Effect of radiofrequency electromagnetic field exposure on in vitro models of neurodegenerative disease

Bioelectromagnetics

Volumn 30, Issue 7, 2009, Pages 564-572

  Del Vecchio, Giovanna ^a   Giuliani, Alessandro ^a   Fernandez, Mercedes ^a   Mesirca, Pietro ^a   Bersani, Ferdinando ^a   Pinto, Rosanna ^b   Ardoino, Lucia ^b   Lovisolo, Giorgio A ^b   Giardino, Luciana ^a   Calzà, Laura ^a  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b ENEA C R CASACCIA   (Italy)

Author keywords

Neurodegeneration; Neuronal toxicity; Radiofrequency EMF

Indexed keywords

CELL CULTURE; CELLS; ELECTROMAGNETIC FIELDS; GLYCOPROTEINS; HYDROGEN PEROXIDE; NEURODEGENERATIVE DISEASES; NEURONS;

CORTICAL NEURONS; ELECTROMAGNETIC FIELD EXPOSURE; GLOBAL SYSTEM FOR MOBILES; MOBILE TELECOMMUNICATIONS; NEURAL CELLS; NEURODEGENERATION; NEURONAL TOXICITY; RADIOFREQUENCIES; RADIOFREQUENCY ELECTROMAGNETIC FIELD; RADIOFREQUENCY EMF;

OXIDATION;

AMYLOID BETA PROTEIN[25-35]; GLUTAMIC ACID; HYDROGEN PEROXIDE;

ANIMAL CELL; ARTICLE; BRAIN CELL; CELL PROLIFERATION; CELL VIABILITY; CONTROLLED STUDY; DEGENERATIVE DISEASE; ELECTROMAGNETIC FIELD; NERVE CELL; NEUROTOXICITY; NONHUMAN; PRIORITY JOURNAL; RADIATION ABSORPTION; RADIOFREQUENCY RADIATION; RAT; TELECOMMUNICATION; WIRELESS COMMUNICATION;

RATTUS;

ANIMALS; CELL SURVIVAL; CELLS, CULTURED; ENVIRONMENTAL EXPOSURE; GLUTAMIC ACID; MICROWAVES; NEURODEGENERATIVE DISEASES; NEURONS; RATS;

EID: 70249149656     PISSN: 01978462     EISSN: 1521186X     Source Type: Journal    
DOI: 10.1002/bem.20507     Document Type: Article

References (54)

