메뉴 건너뛰기




Volumn 51, Issue 6, 2003, Pages 773-780

Fixation methods for the study of lipid droplets by immunofluorescence microscopy

Author keywords

Adipocytes; Adipophilin; Chemical fixation; Immunofluorescence microscopy; Light microscopy; Lipid droplets; Oil bodies; Oil droplets; Perilipin; Triacylglycerol

Indexed keywords

ACETONE; FAT DROPLET; FIXATIVE; PHOSPHOLIPID;

EID: 0037674997     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215540305100608     Document Type: Article
Times cited : (118)

References (32)
  • 2
    • 11144357274 scopus 로고    scopus 로고
    • Hepatitis C virus core protein shows a cytoplasmic localization and associates to cellular lipid storage droplets
    • Barba G, Harper F, Kohara M, Goulinet S, Matsuura Y, Eder G, Schaff Z, et al. (1997) Hepatitis C virus core protein shows a cytoplasmic localization and associates to cellular lipid storage droplets. Proc Natl Acad Sci USA 94:1200-1205
    • (1997) Proc Natl Acad Sci USA , vol.94 , pp. 1200-1205
    • Barba, G.1    Harper, F.2    Kohara, M.3    Goulinet, S.4    Matsuura, Y.5    Eder, G.6    Schaff, Z.7
  • 4
    • 0030903768 scopus 로고    scopus 로고
    • Post-translational regulation of perilipin expression: Stabilization by stored intracellular neutral lipids
    • Brasaemle DL, Barber T, Kimmel AR, Londos C (1997a) Post-translational regulation of perilipin expression: stabilization by stored intracellular neutral lipids. J Biol Chem 272:9378-9387
    • (1997) J Biol Chem , vol.272 , pp. 9378-9387
    • Brasaemle, D.L.1    Barber, T.2    Kimmel, A.R.3    Londos, C.4
  • 5
    • 0030724392 scopus 로고    scopus 로고
    • Adipose differentiation-related protein is an ubiquitously expressed lipid storage droplet-associated protein
    • Brasaemle DL, Barber T, Wolins NE, Serrero G, Blanchette-Mackie EJ, Londos C (1997b) Adipose differentiation-related protein is an ubiquitously expressed lipid storage droplet-associated protein. J Lipid Res 38:2249-2263
    • (1997) J Lipid Res , vol.38 , pp. 2249-2263
    • Brasaemle, D.L.1    Barber, T.2    Wolins, N.E.3    Serrero, G.4    Blanchette-Mackie, E.J.5    Londos, C.6
  • 6
    • 0033961989 scopus 로고    scopus 로고
    • The lipolytic stimulation of 3T3-L1 adipocytes promotes the translocation of hormone-sensitive lipase to the surface of lipid storage droplets
    • Brasaemle DL, Levin DM, Adler-Wailes DC, Londos C (2000a) The lipolytic stimulation of 3T3-L1 adipocytes promotes the translocation of hormone-sensitive lipase to the surface of lipid storage droplets. Biochim Biophys Acta 1483:251-262
    • (2000) Biochim Biophys Acta , vol.1483 , pp. 251-262
    • Brasaemle, D.L.1    Levin, D.M.2    Adler-Wailes, D.C.3    Londos, C.4
  • 7
    • 0034623950 scopus 로고    scopus 로고
    • Perilipin A increases triacylglycerol storage by decreasing the rate of triacylglycerol hydrolysis
    • Brasaemle DL, Rubin B, Harten IA, Gruia-Gray J, Kimmel AR, Londos C (2000b) Perilipin A increases triacylglycerol storage by decreasing the rate of triacylglycerol hydrolysis. J Biol Chem 275:38486-38493
    • (2000) J Biol Chem , vol.275 , pp. 38486-38493
    • Brasaemle, D.L.1    Rubin, B.2    Harten, I.A.3    Gruia-Gray, J.4    Kimmel, A.R.5    Londos, C.6
  • 8
    • 0034829990 scopus 로고    scopus 로고
    • Possible involvement of protein kinase C in the induction of adipose differentiation-related protein by sterol ester in RAW 264.7 macrophages
