Isomerization in the CDR2 of a monoclonal antibody: Binding analysis and factors that influence the isomerization rate

Biotechnology and Bioengineering

Volumn 105, Issue 3, 2010, Pages 515-523

  Dick Jr , Lawrence W ^a   Qiu, Difei ^a   Wong, Rosie B ^a   Cheng, Kuang Chuan ^a  

^a MEDAREX INC   (United States)

Author keywords

Aspartic acid; Complementarity determining region; Hydrophobic interaction chromatography; Isomerization; Monoclonal antibody

Indexed keywords

ASPARTIC ACIDS; BINDING ANALYSIS; BINDING CONSTANT; BUFFER COMPONENTS; COMPLEMENTARITY-DETERMINING REGIONS; HEAVY CHAIN; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; NEGATIVE CHARGE; PROTEIN DEGRADATION; PROTON ACCEPTORS; REAL SAMPLES; THERAPEUTIC ANTIBODIES;

ARRHENIUS PLOTS; CHROMATOGRAPHIC ANALYSIS; ELECTRON TRANSITIONS; HYDROPHOBICITY; ISOMERIZATION; ISOMERS; MONOCLONAL ANTIBODIES; ORGANIC ACIDS; RATE CONSTANTS; SURFACE PLASMON RESONANCE;

HYDROPHOBIC CHROMATOGRAPHY;

BUFFER; ISOASPARTIC ACID; MONOCLONAL ANTIBODY; PROTON;

ARTICLE; BINDING AFFINITY; CHROMATOGRAPHY; COMPLEMENTARITY DETERMINING REGION; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; ISOMERIZATION; PH; TEMPERATURE;

ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; BUFFERS; CHROMATOGRAPHY, LIQUID; COMPLEMENTARITY DETERMINING REGIONS; HYDROGEN-ION CONCENTRATION; HYDROPHOBICITY; ISOMERISM; PROTEIN BINDING;

EID: 74849124598     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22561     Document Type: Article

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