1. Alberts B, Bray D, Lewis J, Raff M, Roberts K, Watson JD. 1994. Molecular biology of the cell. 3rd edition. New York and London: Garland. pp. 1173-1175.
2. Ardoino L, Lopresto V, Mancini S, Marino C, Pinto R, Lovisolo GA, 2005. A radio-frequency system for in vivo pilot experiments aimed at the studies on biological effects of electromagnetic fields. Phys Med Biol 50:3643-3654. (Pubitemid 41019102)
3. Arundine M, Tymiansky M. 2003. Molecular mechanisms of calcium-dependent neurodegeneration in excitotoxicity. Cell Calcium 34:325-337. (Pubitemid 37021228)
4. Berg G, Spalleck J, Schuz J, Schlehofer B, Bohler E, Schlaefer K, Hettinger I, Kunna-Grass K, Wahrendorf J, Blettner M. 2006. Occupational exposure to radio frequency/microwave radiation and the risk of brain tumors: Interphone Study Group, Germany. Am J Epidemiol 164:538-548. (Pubitemid 44412782)
5. Capri M, Scarcella E, Fumelli C, Bianchi E, Salvioli S, Mesirca P, Agostini C, Antolini A, Schiavoni A, Castellani G, Bersani F, Franceschi C. 2004. In vitro exposure of human lymphocytes to 900 MHz CW and GSM modulated radiofrequency: Studies of proliferation, apoptosis and mitochondrial membrane potential. Radiat Res 162:211-218.
6. Chang DT, Reynolds IJ. 2006. Differences in mitochondrial movement and morphology in young and mature primary cortical neurons in culture. Neuroscience 141:727-736.
7. Crawford ML. 1974. Generation of standard EM fields using TEM transmission cells. IEEE Trans Electromagn Compat 16:189-195.
8. Czyz J, Guan K, Zeng Q, Nikolova T, Meister A, Schonborn F, Schuderer J, Kuster N, Wobus AM. 2004. High frequency electromagnetic fields (GSM signals) affect gene expression levels in tumor suppressor p53-deficient embryonic stem cells. Bioelectromagnetics 25:296-307. (Pubitemid 38596476)
9. Dickstein DL, Kabaso D, Rocher AB, Luebke JI, Wearne SL, Hof PR. 2007. Changes in the structural complexity of the aged brain. Aging Cell 6:275-284. (Pubitemid 46785133)
10. Fass U, Panickar K, Personett D, Bryan D, Williams K, Gonzales J, Sugaya K, McKinney M. 2000. Differential vulnerability of primary cultured cholinergic neurons to nitric oxide excess. NeuroReport 11:931-936. (Pubitemid 30235431)
11. Fernandez M, Pirondi S, Antonelli T, Ferraro L, Giardino L, Calza L. 2005. Role of c-Fos protein on glutamate toxicity in primary neural hippocampal cells. J Neurosci Res 82:115-125. (Pubitemid 41604803)
12. Franzellitti S, Valbonesi P, Contin A, Biondi C, Fabbri E. 2008. HSP70 expression in human trophoblast cells exposed to different 1.8 Ghz mobile phone signals. Radiat Res 170:488-497.
13. Friedman J, Kraus S, Hauptman Y, Schiff Y, Seger R. 2007. Mechanism of a short-term ERK activation by electromagnetic fields at mobile phone frequency. Biochem J 405:559-568. (Pubitemid 47172054)
14. Guy AW, Chou CK, McDougall JA. 1999. A quarter century of in vitro research: A new look at exposure methods. Bioelectromagnetics 20:21-39. (Pubitemid 129548504)
15. Hammond DN, Wainer BH, Tonsgard JH, Heller A. 1986. Neuronal properties of clonal hybrid cell lines derived from central cholinergic neurons. Science 234:1237-1240. (Pubitemid 17232362)
16. Hammond DN, Lee HJ, Tonsgard JH, Wainer BH. 1990. Development and characterization of clonal cell lines derived from septal cholinergic neurons. Brain Res 512:190-200.
17. Hara MR, Snyder SH. 2007. Cell signaling and neuronal death. Annu Rev Pharmacol Toxicol 47:117-141.
18. Hossmann KA, Hermann DM. 2003. Effects of electromagnetic radiation of mobile phones on the central nervous system. Bioelectromagnetics 24:49-62. (Pubitemid 38622842)
19. Höytö A, Luukkonen J, Juutilainen J, Naarala J. 2008. Proliferation, oxidative stress and cell death in cells exposed to 872 MHz radiofrequency radiation and oxidants. Radiat Res 170:235-243.
