Evaluation of the protective effect of ascorbic acid on nitrite- and nitrosamine-induced cytotoxicity and genotoxicity in human hepatoma line

Toxicology Mechanisms and Methods

Volumn 20, Issue 2, 2010, Pages 45-52

  Erkekoglu, Pinar ^a   Baydar, Terken ^a  

^a HACETTEPE UNIVERSITY   (Turkey)

Author keywords

Ascorbic acid; Comet; Genotoxicity; HepG2; MTT; Nitrite; Nitrosamine

Indexed keywords

ASCORBIC ACID; DIETHYLNITROSAMINE; DIMETHYLNITROSAMINE; NITRITE; NITROSAMINE; NITROSOMORPHOLINE; REACTIVE OXYGEN METABOLITE;

ARTICLE; CELL VIABILITY; COMET ASSAY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY; DRUG ACTIVITY; GENOTOXICITY; HUMAN; HUMAN CELL; LIVER CELL CARCINOMA; PRIORITY JOURNAL;

ANTIOXIDANTS; ASCORBIC ACID; CARCINOMA, HEPATOCELLULAR; CELL SURVIVAL; COMET ASSAY; CYTOPROTECTION; DIETHYLNITROSAMINE; DIMETHYLNITROSAMINE; DNA DAMAGE; DOSE-RESPONSE RELATIONSHIP, DRUG; HEP G2 CELLS; HUMANS; LIVER NEOPLASMS; MUTAGENS; NITRITES; NITROSAMINES; OXIDATIVE STRESS; REACTIVE OXYGEN SPECIES; TIME FACTORS;

NICOTIANA TABACUM;

EID: 77149138693     PISSN: 15376516     EISSN: 15376524     Source Type: Journal    
DOI: 10.3109/15376510903583711     Document Type: Article

References (53)

