Sensitive multiplexed analysis of kinase activities and activity-based kinase identification

Nature Biotechnology

Volumn 27, Issue 10, 2009, Pages 933-940

  Kubota, Kazuishi ^a,e,f   Anjum, Rana ^a,e,f   Yu, Yonghao ^a   Kunz, Ryan C ^a   Andersen, Jannik N ^b   Kraus, Manfred ^b   Keilhack, Heike ^b   Nagashima, Kumiko ^b   Krauss, Stefan ^b   Paweletz, Cloud ^b   Hendrickson, Ronald C ^b   Feldman, Adam S ^c   Wu, Chin Lee ^c   Rush, John ^d   Villén, Judit ^a   Gygi, Steven P ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b MERCK RESEARCH LABORATORIES   (United States)

^c MASSACHUSETTS GENERAL HOSPITAL   (United States)

^d CELL SIGNALING TECHNOLOGY   (United States)

^e DAIICHI SANKYO CO   (Japan)

^f ARIAD PHARMACEUTICALS INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVITY-BASED; CANCER CELL LINES; CELL LYSATES; COMPETITION EFFECTS; GENETIC BACKGROUNDS; HIER-ARCHICAL CLUSTERING; KINASE ACTIVITY; MITOGENS; MULTIPLEXED ANALYSIS; NANOGRAMS; PHARMACOLOGICAL INHIBITORS; PROTEIN COMPLEXES; REACTION STRATEGIES; SIGNALING PATHWAYS; SYNTHETIC PEPTIDE;

AMINES; CELL CULTURE; CELL MEMBRANES; MASS SPECTROMETERS; MASS SPECTROMETRY; PEPTIDES; PHOSPHORYLATION; WELL STIMULATION;

SIGNALING;

PHOSPHOTRANSFERASE;

ANIMAL CELL; ARTICLE; CANCER CELL CULTURE; CELL CYCLE; CELL LINE; CELL LYSATE; COMPLEX FORMATION; ENZYME ACTIVITY; ENZYME PHOSPHORYLATION; HUMAN; HUMAN CELL; MASS SPECTROMETRY; NONHUMAN; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PHOSPHORYLATION; QUANTITATIVE ANALYSIS; SIGNAL TRANSDUCTION;

CELL CYCLE; CELLS, CULTURED; CLUSTER ANALYSIS; COMPUTATIONAL BIOLOGY; CYCLIN B; EPIDERMAL GROWTH FACTOR; HELA CELLS; HUMANS; INSULIN; MASS SPECTROMETRY; PHOSPHORYLATION; PROTEIN KINASES; PROTO-ONCOGENE PROTEINS C-AKT; RECEPTOR PROTEIN-TYROSINE KINASES; REPRODUCIBILITY OF RESULTS; SIGNAL TRANSDUCTION; TETRADECANOYLPHORBOL ACETATE;

EID: 70349961430     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt.1566     Document Type: Article

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