Characterization of asparagine deamidation and aspartate isomerization in recombinant human interleukin-11

Pharmaceutical Research

Volumn 19, Issue 8, 2002, Pages 1223-1231

  Zhang, Wei ^a   Czupryn, Marta J ^a   Boyle, Philip T ^a   Amari, John ^a  

^a PFIZER INC   (United States)

Author keywords

Deamidation; Interleukin 11; Isoaspartate; Isomerization

Indexed keywords

ASPARAGINE; ASPARTIC ACID; PROTEINASE; RECOMBINANT INTERLEUKIN 11; TRYPSIN;

AMINO ACID COMPOSITION; ANIMAL CELL; ARTICLE; CAPILLARY ELECTROPHORESIS; CONTROLLED STUDY; DEAMINATION; DRUG ACTIVITY; HEAT STRESS; ISOMERISM; LIQUID; MASS SPECTROMETRY; MOUSE; NONHUMAN; PEPTIDE MAPPING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN MODIFICATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; TEMPERATURE SENSITIVITY;

AMIDES; AMINO ACID SEQUENCE; ASPARAGINE; ASPARTIC ACID; HUMANS; INTERLEUKIN-11; ISOMERISM; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, PROTEIN;

EID: 0036692454     PISSN: 07248741     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1019814713428     Document Type: Article

References (27)

1. Stability of protein pharmaceuticals
2. The development of stable protein formulations: A close look at protein aggregation, deamidation, and oxidation
3. Deamidation: A source of miscroheterogeneity in pharmaceutical proteins
4. Isoaspartate in peptides and proteins: Formation, significance, and analysis
5. Deamidation, isomerization, and racemization at asparaginyl and aspartyl residues in peptides - Succinimide-linked reactions that contribute to protein degradation
6. Protein carboxyl methyltransferase facilitates conversion of atypical L-isoaspartyl peptides to normal L-aspartyl peptides
7. Succinimide formation from aspartyl and asparaginyl peptides as a model for the spontaneous degradation of proteins
8. Tertiary structure is a principal determination to protein deamidation
9. Effects of amino acid sequences, buffers, and ionic strength on the rate and mechanism of deamidation of asparagine residus in small peptides
10. Secondary structure and protein deamidation
11. Structure-function relationships in human interleukin-11
12. Alanine-scanning mutagenesis of human interleukin-11: Identification of regions important for biologic activity
13. Development of improved high-performance liquid chromatography conditions for nonisotopic detection of isoaspartic acid to determine the extent of protein deamidation
14. Molecular cloning of a cDNA encoding interleukin 11, a stromal cell-derived lymphopoietic and hematopoietic cytokine
15. Peracetic acid oxidation of rhIL-11
16. Origin of the isoelectric heterogeneity of monoclonal immunoglobulin h1B4
17. Determination of the origin of charge heterogeneity in a murine monoclonal antibody
18. Isomerization of an aspartic acid residue in the complementarity-determining regions of a recombinant antibody to human IgE: Identification and effect on binding affinity
19. Selective deamidation of recombinant human stem cell factor during in vitro aging: Isolation and characterization of the aspartyl and isoaspartyl homodimers and heterodimers
20. Identification of multiple sources of charge heterogeneity in a recombinant antibody
21. Deficiency of a protein-repair enzyme results in the accumulation of altered proteins, retardation of growth, and fatal seizures in mice
22. Isoaspartyl post-translational modification triggers autoimmune responses to self-proteins
23. Crystal structure of Ustilago sphaerogena ribonuclease U2 at 1.8 Å resolution
24. Succinimide and isoaspartate residues in the crystal structures of hen egg-white lysozyme complexed with tri-N-acetylchitotriose