Constitutive expression of recombinant proteins in the methylotrophic yeast Hansenula polymorpha using the PMAI promoter

Yeast

Volumn 16, Issue 13, 2000, Pages 1191-1203

  Cox, Helen ^a   Mead, David ^b   Sudbery, Peter ^a   Eland, R Mark ^b   Mannazzu, Ilaria ^c   Evans, Leslie ^b  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

^b Delta Biotechnology Ltd   (United Kingdom)

^c UNIVERSITÀ POLITECNICA DELLE MARCHE   (Italy)

Author keywords

Constitutive promoter; Glucose oxidase; Hansenula polymorpha; Human serum albumin; Plasma membrane H+ ATPase; PMA1; Protein expression; RHA; Saccharomyces cerevisiae

Indexed keywords

ADENOSINE TRIPHOSPHATASE; GLUCOSE OXIDASE; METHANOL OXIDASE; OXIDOREDUCTASE; RECOMBINANT PROTEIN; SERUM ALBUMIN; UNCLASSIFIED DRUG;

ARTICLE; CELL DENSITY; CONTROLLED STUDY; ENZYME SYNTHESIS; FERMENTATION; GENE EXPRESSION REGULATION; GENE EXPRESSION SYSTEM; GENETIC RECOMBINATION; HANSENULA POLYMORPHA; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; REPORTER GENE;

ALCOHOL OXIDOREDUCTASES; BLOTTING, WESTERN; DNA, FUNGAL; DNA, RECOMBINANT; GENE EXPRESSION REGULATION, FUNGAL; GLUCOSE OXIDASE; HUMAN; PICHIA; PLASMIDS; PROMOTER REGIONS (GENETICS); PROTON-TRANSLOCATING ATPASES; RECOMBINANT FUSION PROTEINS; SERUM ALBUMIN; SUPPORT, NON-U.S. GOV'T; TRANSFORMATION, GENETIC;

PICHIA ANGUSTA; SACCHAROMYCES CEREVISIAE;

EID: 0034734850     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0061(20000930)16:13<1191::AID-YEA589>3.0.CO;2-2     Document Type: Article

References (35)

1. Derepression and partial insensitivity to carbon catabolite repression of the methanol dissimulatory enzymes in Hansenula polymorpha
2. Methonol metabolism in yeasts: Regulation of the synthesis of catabolic enzymes
3. Highly-efficient electrotransformation of the yeast Hansenula polymorpha
4. Review methylotrophic yeasts as factories for the production of foreign proteins
5. Foreign gene-expression in Hansenula polymorphaa system for the synthesis of small functional peptides
6. Expression of the alphagalactosidase from Cyamopsis tetragonoloba (guar) by Hansenula polymorpha
7. Screening and mutagenesis of moulds for improved glucose oxidase production
8. Stable multicopy vectors for high level secretion of recombinant human serum albumin by Kluyveromyces yeasts
9. Application of yeasts in gene expression studies: A comparison of Saccharomyces cerevisiae, Hansenula polymorpha and Kluyveromyces lactis - A review
10. Methylotrophic yeast Hansenula polymorpha as production organism for recombinant pharmaceuticals
11. Recombinant Hansenula polymorpha as a biocatalyst - Coexpression of the spinach glycolate oxidase (go) and the Saccharomyces cerevisiae catalase-t (cttl) gene
12. Transformation of the methylotrophic yeast Hansenula polymorpha
13. The methylotrophic yeasts
14. The production of heterologous plasma proteins
15. Expression of the glucose-oxidase gene from Aspergillus niger in Hansenula polymorpha and its use as a reporter gene to isolate regulatory mutations
16. Production of recombinant proteins by methylotrophic yeasts
17. Simultaneous expression of the S-surface and L-surface antigens of hepatitis-B, and formation of mixed particles in the methylotrophic yeast, Hansenula polymorpha
18. Disruption of the Saccharomyces cerevisiae YAP3 gene reduces the proteolytic degradation of secreted recombinant human albumin
19. A non-barbital buffer for immuno-electrophoresis and zone electrophoresis in agarose gels
20. + ATPase of Saccharomyces cerevisiae
21. Foreign gene-expression in yeast - A review
22. Transport across yeast vacuolar and plasma membranes
23. A human gene responsible for Zellweger syndrome that affects peroxisome assembly
24. Saccharomyces cerevisiae strains that overexpress heterologous proteins
25. The secretion of human serum albumin from the yeast Saccharomyces cerevisiae using five different secretion leader sequences
26. Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris
27. The non-Saccharomyces yeasts
28. Peroxisome biogenesis in the yeast Hansenula polymorpha: A structural and functional analysis
29. Development of a strain of a Hansenula polymorpha for the efficient expression of guar α-galactosidase
30. Isolation of the Plchia pastoris glyceraldehyde-3-phosphate gene and regulation and use of its promoter
31. High-level secretion of hirudin by Hansenula polymorphaauthentic processing of three different preprohirudins
32. Selection of promoter fragments involved in peroxisomal biosynthesis and metabolic pathway using glucose oxidase as a marker
33. Expression of the major bean proteins from theobroma-cacao (cocoa)in the yeasts Hansenula polymorpha and Saccharomyces cerevisiae
34. Production of two aprotinin variants in Hansenula polymorpha