Targeted deletion of autophagy-related 5 (atg5) impairs adipogenesis in a cellular model and in mice

Autophagy

Volumn 5, Issue 8, 2009, Pages 1118-1130

  Baerga, Rebecca ^a   Zhang, Yong ^a   Chen, Po Hao ^a   Goldman, Scott ^a   Jin, Shengkan ^a  

^a RUTGERS ROBERT WOOD JOHNSON MEDICAL SCHOOL   (United States)

Author keywords

Adipocyte differentiation; Adipogenesis; Atg5; Autophagy; WAT

Indexed keywords

AUTOPHAGY PROTEIN 5; CHLOROQUINE; MEMBRANE PROTEIN; PERILIPIN;

ADIPOCYTE; ADIPOGENESIS; ANIMAL CELL; ANIMAL TISSUE; APOPTOSIS; ARTICLE; AUTOPHAGY; CELL ACTIVATION; CELL DIFFERENTIATION; CONTROLLED STUDY; DRUG MECHANISM; EMBRYO; EMBRYO CELL; EXPERIMENTAL MODEL; FIBROBLAST; GENE DELETION; GENE EXPRESSION; HISTOLOGY; MOUSE; NEWBORN; NONHUMAN; SUBCUTANEOUS TISSUE;

MAMMALIA; MUS;

EID: 73449117508     PISSN: 15548627     EISSN: 15548635     Source Type: Journal    
DOI: 10.4161/auto.5.8.9991     Document Type: Article

References (37)