1. Abnet CC. 2007. Carcinogenic food contaminants. Cancer Invest 25:189-196.
2. Ames BN. 1999. Micronutrient deficiencies. A major cause of DNA damage. Ann NY Acad Sci 889:87-106.
3. Appel KE, Graf H. 1982. Metabolic nitrite formation from N-nitrosamines: evidence for a cytochrome P-450 dependent reaction. Carcinogenesis 3:293-296.
4. Archer MC. 1989. Mechanisms of action of N-nitroso compounds. Cancer Surv 8:241-250.
5. Arranz N, Haza AI, García A, Möller L, Rafter J, Morales P. 2006. Protective effects of isothiocyanates towards N-nitrosamine-induced DNA damage in the single-cell gel electrophoresis (SCGE)/HepG2 assay. J Appl Toxicol 26:493-499.
6. Arranz N, Haza AI, García A, Möller L, Rafter J, Morales P. 2007. Protective effects of organosulfur compounds towards N-nitrosamine-induced DNA damage in the single-cell gel electrophoresis (SCGE)/HepG2 assay. Food Chem Toxicol 45:1662-1669.
7. Athar M. 2002. Oxidative stress and experimental carcinogenesis. Indian J Exp Biol 40: 656-667.
8. Barnham KJ, Masters CL, Bush AI. 2004. Neurodegenerative diseases and oxidative stress. Nat Rev Drug Dis cov 3:205-214.
9. Bartsch H, Spiegelhalder B. 1996. Environmental exposure to N-nitroso compounds (NNOC) and precursors: an overview. Eur J Cancer Prev 5:11-17.
10. Bast A, van der Plas RM, van den Berg H, Haenen GR. 1996. Beta-carotene as antioxidant. Eur J Clin Nutr 50:S54-S56.
11. Boffetta P, Hecht S, Gray N, Gupta P, Straif K. 2008. Smokeless tobacco and cancer. Lancet Oncol 9:667-675.
12. Bruning-Fann CS, Kaneene JB. 1993a. The effects of nitrate, nitrite, and N-nitroso compounds on animal health. Vet Hum Toxicol 35:237-253.
13. Bruning-Fann CS, Kaneene JB. 1993b. The effects of nitrate, nitrite and N-nitroso compounds on human health: a review, Vet Hum Toxicol 35:521-538.
14. Castenmiller JJ, Lauridsen ST, Dragsted LO, van het Hof KH, Linssen J, West CE. 1999. Beta-carotene does not change markers of enzymatic and nonenzymatic antioxidant activity in human blood. J Nutr 129:2162-2169.
15. Chaudière J, Ferrari-Iliou R. 1999 Intracellular antioxidants: from chemical to biochemical mechanisms. Food Chem Toxicol 37:949-962.
16. Chow CK, Hong CB. 2002. Dietary vitamin E and selenium and toxicity of nitrite and nitrate. Toxicology 180:195-207.
17. Chung SY, Kim JS, Kim M, Hong MK, Lee JO, Kim CM, Song IS. 2003. Survey of nitrate and nitrite contents of vegetables grown in Korea. Food Ad dit Contam 20:621-628.
18. Claycombe KJ, Meydani SN. 2001. Vitamin E and genome stability. Mutat Res 475:37-44.
19. Gamet-Payrastre L, Li P, Lumeau S, Cassar G, Dupont MA, Chevolleau S, Gasc N, Tulliez J, Tercé F. 2000. Sulforaphane, a naturally occurring isothiocyanate, induces cell cycle arrest and apoptosis in HT29 human colon cancer cells. Cancer Res 60:1426-1433.
20. Garcia A, Haza AI, Arranz N, Delgado ME, Rafter J, Morales P. 2008a. Organosulfur compounds alone or in combination with vitamin C protect towards N-nitrosopiperidine- and N-nitrosodibutylamine-induced oxidative DNA damage in HepG2 cells. Chem Biol Interact 173:9-18.
21. Garcia A, Haza AI, Arranz N, Rafter J, Morales P. 2008b. Protective effects of isothiocyanates alone or in combination with vitamin C towards N-nitrosodibutylamine or N-nitrosopiperidine-induced oxidative DNA damage in the single-cell gel electrophoresis (SCGE)/HepG2 assay. J Appl Toxicol 28:196-204.
22. George J, Rao KR, Stern R, Chandrakasan G. 2001. Dimethylnitrosamine- induced liver injury in rats: the early deposition of collagen. Toxicology 156:129-138.
23. Gichner T, Velemínský J. 1988. Inhibitors of N-nitroso compounds-induced mutagenicity. Mutat Res 195:21-43.
24. Halliwell B. 2001. Vitamin C and genomic stability. Mutat Res 475:29-35.
25. Halliwell B, Cross CE. 1994. Oxygen-derived species: their relation to human disease and environmental stress. Environ Health Perspect 102:5-12.
26. Hininger I, Chollat-Namy A, Osman M, Arnaud J, Ducros V, Favier A, Roussel AM. 2002. Beneficial effect of an antioxidant micronutrient-enriched food on DNA damage: experimental study in rats using a modified comet assay in total blood. IARC Sci Publ 156:395-396.
27. IARC (International Agency for Research on Cancer). 2000. Some industrial chemicals. Vol. 77.15-22 February 2000. Available online at: http://monographs.iarc.fr. Accessed 20 November 2009.
28. INCHEM (International Programme on Chemical Safety). 1996. Environmental Health Criteria 179. Morpholine. Available online at: www.inchem.org/documents/ ehc/ehc/ehcl79.htm. Accessed 20 November 2009.