20. Huang TQ, Lee MS, Oh EH, Kalinec F, Zhang BT, Seo JS, Park WY. 2008. Characterization of biological effect of 1763 MHz radiofrequency exposure on auditory hair cells. Int J Radiat Biol 84:909-915. (Pubitemid 352766024)
21. Ilhan A, Gurel A, Armutcu F, Kamisli S, Iraz M, Akyol O, Ozen S. 2004. Ginkgo biloba prevents mobile phone-induced oxidative stress in rat brain. Clin Chim Acta 340:153-162. (Pubitemid 38167519)
22. Irmak MK, Fadillioglu E, Güleç M, Erdogan H, Yagmurca M, Akyol O. 2002. Effects of electromagnetic radiation from a cellular telephone on the oxidant and antioxidant levels in rabbits. Cell Biochem Funct 20:279-283. (Pubitemid 35340062)
23. Joubert V, Leveque P, Rametti A, Collin A, Bourthoumieu S, Yardin C. 2006. Microwave exposure of neuronal cells in vitro: Study of apoptosis. Int J Radiat Biol 82:267-275. (Pubitemid 43754925)
24. Joubert V, Leveque P, Cueille M, Bourthoumieu S, Yardin C. 2007. No apoptosis is induced in rat cortical neurons exposed to GSM phone fields. Bioelectromagnetics 28:115-121. (Pubitemid 46190946)
25. Kan P, Simonsen SE, Lyon JL, Kestle JR. 2008. Cellular phone use and brain tumor: A meta-analysis. J Neurooncol 86:71-78. (Pubitemid 350263435)
26. Kaprana AE, Karatzanis AD, Prokopakis EP, Panagiotaki IE, Vardiambasis IO, Adamidis G, Christodoulou P, Velegrakis GA. 2008. Studying the effects of mobile phone use on the auditory system and the central nervous system: A review of the literature and future directions. Eur Arch Otorhinolaryngol 265:1011-1019.
27. Kuriyama K, Okhuma S. 1990. Cell culture. In: Conn PM, editor. Methods in neuroscience Vol. 2: San Diego: Academic Press. pp. 103-116.
28. Lantow M, Lupke M, Frahm J, Mattson MO, Kuster N, Simkó M. 2006. ROS release and Hsp70 expression after exposure to 1.800 MHz radiofrequency electromagnetic fields in primary human monocytes and lymphocytes. Radiat Environ Biophys 45:55-62. (Pubitemid 44165445)
29. Lee HJ, Hammond DN, Large TH, Wainer BH. 1990. Immortalized young adult neurons from the septal region: Generation and characterization. Brain Res Dev Brain Res 52:219-228.
30. Leszczynski D, Joenvaara S, Reivinen J, Kuokka R. 2002. Non thermal activation of the hsp27/p38MAPK stress pathway by mobile phone radiation in human endothelial cells: Molecular mechanism for cancer and blood-brain barrier-related effects. Differentiation 70:120-129. (Pubitemid 38230562)
31. Lipton SA, Rosenberg PA. 1994. Excitatory amino acids as a final common pathway for neurologic disorders. N Engl J Med 330:613-622. (Pubitemid 24984686)
32. Luukkonen J, Hakulinen P, Mäki-Paakkanen J, Juutilainen J, Naarala J. 2009. Enhancement of chemically induced reactive oxygen species production and DNA damage in human SH-SY5Y neuroblastoma cells by 872MHz radiofrequency radiation. Mutat Res 662:54-58.
33. Malik MA, Greenwood CE, Blusztajin JK, Berse B. 2000. Cholinergic differentiation triggered by blocking cell proliferation and treatment with all-trans retinoic acid. Brain Res 874:178-185. (Pubitemid 30674242)
34. Mausset-Bonnefont AL, Hirbec H, Bonnefont X, Privat A, Vignon J, de Sèze R. 2004. Acute exposure to GSM 900-MHz electromagnetic fields induces glial reactivity and biochemical modifications in the rat brain. Neurobiol Dis 17:445-454. (Pubitemid 39574878)
35. Meral I, Mert H, Mert N, Deger Y, Yoruk I, Yetkin A, Keskin S. 2007. Effects of 900-MHz electromagnetic field emitted from cellular phone on brain oxidative stress and some vitamin levels of guinea pigs. Brain Res 1169:120-124. (Pubitemid 47374099)
36. Merola P, Marino C, Lovisolo GA, Pinto R, Laconi C, Negroni A. 2006. Proliferation and apoptosis in a neuroblastoma cell line exposed to 900 MHz modulated radiofrequency field. Bioelectromagnetics 27:164-171.
37. Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 65:55-63. (Pubitemid 14203433)
