Application of a wide-range yeast vector (CoMed™) system to recombinant protein production in dimorphic Arxula adeninivorans, methylotrophic Hansenula polymorpha and other yeasts

Microbial Cell Factories

Volumn 5, Issue , 2006, Pages

  Steinborn, Gerhard ^a   Böer, Erik ^a   Scholz, Anja ^a   Tag, Kristina ^a   Kunze, Gotthard ^a   Gellissen, Gerd ^b  

^a LEIBNIZ INSTITUTE OF PLANT GENETICS AND CROP PLANT RESEARCH IPK   (Germany)

^b PharmedArtis GmbH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; INTERLEUKIN 6; RECOMBINANT PROTEIN; RIBOSOME DNA;

ARTICLE; ARXULA ADENINIVORANS; AUXOTROPHY; CONTROLLED STUDY; CYTOKINE RELEASE; DNA SEQUENCE; ESCHERICHIA COLI; EXPRESSION VECTOR; FERMENTATION; GENE EXPRESSION; GENE SEQUENCE; GENE TARGETING; GENETIC MANIPULATION; GENETIC SELECTION; GENOMICS; HANSENULA POLYMORPHA; HOST; NONHUMAN; PLASMID; PROMOTER REGION; PROTEIN MODIFICATION; PROTEIN SECRETION; PROTEIN SYNTHESIS; YEAST;

ARXULA ADENINIVORANS; EUKARYOTA; PICHIA ANGUSTA;

EID: 33751323706     PISSN: 14752859     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-5-33     Document Type: Article

References (71)