    • Chen JS, Greenberg AS, Tseng YZ, Wang SM (2001) Possible involvement of protein kinase C in the induction of adipose differentiation-related protein by sterol ester in RAW 264.7 macrophages. J Cell Biochem 83:187-199
    • (2001) J Cell Biochem , vol.83 , pp. 187-199
    • Chen, J.S.1    Greenberg, A.S.2    Tseng, Y.Z.3    Wang, S.M.4
  • 9
    • 70449158340 scopus 로고
    • A simple method for the isolation and purification of total lipid from animal tissues
    • Folch J, Lees M, Sloane Stanley GH (1957) A simple method for the isolation and purification of total lipid from animal tissues. J Biol Chem 226:497-509
    • (1957) J Biol Chem , vol.226 , pp. 497-509
    • Folch, J.1    Lees, M.2    Sloane Stanley, G.H.3
  • 10
    • 0034724682 scopus 로고    scopus 로고
    • High density lipoprotein-mediated cholesterol uptake and targeting to lipid droplets in intact L-cell fibroblasts: A single- and multiphoton fluorescence approach
    • Frolov A, Petrescu A, Atshaves BP, So PT, Gratton E, Serrero G, Schroeder F (2000) High density lipoprotein-mediated cholesterol uptake and targeting to lipid droplets in intact L-cell fibroblasts: a single- and multiphoton fluorescence approach. J Biol Chem 275:12769-12780
    • (2000) J Biol Chem , vol.275 , pp. 12769-12780
    • Frolov, A.1    Petrescu, A.2    Atshaves, B.P.3    So, P.T.4    Gratton, E.5    Serrero, G.6    Schroeder, F.7
  • 11
    • 0035809931 scopus 로고    scopus 로고
    • Caveolin-2 is targeted to lipid droplets, a new "membrane domain" in the cell
    • Fujimoto T, Kogo H, Ishiguro K, Tauchi K, Nomura R (2001) Caveolin-2 is targeted to lipid droplets, a new "membrane domain" in the cell. J Cell Biol 152:1079-1085
    • (2001) J Cell Biol , vol.152 , pp. 1079-1085
    • Fujimoto, T.1    Kogo, H.2    Ishiguro, K.3    Tauchi, K.4    Nomura, R.5
  • 12
    • 0027937942 scopus 로고
    • Factors underlying the variability of lipid droplet fluorescence in MA-10 Leydig tumor cells
    • Gocze PM, Freeman DA (1994) Factors underlying the variability of lipid droplet fluorescence in MA-10 Leydig tumor cells. Cytometry 17:151-158
    • (1994) Cytometry , vol.17 , pp. 151-158
    • Gocze, P.M.1    Freeman, D.A.2
  • 13
    • 0025772167 scopus 로고
    • Perilipin, a major hormonally regulated adipocyte-specific phosphoprotein associated with the periphery of lipid storage droplets
    • Greenberg AS, Egan JJ, Wek SA, Garry NB, Blanchette-Mackie EJ, Londos C (1991) Perilipin, a major hormonally regulated adipocyte-specific phosphoprotein associated with the periphery of lipid storage droplets. J Biol Chem 266:11341-11346
    • (1991) J Biol Chem , vol.266 , pp. 11341-11346
    • Greenberg, A.S.1    Egan, J.J.2    Wek, S.A.3    Garry, N.B.4    Blanchette-Mackie, E.J.5    Londos, C.6
  • 15
    • 0034493602 scopus 로고    scopus 로고
    • Dynamics of transitional endoplasmic reticulum sites in vertebrate cells
    • Hammond AT, Glick BS (2000) Dynamics of transitional endoplasmic reticulum sites in vertebrate cells. Mol Biol Cell 11:3013-3030
    • (2000) Mol Biol Cell , vol.11 , pp. 3013-3030
    • Hammond, A.T.1    Glick, B.S.2
  • 16
    • 0031733596 scopus 로고    scopus 로고
    • Adipophilin is a specific marker of lipid accumulation in diverse cell types and diseases