38. Moustafa YM, Moustafa RM, Belacy A, Abou-El-Ela SH, Ali FM. 2001. Effects of acute exposure to the radiofrequency fields of cellular phones on plasma lipid peroxide and antioxidase activities in human erythrocytes. J Pharm Biomed Anal 26:605-608. (Pubitemid 32763230)
39. Newman M, Musgrave FI, Lardelli M. 2007. Alzheimer disease: Amyloidogenesis, the presenilins and animal models. Biochim Biophys Acta 1772:285-297. (Pubitemid 46275437)
40. Nikoloski N, Fröhlich J, Samaras T, Schuderer J, Kuster N. 2005. Re-evaluation and improved design of the TEM cell invitro exposure unit for replication studies. Bioelectromagnetics 26:215-224. (Pubitemid 40463427)
41. Nikolova T, Czyz J, Rolletschek A, Blyszczuk P, Fuchs J, Jovchtev G, Schuderer J, Kuster N, Wobus AM. 2005. Electromagnetic fields affect transcript levels of apoptosis-related genes in embryonic stem cell-derived neural progenitor cells. FASEB J 19:1686-1688. (Pubitemid 41429530)
42. Pedersen WA, Kloczewiak MA, Blusztajn JK. 1996. Amyloid beta-protein reduces acetylcholine synthesis in a cell line derived from cholinergic neurons of the basal forebrain. Proc Natl Acad Sci USA 93:8068-8071.
43. Personett D, Fass U, Panickar K, McKinney M. 2000. Retinoic acid-mediated enhancement of the cholinergic/neuronal nitric oxide synthase phenotype of the medial septal SN56 clone: Establishment of a nitric oxide-sensitive proapoptotic state. J Neurochem 74:2412-2424. (Pubitemid 30314217)
44. Pike CJ, Walencewicz AJ, Glabe CG, Cotman CW. 1991. In vitro aging of beta-amyloid protein causes peptide aggregation and neurotoxicity. Brain Res 563:311-314.
45. Pirondi S, Fernandez M, Schmidt R, Hokfelt T, Giardino L, Calza L. 2005. The galanin-R2 agonist AR-M1896 reduces glutamate toxicity in primary neural hippocampal cells. J Neurochem 95:821-833. (Pubitemid 41567173)
46. Port M, Abend M, Romer B, Van Beuningen D. 2003. Influence of high-frequency electromagnetic fields on different modes of cell death and gene expression. Int J Radiat Biol 79:701-708. (Pubitemid 37378537)
47. Repacholi MH. 2001. Health risks from the use of mobile phones. Toxicol Lett 120:323-331. (Pubitemid 32375099)
48. Stagg RB, Thomas WJ, Jones RA, Adey WR. 1997. DNA synthesis and cell proliferation in C6 glioma and primary glial cells exposed to 836.55 MHz modulated radiofrequency field. Bioelectromagnetics 18:230-236.
49. Suzaki Y, Yoshizumi M, Kagami S, Koyama AH, Taketani Y, Houchi H, Tsuchiya K, Takeda E, Tamaki T. 2002. Hydrogen peroxide stimulates c-Src-mediated big mitogen-activated protein kinase 1 (BMK1) and the MEF2C signaling pathway in PC12 cells: Potential role in cell survival following oxidative insults. J Biol Chem 277:9614-9621. (Pubitemid 34953051)
50. Valentini E, Curcio G, Moroni F, Ferrara M, De Gennaro L, Bertini M. 2007. Neurophysiological effects of mobile phone electromagnetic fields on humans: A comprehensive review. Bioelectromagnetics 28:415-432. (Pubitemid 350194132)
51. Whittemore ER, Loo DT, Watt JA, Cotmans CW. 1995. A detailed analysis of hydrogen peroxide-induced cell death in primary neuronal culture. Neuroscience 67:921-932.
52. Xu S, Ning W, Xu Z, Zhou S, Chiang H, Luo J. 2006. Chronic exposure to GSM 1800-MHz microwaves reduces excitatory synaptic activity in cultured hippocampal neurons. Neurosci Lett 398:253-257. (Pubitemid 43673707)
53. Yoshizumi M, Kogame T, Suzaki Y, Fujita Y, Kyaw M, Kirima K, Ishizawa K, Tsuchiya K, Kagami S, Tamaki T. 2002. Ebselen attenuates oxidative stress-induced apoptosis via the inhibition of the c-Jun N-terminal kinase and activator protein-1 signalling pathway in PC12 cells. Br J Pharmacol 136:1023-1032. (Pubitemid 34888379)
54. Zmyslony M, Politanski P, Rajkowska E, Szymczak W, Jajte J. 2004. Acute exposure to 930 MHz CW electromagnetic radiation in vitro affects reactive oxygen species level in rat lymphocytes treated by iron ions. Bioelectromagnetics 25:324-328. (Pubitemid 38890812)