1. Gellissen G: Production of recombinant proteins - Novel microbial and eukaryotic expression systems Weinheim: Wiley-VCH; 2005.
2. Gellissen G, Strasser AWM, Suckow M: Key and criteria to the selection of an expression platform. In Production of recombinant proteins - Novel microbial and eukaryotic expression systems Edited by: Gellissen G. Weinheim:Wiley-VCH; 2005:1-5.
3. Melmer G: Biopharmaceuticals and the industrial environment. In Production of recombinant proteins - Novel microbial and eukaryotic expression systems Edited by: Gellissen G. Weinheim: Wiley-VCH; 2005:361-383.
4. Harford N, Cabezon T, Colau B, Delisse A-M, Rutgers T, de Wilde M: Construction and characterization of a Saccharomyces cerevisiae strain (RIT4376) expressing hepatitis B surface antigen. Postgrad Med J 1987, 63:65-70.
5. Gellissen G: Heterologous protein production in methylotrophic yeasts. Appl Microbiol Biotechnol 2000, 54:741-750.
6. Kang HA, Gellissen G: Hansenula polymorpha. In Production of recombinant proteins - Novel microbial and eukaryotic expression systems Edited by: Gellissen G. Weinheim: Wiley-VCH; 2005:111-142.
7. Ilgen C, Lin-Cereghino J, Cregg JM: Pichia pastoris. In Production of recombinant proteins - Novel microbial and eukaryotic expression systems Edited by: Gellissen G. Weinheim: Wiley-VCH; 2005:143-162.
8. Terentiev Y, Gellissen G, Kunze G: Arxula adeninivorans - a non-conventional dimorphic yeast of great biotechnological potential. Recent Res Devel Appl Microbiol Biotechnol 2003, 1:135-145.
9. Böer E, Gellissen G, Kunze G: Arxula adeninivorans. In Production of recombinant proteins - Novel microbial and eukaryotic expression systems Edited by: Gellissen G. Weinheim: Wiley-VCH; 2005:89-110.
10. Hamilton SR, Bobrowicz P, Bobrowicz B, Davidson RC, Li H, Mitchell T, Nett JH, Rausch S, Stadheim TA, Wischnewski H, Wildt S, Gerngross TU: Production of complex human glycoproteins in yeast. Science 2003, 301:1244-1246.
11. Kim MW, Kim EJ, Kim J-Y, Park J-S, Oh D-B, Shimma J-I, Chiba Y, Jigami Y, Rhee SK, Kang HA: Functional characterization of the Hansenula polymorpha HOCI, OCHI, and OCRI genes as members of the yeast OCHI mannosyltransferase family involved in protein glycosylation. J Biol Chem 2006, 281:6261-6272.
12. Ramezani-Rad M, Hollenberg CP, Lauber J, Wedler H, Griess E, Wagner C, Albermann K, Hani J, Piontek M, Dahlems U, Gellissen G: The Hansenula polymorpha (strain CBS4732) genome sequencing and analysis. FEMS Yeast Res 2003, 4:207-215.
13. Oh KS, Kwon O, Oh YW, Sohn MJ, Jung S, Kim YK, Kim MG, Rhee SK, Gellissen G, Kang HA: Fabrication of a partial genome microarray of the methylotrophic yeast Hansenula polymorpha : optimization and evaluation for transcript profiling. J Microbiol Biotechnol 2004, 14:1239-1248.
14. Gellissen G, Kunze G, Gaillardin C, Cregg JM, Berardi E, Veenhuis M, van der Klei IJ: New yeast expression platforms based on methylotrophic Hansenula polymorpha and Pichia pastoris and on dimorphic Arxula adeninivorans and Yarrowia lipolytica - a comparison. FEMS Yeast Res 2005, 5:1079-1096.
15. Madzak C, Nicaud J-M, Gaillardin C: Yarrowia lipolytica. In Production of recombinant proteins - Novel microbial and eukaryotic expression systems Edited by: Gellissen G. Weinheim: Wiley-VCH; 2005:163-189.
16. Klabunde J, Kunze G, Gellissen G, Hollenberg CP: Integration of heterologous genes in several yeast species using vectors containing a Hansenula polymorpha-derived rDNA targeting element. FEMS Yeast Res 2003, 4:185-193.
17. Terentiev Y, Pico AH, Böer E, Wartmann T, Klabunde J, Breuer U, Babel W, Suckow M, Gellissen G, Kunze G: A wide-range integrative yeast expression system based on Arxula adeninivoransderived elements. J Ind Microbiol Biotechnol 2004, 31:223-228.