    • Heid HW, Moll R, Schwetlick I, Rackwitz HR, Keenan T (1998) Adipophilin is a specific marker of lipid accumulation in diverse cell types and diseases. Cell Tissue Res 294:309-321
    • (1998) Cell Tissue Res , vol.294 , pp. 309-321
    • Heid, H.W.1    Moll, R.2    Schwetlick, I.3    Rackwitz, H.R.4    Keenan, T.5
  • 17
    • 0033880575 scopus 로고    scopus 로고
    • Sequence motifs required for lipid droplet association and protein stability are unique to the hepatitis C virus core protein
    • Hope RG, McLauchlan J (2000) Sequence motifs required for lipid droplet association and protein stability are unique to the hepatitis C virus core protein. J Gen Virol 81:1913-1925
    • (2000) J Gen Virol , vol.81 , pp. 1913-1925
    • Hope, R.G.1    McLauchlan, J.2
  • 18
    • 0026668669 scopus 로고
    • Isolation and characterization of a full-length cDNA coding for an adipose differentiation-related protein
    • Jiang HP, Serrero G (1992) Isolation and characterization of a full-length cDNA coding for an adipose differentiation-related protein. Proc Natl Acad Sci USA 89:7856-7860
    • (1992) Proc Natl Acad Sci USA , vol.89 , pp. 7856-7860
    • Jiang, H.P.1    Serrero, G.2
  • 20
    • 0037200026 scopus 로고    scopus 로고
    • Functional conservation for lipid storage droplet association among perilipin-, ADRP-, and TIP 47-related proteins in mammals, Drosophila, and Dictyostelium
    • Miura S, Gan JW, Brzostowski J, Parisi MJ, Schultz CJ, Londos C, Oliver B, et al. (2002) Functional conservation for lipid storage droplet association among perilipin-, ADRP-, and TIP 47-related proteins in mammals, Drosophila, and Dictyostelium. J Biol Chem 277:32253-32257
    • (2002) J Biol Chem , vol.277 , pp. 32253-32257
    • Miura, S.1    Gan, J.W.2    Brzostowski, J.3    Parisi, M.J.4    Schultz, C.J.5    Londos, C.6    Oliver, B.7
  • 21
    • 0034903123 scopus 로고    scopus 로고
    • The biogenesis and functions of lipid bodies in animals, plants and microorganisms
    • Murphy DJ (2001) The biogenesis and functions of lipid bodies in animals, plants and microorganisms. Prog Lipid Res 40:325-438
    • (2001) Prog Lipid Res , vol.40 , pp. 325-438
    • Murphy, D.J.1
  • 22
    • 0017691245 scopus 로고
    • The display of microtubules in transformed cells
    • Osborn M, Weber K (1977) The display of microtubules in transformed cells. Cell 12:561-571
    • (1977) Cell , vol.12 , pp. 561-571
    • Osborn, M.1    Weber, K.2
  • 23
    • 0035809923 scopus 로고    scopus 로고
    • Accumulation of caveolin in the endoplasmic reticulum redirects the protein to lipid storage droplets
    • Ostermeyer AG, Paci JM, Zeng Y, Lubin DM, Munro S, Brown DA (2001) Accumulation of caveolin in the endoplasmic reticulum redirects the protein to lipid storage droplets. J Cell Biol 152:1071-1078
    • (2001) J Cell Biol , vol.152 , pp. 1071-1078
    • Ostermeyer, A.G.1    Paci, J.M.2    Zeng, Y.3    Lubin, D.M.4    Munro, S.5    Brown, D.A.6
  • 24
    • 0035809933 scopus 로고    scopus 로고
    • A caveolin dominant negative mutant associates with lipid bodies and induces intracellular cholesterol imbalance
    • Pol A, Luetterforst R, Lindsay M, Heino S, Ikonen E, Parton RG (2001) A caveolin dominant negative mutant associates with lipid bodies and induces intracellular cholesterol imbalance. J Cell Biol 152:1057-1070
    • (2001) J Cell Biol , vol.152 , pp. 1057-1070