29. Jakszyn P, Gonzalez CA. 2006. Nitrosamine and related food intake and gastric and oesophageal cancer risk: a systematic review of the epidemiological evidence. World J Gastroenterol 12:4296-4303.
30. Jose K, Kuttan R, Bhattacharya K. 1998. Effect of Emblica officinalis extract on hepatocarcinogenesis and carcinogen metabolism. J Clin Biochem Nutr 25:31-39.
31. Kamataki T, Fujita K, Nakayama K, Yamazaki Y, Miyamoto M, Ariyoshi N. 2002. Role of human cytochrome P450 (CYP) in the metabolic activation of nitrosamine derivatives: application of genetically engineered Salmonella expressing human CYP. Drug Metab Rev 34:667-676.
32. Kasprzyk-Hordern B, Andrzejewski P, Nawrocki J. 2005. The hazard of N-nitrosodimethylamine (NDMA) formation during water disinfection with strong oxidants. Desalination 176:37-45.
33. Liao DJ, Blanck A, Eneroth P, Gustafsson JA, Hällström IP. 2001. Diethylnitrosamine causes pituitary damage, disturbs hormone levels, and reduces sexual dimorphism of certain liver functions in the rat. Environ Health Perspect 109:943-947.
34. Loikkanen JJ, Naarala J, Savolainen KM. 1998. Modification of glutamate-induced oxidative stress by lead: the role of extracellular calcium. Free Radic Biol Med 24:377-384.
35. McKnight GM, Duncan CW, Leifert C, Golden MH. 1999. Dietary nitrate in man: friend or foe? Br J Nutr 81:349-358.
36. McMullen SE, Casanova JA, Gross LK, Schenck FJ. 2005. Ion chromatographic determination of nitrate and nitrite in vegetable and fruit baby foods. J AOAC Int 88:1793-1796.
37. Peto R, Gray R, Brantom P, Grasso P. 1991. Dose and time relationships for tumor induction in the liver and esophagus of 4080 inbred rats by chronic ingestion of N-nitrosodiethylamine or N-nitrosodimethylamine. Cancer Res 51:6452-6469.
38. Platz EA, Willett WC, Colditz GA, Rimm EB, Spiegelman D, Giovannucci E. 2000. Proportion of colon cancer risk that might be preventable in a cohort of middle-aged US men. Cancer Causes Control 11:579-588.
39. Robichová S, Slamenová D. 2002. Effects of vitamins C and E on cytotoxicity induced by N-nitroso compounds, N-nitrosomorpholine and N-methyl-N′-nitro-N-nitrosoguanidine in Caco-2 and V79 cell lines. Cancer Lett 182:11-18.
40. Robichová S, Slamenová D, Chalupa I, Sebová L. 2004b. DNA lesions and cytogenetic changes induced by N-nitrosomorpholine in HepG2, V79 and VH10 cells: the protective effects of Vitamins A, C and E. Mutat Res 560:91-99.
41. Robichová S, Slamenová D, Gábelová A, Sedlák J, Jakubíková J. 2004a. An investigation of the genotoxic effects of N-nitrosomorpholine in mammalian cells. Chem Biol Interact 148:163-171.
42. Roomi MW, Netke S, Tsao C. 1998. Modulation of drug metabolizing enzymes in guinea pig liver by high intakes of ascorbic acid. Int J Vitam Nutr Res 68:42-47.
43. Schorah C. 1999. Micronutrients, vitamins, and cancer risk. Vitam Horm 57:1-23.
44. Surh YJ. 2002. Anti-tumor promoting potential of selected spice ingredients with antioxidative and anti-inflammatory activities: a short review. Food Chem Toxicol 40:1091-1097.
45. Surh YJ, Chun KS, Cha HH, Han SS, Keum YS, Park KK, Lee SS. 2001. Molecular mechanisms underlying chemopreventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-kappa B activation. Mutat Res 480-481:243-268.
46. Tanaka K, Hayatsu T, Negishi T, Hayatsu H. 1998. Inhibition of N-nitrosation of secondary amines in vitro by tea extracts and catechins. Mutat Res 412:91-98.
47. Tricker AR. 1997. N-nitroso compounds and man: sources of exposure, endogenous formation and occurrence in body fluids. Eur J Cancer Prev 6:226-268.
48. Tricker AR, Preussmann R. 1991. Carcinogenic N-nitrosamines in the diet: occurrence, formation, mechanisms and carcinogenic potential. Mutat Res 259:277-289.
49. Valko M, Izakovic M, Mazur M, Rhodes CJ, Telser J. 2004. Role of oxygen radicals in DNA damage and cancer incidence. Mol Cell Biochem 266:37-56.
50. Vallejo F, Tomás-Barberán FA, García-Viguera C. 2002. Potential bioactive compounds in health promotion from broccoli cultivars grown in Spain. J Sci Food Agric 82:1293-1297.
51. Vitaglione P, Morisco F, Caporaso N, Fogliano V. 2004. Dietary antioxidant compounds and liver health. Crit Rev Food Sci Nutr 44:575-586.
52. Wang W, Higuchi CM. 1995. Induction of NAD(P)H:quinone reductase by vitamins A, E and C in Colo205 colon cancer cells. Cancer Lett 98:63-69.
53. Willett WC. 2001. Diet and cancer: one view at the start of the millennium. Cancer Epidemiol Biomarkers Prev 10:3-8.