS; CELL LINE; ETHANOL; GENE EXPRESSION REGULATION; IMMUNOBLOTTING; IMMUNOPRECIPITATION; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; MICE; MUSCLE CELLS; PHOSPHOPROTEINS; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN-SERINE-THREONINE KINASES; RNA INTERFERENCE; SIGNAL TRANSDUCTION; TRANSCRIPTION FACTORS;

RAPTORES;

EID: 77951728579     PISSN: 07302312     EISSN: 10974644     Source Type: Journal    
DOI: 10.1002/jcb.22496     Document Type: Article

References (40)

1. Andrabi S, Gjoerup OV, Kean JA, Roberts TM, Schaffhausen B. 2007. Protein phosphatase 2A regulates life and death decisions via Akt in a context-dependent manner. Proc Natl Acad Sci USA 104:19011-19016.
2. Avruch J, Long X, Ortiz-Vega S, Rapley J, Papageorgiou A, Dai N. 2009. Amino acid regulation of TOR complex 1. Am J Physiol Endocrinol Metab 296:E592-E602.
3. Bridges D, Moorhead GB. 2005. 14-3-3 proteins: A number of functions for a numbered protein. Sci STKE 296:re10.
4. Fonseca BD, Smith EM, Lee VH, MacKintosh C, Proud CG. 2007. PRAS40 is a target for mammalian target of rapamycin complex 1 and is required for signaling downstream of this complex. J Biol Chem 282:24514-24524. (Pubitemid 47347551)
5. Fonseca BD, Lee VH, Proud CG. 2008. The binding of PRAS40 to 14-3-3 proteins is not required for activation of mTORC1 signalling by phorbol esters/ERK. Biochem J 411:141-149.
6. Fujita Y, Hiroyama M, Sanbe A, Yamauchi J, Murase S, Tanoue A. 2008. ETOH inhibits embryonic neural stem/precursor cell proliferation via PLD signaling. Biochem Biophys Res Commun 370:169-173.
7. Guertin DA, Stevens DM, Thoreen CC, Burds AA, Kalaany NY, Moffat J, Brown M, Fitzgerald KJ, Sabatini DM. 2006. Ablation in mice of the mTORC components raptor, rictor, or mLST8 reveals that mTORC2 is required for signaling to Akt-FOXO and PKCalpha, but not S6K1. Dev Cell 11:859-871. (Pubitemid 44804279)
8. Gwinn DM, Shackelford DB, Egan DF, Mihaylova MM, Mery A, Vasquez DS, Turk BE, Shaw RJ. 2008. AMPK phosphorylation of raptor mediates a metabolic checkpoint. Mol Cell 30:214-226. (Pubitemid 351626684)
9. Hara K, Maruki Y, Long X, Yoshino K, Oshiro N, Hidayat S, Tokunaga C, Avruch J, Yonezawa K. 2002. Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action. Cell 110:177-189. (Pubitemid 34876546)
10. Hong-Brown LQ, Frost RA, Lang CH. 2001. Alcohol impairs protein synthesis and degradation in cultured skeletal muscle cells. Alcohol Clin Exp Res 25: 1373-1382.
11. Hong-Brown LQ, Brown CR, Huber DS, Lang CH. 2006. Alcohol and indinavir adversely affect protein synthesis and phosphorylation of MAPK and mTOR signaling pathways in C2C12 myocytes. Alcohol Clin Exp Res 30:1297-1307.
12. Hong-Brown LQ, Brown CR, Huber DS, Lang CH. 2007. Alcohol regulates eukaryotic elongation factor 2 phosphorylation via an AMP-activated protein kinase-dependent mechanism in C2C12 skeletal myocytes. J Biol Chem 282:3702-3712.
13. Hong-Brown LQ, Brown CR, Huber DS, Lang CH. 2008. Lopinavir impairs protein synthesis and induces eEF2 phosphorylation via the activation of AMP-activated protein kinase. J Cell Biochem 105:814-823.
14. Inoki K, Li Y, Zhu T, Wu J, Guan KL. 2002. TSC2 is phosphorylated and inhibited by Akt and suppresses mTOR signalling. Nat Cell Biol 4:648-657. (Pubitemid 34993700)
15. Inoki K, Zhu T, Guan KL. 2003. TSC2 mediates cellular energy response to control cell growth and survival. Cell 115:577-590. (Pubitemid 37506046)
16. Jacinto E, Loewith R, Schmidt A, Lin S, Ruegg MA, Hall A, Hall MN. 2004. Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive. Nat Cell Biol 6:1122-1128. (Pubitemid 39468014)
17. Kim DH, Sarbassov DD, Ali SM, King JE, Latek RR, Erdjument-Bromage H, Tempst P, Sabatini DM. 2002. mTOR interacts with raptor to form a nutrient-sensitive complex that signals to the cell growth machinery. Cell 110:163-175. (Pubitemid 34876545)
18. Kim DH, Sarbassov DD, Ali SM, Latek RR, Guntur KV, Erdjument-Bromage H, Tempst P, Sabatini DM. 2003. GbetaL, a positive regulator of the rapamycin-sensitive pathway required for the nutrient-sensitive interaction between raptor and mTOR. Mol Cell 11:895-904. (Pubitemid 36566312)
19. Kovacina KS, Park GY, Bae SS, Guzzetta AW, Schaefer E, Birnbaum MJ, Roth RA. 2003. Identification of a proline-rich Akt substrate as a 14-3-3 binding partner. J Biol Chem 278:10189-10194. (Pubitemid 36800278)