18. Klabunde J, Kunze G, Gellissen G, Hollenberg CP: Wide-range integrative expression vectors for fungi, based on ribosomal DNA elements. In Production of recombinant proteins - Novel microbial and eukaryotic expression systems Edited by: Gellissen G. Weinheim: Wiley-VCH; 2005:273-286.
19. Steinborn G, Gellissen G, Kunze G: Assessment of Hansenula polymorpha and Arxula adeninivorans-derived rDNA-targeting elements for the design of Arxula adeninivorans expression vectors. FEMS Yeast Res 2005, 5:1047-1054.
20. Wartmann T, Stoltenburg R, Böer E, Sieber H, Bartelsen O, Gellissen G, Kunze G: The ALEU2 gene - a new component for an Arxula adeninivorans-based expression platform. FEMS Yeast Res 2003, 3:223-232.
21. Wartmann T, Bellebna, C, Böer E, Bartelsen O, Gellissen G, Kunze G: The constitutive AHSB4 promoter - a novel component of the Arxula adeninivorans-based expression platform. Appl Microbiol Biotechnol 2003, 62:528-535.
22. Beggs JP, Guerineau M, Atkins JF: A map of the restriction targets in yeast 2 micron plasmid DNA cloned on bacteriophage lambda. Mol Gen Genet 1976, 17:287-294.
23. Gullov K, Friis J: Maintenance and copy number control of ARSI plasmids in Saccharomyces cerevisiae. Evidence of a mating type effect. Curr Genet 1985, 10:21-27.
24. Piontek M, Hagedorn J, Hollenberg CP, Gellissen G, Strasser AWM: Two novel gene expression systems based on the yeasts Schwanniomyces occidentolis and Pichia stipitis. Appl Microbiol Biotechnol 1998, 50:331-338.
25. Rösel H, Kunze G: Integrative transformation of the dimorphic yeast Arxula adeninivorans based on hygromycin B resistance. Curr Genet 1998, 33:157-163.
26. Rose M, Grisafi P, Botstein D: Structure and function of the yeast URA3 gene: expression in Escherichia coli. Gene 1984, 29:113-124.
27. Froman BE, Tait RC, Rodriguez RL: Nucleotide sequence of the 3′ terminal region of the LEU2 gene from Saccharomyces cerevisiae. Gene 1984, 31:257-261.
28. Thill GP, Davis GR, Stillman C, Holtz G, Brierley R, Engel M, Buckholtz R, Kenney J, Provow S, Vedvick T, Siegel RS: Positive and negative effects of multicopy integrated expression vectors on protein expression in Pichia pastoris. In Proceedings of the 6th International Syposium on the genetics of microorganisms Edited by: Heslot H, Davies J, Florent J, Bobichon L, Durand G, Penasse L. Paris: Société Francaise de Microbiologie; 1990:477-490.
29. Steinborn G, Wartmann T, Gellissen G, Kunze G: Construction of an Arxula adeninivorans host-vector system based on trp1 complementation. J Biotechnol 2006 in press.
30. Amuel C, Gellissen G, Hollenberg CP, Suckow M: Analysis of heatshock promoters in Hansenula polymorpha : TPSI, a novel element for heterologous gene expression. Biotechnol Bioprocess Eng 2000, 5:247-252.
31. Raschke WC, Neiditsch BR, Hendricks M, Cregg JM: Inducible expression of a heterologous protein in Hansenula polymorpha using the alcohol oxidase 1 promoter of Pichia pastoris. Gene 1996, 177:163-187.
32. Wartmann T, Böer E, Huarto Pico A, Sieber H, Bartelsen O, Gellissen G, Kunze G: High-level production of recombinant proteins by the dimorphic yeast Arxula adeninivorans. FEMS Yeast Res 2002, 2:363-369.
33. Bui DM, Kunze I, Förster S, Wartmann T, Horstmann C, Manteuffel R, Kunze G: Cloning and expression of an Arxula odeninivorans glucoamylase gene in Saccharomyces cerevisiae. Appl Microbiol Biotechnol 1996, 44:610-619.
34. Böer E, Mock HP, Bode R, Gellissen G, Kunze G: Anextracellular lipase from the dimorphic yeast Arxula adeninivorans. Molecular cloning of th ALIPI gene and characterization of the purified recombinant enzyme. Yeast 2005, 22:523-535.