1. Rosen ED, Spiegelman BM. Adipocytes as regulators of energy balance and glucose homeostasis. Nature 2006; 444:847-53.
2. Cannon B, Nedergaard J. Brown adipose tissue: function and physiological significance. Physiol Rev 2004; 84:277-359.
3. Farmer SR. Transcriptional control of adipocyte formation. Cell Metab 2006; 4:263-73.
4. Hansen JB, Kristiansen K. Regulatory circuits controlling white versus brown adipocyte differentiation. Biochem J 2006; 398:153-68.
5. Rosen ED, MacDougald OA. Adipocyte differentiation from the inside out. Nat Rev Mol Cell Biol 2006; 7:885-96.
6. Rosen ED, Walkey CJ, Puigserver P, Spiegelman BM. Transcriptional regulation of adipogenesis. Genes Dev 2000; 14:1293-307.
7. Chen PL, Riley DJ, Chen Y, Lee WH. Retinoblastoma protein positively regulates terminal adipocyte differentiation through direct interaction with C/EBPs. Genes Dev 1996; 10:2794-804.
8. Kubota N, Terauchi Y, Miki H, et al. PPARgamma mediates high-fat diet-induced adipocyte hypertrophy and insulin resistance. Mol Cell 1999; 4:597-609.
9. Sue N, Jack BH, Eaton SA, et al. Targeted disruption of the basic Kruppel-like factor gene (Klf3) reveals a role in adipogenesis. Mol Cell Biol 2008; 28:3967-78.
10. Wu Z, Rosen ED, Brun R, et al. Cross-regulation of C/EBP alpha and PPARgamma controls the transcriptional pathway of adipogenesis and insulin sensitivity. Mol Cell 1999; 3:151-8.
11. Klionsky DJ. Autophagy: from phenomenology to molecular understanding in less than a decade. Nat Rev Mol Cell Biol 2007; 8:931-7.
12. Levine B, Klionsky DJ. Development by self-digestion: molecular mechanisms and biological functions of autophagy. Dev Cell 2004; 6:463-77.
13. Mizushima N. Autophagy: process and function. Genes Dev 2007; 21:2861-73.
14. Ohsumi Y, Mizushima N. Two ubiquitin-like conjugation systems essential for autophagy. Semin Cell Dev Biol 2004; 15:231-6.
15. Qu X, Yu J, Bhagat G, et al. Promotion of tumorigenesis by heterozygous disruption of the beclin 1 autophagy gene. J Clin Invest 2003; 112:1809-20.
16. Yue Z, Jin S, Yang C, Levine AJ, Heintz N. Beclin 1, an autophagy gene essential for early embryonic development, is a haploinsufficient tumor suppressor. Proc Natl Acad Sci USA 2003; 100:15077-82.
17. Hara T, Nakamura K, Matsui M, et al. Suppression of basal autophagy in neural cells causes neurodegenerative disease in mice. Nature 2006; 441:885-9.
18. Kuma A, Hatano M, Matsui M, et al. The role of autophagy during the early neonatal starvation period. Nature 2004; 432:1032-6.
19. Komatsu M, Waguri S, Chiba T, et al. Loss of autophagy in the central nervous system causes neurodegeneration in mice. Nature 2006; 441:880-4.
20. Komatsu M, Waguri S, Ueno T, et al. Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice. J Cell Biol 2005; 169:425-34.
21. Kundu M, Lindsten T, Yang CY, et al. Ulk1 plays a critical role in the autophagic clearance of mitochondria and ribosomes during reticulocyte maturation. Blood 2008; 112:1493-502.
22. Sandoval H, Thiagarajan P, Dasgupta SK, et al. Essential role for Nix in autophagic maturation of erythroid cells. Nature 2008.
23. Schweers RL, Zhang J, Randall MS, et al. NIX is required for programmed mitochondrial clearance during reticulocyte maturation. Proc Natl Acad Sci USA 2007; 104:19500-5.
24. Shibata M, Yoshimura K, Furuya N, et al. The MAP1-LC3 conjugation system is involved in lipid droplet formation. Biochem Biophys Res Commun 2009; 382:419-23.
25. Singh R, Kaushik S, Wang Y, et al. Autophagy regulates lipid metabolism. Nature 2009; 458:1131-5.
26. Novikoff AB, Novikoff PM, Rosen OM, Rubin CS. Organelle relationships in cultured 3T3-L1 preadipocytes. J Cell Biol 1980; 87:180-96.
27. Mizushima N, Noda T, Yoshimori T, et al. A protein conjugation system essential for autophagy. Nature Sep 24 1998; 395:395-8.
28. Mizushima N, Ohsumi Y, Yoshimori T. Autophagosome formation in mammalian cells. Cell Struct Funct 2002; 27:421-9.
29. Banks AS, Li J, McKeag L, et al. Deletion of SOCS7 leads to enhanced insulin action and enlarged islets of Langerhans. J Clin Invest 2005; 115:2462-71.
30. Mizushima N. Methods for monitoring autophagy. Int J Biochem Cell Biol 2004; 36:2491-502.
31. Amaravadi RK, Thompson CB. The roles of therapyinduced autophagy and necrosis in cancer treatment. Clin Cancer Res 2007; 13:7271-9.
32. Amaravadi RK, Yu D, Lum JJ, et al. Autophagy inhibition enhances therapy-induced apoptosis in a Myc-induced model of lymphoma. J Clin Invest 2007; 117:326-36.
33. Spalding KL, Arner E, Westermark PO, et al. Dynamics of fat cell turnover in humans. Nature 2008; 453:783-7.
34. Komatsu M, Wang QJ, Holstein GR, et al. Essential role for autophagy protein Atg7 in the maintenance of axonal homeostasis and the prevention of axonal degeneration. Proc Natl Acad Sci USA 2007; 104:14489-94.
35. DiDonato D, Brasaemle DL. Fixation methods for the study of lipid droplets by immunofluorescence microscopy. J Histochem Cytochem 2003; 51:773-80.
36. Kim YK, Choi HY, Kim NH, et al. Reversine stimulates adipocyte differentiation and downregulates Akt and p70(s6k) signaling pathways in 3T3-L1 cells. Biochem Biophys Res Commun 2007; 358:553-8.
37. Oishi Y, Manabe I, Tobe K, et al. Kruppel-like transcription factor KLF5 is a key regulator of adipocyte differentiation. Cell Metab 2005; 1:27-39.