20. Loewith R, Jacinto E, Wullschleger S, Lorberg A, Crespo JL, Bonenfant D, Oppliger W, Jenoe P, Hall MN. 2002. Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control. Mol Cell 10:457-468. (Pubitemid 35284167)
21. Manning BD, Tee AR, Logsdon MN, Blenis J, Cantley LC. 2002. Identification of the tuberous sclerosis complex-2 tumor suppressor gene product tuberin as a target of the phosphoinositide 3-kinase/akt pathway. Mol Cell 10:151-162. (Pubitemid 34876569)
22. Memmott RM, Dennis PA. 2009. Akt-dependent and -independent mechanisms of mTOR regulation in cancer. Cell Signal 21:656-664.
23. Miller AM, Brestoff JR, Phelps CB, Berk EZ, Reynolds THt. 2008. Rapamycin does not improve insulin sensitivity despite elevated mammalian target of rapamycin complex 1 activity in muscles of ob/ob mice. Am J Physiol Regul Integr Comp Physiol 295:R1431-R1438.
24. Nascimento EB, Fodor M, van der Zon GC, Jazet IM, Meinders AE, Voshol PJ, Vlasblom R, Baan B, Eckel J, Maassen JA, Diamant M, Ouwens DM. 2006. Insulin-mediated phosphorylation of the proline-rich Akt substrate PRAS40 is impaired in insulin target tissues of high-fat diet-fed rats. Diabetes 55: 3221-3228. (Pubitemid 44923577)
25. Oshiro N, Takahashi R, Yoshino K, Tanimura K, Nakashima A, Eguchi S, Miyamoto T, Hara K, Takehana K, Avruch J, Kikkawa U, Yonezawa K. 2007. The proline-rich Akt substrate of 40 kDa (PRAS40) is a physiological substrate of mammalian target of rapamycin complex 1. J Biol Chem 282: 20329-20339. (Pubitemid 47100038)
26. Sancak Y, Thoreen CC, Peterson TR, Lindquist RA, Kang SA, Spooner E, Carr SA, Sabatini DM. 2007. PRAS40 is an insulin-regulated inhibitor of the mTORC1 protein kinase. Mol Cell 25:903-915. (Pubitemid 46436534)
27. Sarbassov DD, Ali SM, Kim DH, Guertin DA, Latek RR, Erdjument-Bromage H, Tempst P, Sabatini DM. 2004. Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Curr Biol 14:1296-1302. (Pubitemid 38991819)
28. Sarbassov DD, Ali SM, Sabatini DM. 2005a. Growing roles for the mTOR pathway. Curr Opin Cell Biol 17:596-603.
29. Sarbassov DD, Guertin DA, Ali SM, Sabatini DM. 2005b. Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Science 307:1098-1101.
30. Sato T, Nakashima A, Guo L, Tamanoi F. 2009. Specific activation of mTORC1 by Rheb G-protein in vitro involves enhanced recruitment of its substrate protein. J Biol Chem 284:12783-12791.
31. Smith EM, Finn SG, Tee AR, Browne GJ, Proud CG. 2005. The tuberous sclerosis protein TSC2 is not required for the regulation of the mammalian target of rapamycin by amino acids and certain cellular stressors. J Biol Chem 280:18717-18727.
32. Vander Haar E, Lee SI, Bandhakavi S, Griffin TJ, Kim DH. 2007. Insulin signalling to mTOR mediated by the Akt/PKB substrate PRAS40. Nat Cell Biol 9:316-323. (Pubitemid 46344611)
33. Wang L, Harris TE, Roth RA, Lawrence JC, Jr. 2007. PRAS40 regulates mTORC1 kinase activity by functioning as a direct inhibitor of substrate binding. J Biol Chem 282:20036-20044. (Pubitemid 47100127)
34. Wang L, Harris TE, Lawrence JC, Jr. 2008. Regulation of proline-rich Akt substrate of 40 kDa (PRAS40) function by mammalian target of rapamycin complex 1 (mTORC1)-mediated phosphorylation. J Biol Chem 283:15619-15627.
35. Wullschleger S, Loewith R, Oppliger W, Hall MN. 2005. Molecular organization of target of rapamycin complex 2. J Biol Chem 280:30697-33704.
36. Wullschleger S, Loewith R, Hall MN. 2006. TOR signaling in growth and metabolism. Cell 124:471-484.
37. Yaffe MB. 2002. How do 14-3-3 proteins work? - Gatekeeper phosphorylation and the molecular anvil hypothesis. FEBS Lett 513:53-57.
38. Yu F, Narasimhan P, Saito A, Liu J, Chan PH. 2008. Increased expression of a proline-rich Akt substrate (PRAS40) in human copper/zinc-superoxide dismutase transgenic rats protects motor neurons from death after spinal cord injury. J Cereb Blood Flow Metab 28:44-52.
39. Zhang F, Beharry ZM, Harris TE, Lilly MB, Smith CD, Mahajan S, Kraft AS. 2009a. PIM1 protein kinase regulates PRAS40 phosphorylation and mTOR activity in FDCP1 cells. Cancer Biol Ther 8:846-853.
40. Zhang L, Kimball SR, Jefferson LS, Shenberger JS. 2009b. Hydrogen peroxide impairs insulin-stimulated assembly of mTORC1. Free Radic Biol Med 46: 1500-1509.