35. Böer E, Wartmann T, Luther B, Manteuffel R, Bode R, Gellissen G, Kunze G: Characterization of the AINV gene and the encoded invertase from the dimorphic yeast. Arxula adeninivorans. Antonie van Leeuwenhoek 2004, 86:121-134.
36. Böer E, Wartmann T, Schmidt S, Bode R, Gellissen G, Kunze G: Characterization of the AXDH gene and the encoded xylitol dehydrogenase from the dimorphic yeast Arxula adeninivorans. Antonie van Leeuwenhoek 2004, 87:233-243.
37. Müller S, Sandal T, Kamp-Hansen P, Dalboge H: Comparison of expression systems in the yeasts Saccharomyces cerevisiae, Hansenula polymorpha, Kluyveromyceslactis, Schizosoccharomyces pombe and Yarrowialipolytica. Cloning of two novel promoters from Yarrowia lipolytica. Yeast 1998, 14:1267-1283.
38. Tonouchi N, Oouchi N, Kashima N, Kawai M, Nagase K, Okano A, Matsui H, Yamada K, Hirano T, Kishimoto T: High-level expression of BSF2/IL6 cDNA in Escherichia coli using a new type of expression/preparation system. J Biochem 1988, 104:30-34.
39. Guisez Y, Tison B, Vanderkerckhove J, Demolder J, Bauw G, Haegemann G, Fiers W, Contreras R: Production and purification of recombinant interleukin-6 secreted by the yeast Saccharomyces cerevisiae. Eur J Biochem 1991, 198:217-222.
40. Degelmann A, Müller F, Sieber H, Jenzelewski V, Suckow M, Strasser AWM, Gellissen G: Strain and process development for the production of human cytokines in Hansenula polymorpha. FEMS Yeast Res 2002, 2:349-361.
41. Gellissen G, Müller F, Sieber H, Tieke A, Jenzelewski V, Degelmann A, Strasser AWM: Production of cytokines in Hansenula polymorpha. In Hansenula polymorpha - biology and applications Edited by: Gellissen G. Weinheim: Wiley-VCH; 2002:229-254.
42. Proudfoot AE, Brown SC, Bernard AR, Bonnefoy JY, Kawashima EH: Recombinant human IL-6 expressed in E. coli undergoes selective N-terminal degradation: evidence that the protein consists of a stable core and a non-essential N-terminal. J Protein Chem 1993, 12:489-497.
43. Klabunde J, Diesel A, Waschk D, Gellissen G, Hollenberg CP, Suckow M: Single-step co-integration of multiple expressible heterologous genes into the ribosomal DNA of the methylotrophic yeast Hansenula polymorpha . Appl Microbiol Biotechnol 2002, 58:797-805.
44. Terentiev Y, Breuer U, Babel W, Kunze G: Non-conventional yeasts as producers of polyhydroxylalkanoates: genetic engineering of Arxula adeninivorans . Appl Microbiol Biotechnol 2004, 64:376-381.
45. Hahn T, Tag K, Riedel K. Uhlig S, Baronian K, Gellissen G, Kunze G: A novel estrogen sensor based on recombinant Arxula adeninivorans cells. Biosensors Bioelectronics 2006, 21:2078-2085.
46. Key LL, Ries WL, Rodriguiz RMI, Hatcher HC: Recombinant interferon-gamma therapy for osteopetrosis. J Pediatr 1992, 121:119-124.
47. Key LL, Rodriguiz RM, Willi SM, Wright NM, Hatcher HC, Eyre DR, Cure JK, Griffin PP, Ries WL: Long-term treatment of osteopetrosis with human interferon-gamma. N Engl J Med 1995, 332:1594-1599.
48. Arakawa T, Hsu YR, Chang D, Stebbing N. Altrock B: Structure and activity of human interferon-gamma. J Interferon Res 1986, 6:687-695.
49. Saraneva T, Pirhonen J, Cantell K, Julkonen I: N-glycosylation of interferon-gamma: glycans at Asn25 are critical for protease resistance. Biochem J 1995, 308:9-14.
50. Simons G, Remaut E, Allet B, Devos R, Fiers W: High-level expression of human interferon-gamma in Escherichia coli under control of the pL promoter of bacteriophage lambda. Gene 1984, 28:55-64.
51. Nishi T, Fujita T, Nishi-Takaoka C, Saito A, Matsumoto T, Sato M, Oka T, Itoh S, Yip YK, Vilcek J: Cloning and expression of a novel variant of human interferon-gamma cDNA. J Biochem 1985, 97:153-159.