    • Pol, A.1    Luetterforst, R.2    Lindsay, M.3    Heino, S.4    Ikonen, E.5    Parton, R.G.6
  • 25
    • 0028998905 scopus 로고
    • Perilipins are associated with cholesteryl ester droplets in steroidogenic adrenal cortical and Leydig cells
    • Servetnick DA, Brasaemle DL, Gruia-Gray J, Kimmel AR, Wolff J, Londos C (1995) Perilipins are associated with cholesteryl ester droplets in steroidogenic adrenal cortical and Leydig cells. J Biol Chem 270:16970-16973
    • (1995) J Biol Chem , vol.270 , pp. 16970-16973
    • Servetnick, D.A.1    Brasaemle, D.L.2    Gruia-Gray, J.3    Kimmel, A.R.4    Wolff, J.5    Londos, C.6
  • 27
    • 0032544690 scopus 로고    scopus 로고
    • Overexpression of perilipin A and B blocks the ability of tumor necrosis factor alpha to increase lipolysis in 3T3-L1 adipocytes
    • Souza SC, Moitoso de Vargas L, Yamamoto MT, Lien P, Franciosa MD, Moss LG, Greenberg AS (1998) Overexpression of perilipin A and B blocks the ability of tumor necrosis factor alpha to increase lipolysis in 3T3-L1 adipocytes. J Biol Chem 273:24665-24669
    • (1998) J Biol Chem , vol.273 , pp. 24665-24669
    • Souza, S.C.1    Moitoso de Vargas, L.2    Yamamoto, M.T.3    Lien, P.4    Franciosa, M.D.5    Moss, L.G.6    Greenberg, A.S.7
  • 28
    • 0037040911 scopus 로고    scopus 로고
    • Modulation of hormone-sensitive lipase and protein kinase A-mediated lipolysis by perilipin A in an adenoviral reconstituted system
    • Souza SC, Muliro KV, Liscum L, Lien P, Yamamoto MT, Schaffer JE, Dallal GE, et al. (2002) Modulation of hormone-sensitive lipase and protein kinase A-mediated lipolysis by perilipin A in an adenoviral reconstituted system. J Biol Chem 277:8267-8272
    • (2002) J Biol Chem , vol.277 , pp. 8267-8272
    • Souza, S.C.1    Muliro, K.V.2    Liscum, L.3    Lien, P.4    Yamamoto, M.T.5    Schaffer, J.E.6    Dallal, G.E.7
  • 29
    • 0033165872 scopus 로고    scopus 로고
    • Lipotransin: A novel docking protein for hormone-sensitive lipase
    • Syu LJ, Saltiel AR (1999) Lipotransin: a novel docking protein for hormone-sensitive lipase. Mol Cell 4:109-115
    • (1999) Mol Cell , vol.4 , pp. 109-115
    • Syu, L.J.1    Saltiel, A.R.2
  • 30
    • 14344268795 scopus 로고    scopus 로고
    • Perilipin ablation results in a lean mouse with aberrant adipocyte lipolysis, enhanced leptin production, and resistance to diet-induced obesity
    • Tansey JT, Sztalryd C, Gruia-Gray J, Roush DL, Zee JV, Gavrilova O, Reitman ML, et al. (2001) Perilipin ablation results in a lean mouse with aberrant adipocyte lipolysis, enhanced leptin production, and resistance to diet-induced obesity. Proc Natl Acad Sci USA 98:6494-6499
    • (2001) Proc Natl Acad Sci USA , vol.98 , pp. 6494-6499
    • Tansey, J.T.1    Sztalryd, C.2    Gruia-Gray, J.3    Roush, D.L.4    Zee, J.V.5    Gavrilova, O.6    Reitman, M.L.7
  • 31
    • 0030884256 scopus 로고    scopus 로고
    • Reorganization of a novel vimentin-associated protein in 3T3-L1 cells during adipose conversion
    • Wang SM, Fong T, Hsu S, Chien C, Wu J (1997) Reorganization of a novel vimentin-associated protein in 3T3-L1 cells during adipose conversion. J Cell Biochem 67:84-91
    • (1997) J Cell Biochem , vol.67 , pp. 84-91
    • Wang, S.M.1    Fong, T.2    Hsu, S.3    Chien, C.4    Wu, J.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.