52. Riske FJ, Cullen BR, Chizzonite R: Characterization of human interferon-gamma and human interleukin-2 from recombinant mammalian cell lines and peripheral blood lymphozytes. Lymphokine Cytokine Res 1991, 10:213-218.
53. Harmsen MM, Bruyne MI, Raue HA, Maat J: Oveeexpression of binding protein and disruption of the PMR1 gene synergistically stimulate secretion of bovine prochymosin but not plant thaumatin in yeast. Appl Microbiol Biotechnol 1996, 46:365-370.
54. Bao WG, Fukuhara H: Secretion of human proteins from yeast: stimulation by duplication of polyubiquitin and protein disulfideisomerase genes in Kluyveromyces lactis. Gene 2001, 272:103-110.
55. Ruohonen L, Toikkanen J, Tieaho V, Outola M, Soderlund H, Keranen S: Enhancement of protein secretion in Saccharomyces cerevisiae by overproduction of SSO protein, a late-acting component of the secretory machinery. Yeast 1997, 13:337-351.
56. Farwick M: Untersuchungen der Produktausbeute bei der Sekretion heterologer Proteine durch S. cerevisiae . In PhD thesis HHU Düsseldorf, 1998.
57. Müller F, Tieke A, Waschk D, Mühle C, Müller F, Seigelchifer M, Pesce A, Jenzelewski V, Gellissen G: Production of IFNα-2a in Hansenula polymorpha. Process Biochem 2002, 38:15-25.
58. Klabunde J: Koproduktion pharmazeutischer Proteine und Hilfsfaktoren zur Optimierung mikrobieller Expressionssysteme bei Beschränkung auf ein einziges integratives Vektorsystem. In PhD thesis HHU Düsseldorf; 2003.
59. Kunze G, Kunze I: Characterization of Arxula adeninivorans strains from different habitats. Antonie van Leeuwenhoek 1994, 65:29-34.
60. Samsonova IA, Kunze G, Bode R, Böttcher F: A set of genetic markers for the chromosomes of the imperfect yeast Arxula adeninivorans. Yeast 1996, 12:1209-1217.
61. Heinemeyer W, Kleinschmidt JA, Saidowsky J, Escher C, Wolf DH: Proteinase yscE, the yeast proteasome/multicatalytic-multifunctional proteinase: mutants unravel its function in stress induced proteolysis and uncover its necessity for cell survival. EMBO J 1991, 10:555-562.
62. Rose MD, Winston F, Hieter P: Methods in Yeast Genetics. A Laboratory Manual Cold Spring Harbor New York: Cold Spring Harbor Laboratory Press; 1990.
63. 6 by Candida albicans in hydrocarbon medium. J Ferment Technol 1967, 45:617-623.
64. Dohmen RJ, Strasser AW, Höner CB, Hollenberg CP: An efficient transformation procedure enabling long-term storage of competent cells of various yeast genera. Yeast 1991, 7:691-692.
65. Wartmann T, Erdmann J, Kunze I, Kunze G: Morphology-related effects on gene expression and protein accumulation of the yeast Arxula adeninivorans LS3. Arch Microbiol 2000, 173:253-261.
66. Kunze I, Hensel G, Adler K, Bernard J, Neubohn B, Nilsson C, Stoltenburg R, Kohlwein SD, Kunze G: The green fluorescent protein targets secretary proteins to the yeast vacuole. Biochim Biophys Acta 1999, 1410:287-298.
67. Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976, 72:248-254.
68. Riis V, Mai W: Gas chromatographic determination of poly-β-hydroxybutyric acid in microbial biomass after hydrochloric acid propanolysis. J Chromatogr 1988, 445:285-289.
69. Breuer U, Ackermann JU, Babel W: Accumulation of poly(3-hydroxybutyric acid) and overproduction of exopolysaccharides in a mutant of a methylotrophic bacterium. Can J Microbiol 1995, 41 (Suppl 1):55-59.
70. Büttner R, Bode R, Birnbaum D: Characterization of extracellular acid phosphatases from the yeast Arxula adeninivorans. Zentralbl Mikrobiol 1991, 146:399-406.
71. Sajidan A, Farouk A, Greiner R, Jungblut P, Müller EC, Borriss R: Molecular and physiological characterisation of a 3-phytase from soil bacterium Klebsiello sp. ASR1. Appl Microbiol Biotechnol 2004, 65:110-118.