Proliferation, oxidative stress and cell death in cells exposed to 872 MHz radiofrequency radiation and oxidants

Radiation Research

Volumn 170, Issue 2, 2008, Pages 235-243

  Höytö, Anne ^a   Luukkonen, Jukka ^a   Juutilainen, Jukka ^a   Naarala, Jonne ^a  

^a UNIVERSITY OF EASTERN FINLAND   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

CASPASE 3; DNA; GLUTATHIONE; MENADIONE; REACTIVE OXYGEN METABOLITE; TERT BUTYL HYDROPEROXIDE;

ANIMAL CELL; ARTICLE; CELL DEATH; CELL PROLIFERATION; CELL VIABILITY; CHEMICAL STRESS; CONTROLLED STUDY; DNA FRAGMENTATION; ENZYME ACTIVITY; FIBROBLAST; HUMAN; HUMAN CELL; LIPID PEROXIDATION; MOBILE PHONE; MOUSE; NEUROBLASTOMA; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; RADIATION EXPOSURE; RADIOFREQUENCY RADIATION; STATISTICAL SIGNIFICANCE;

ANIMALS; APOPTOSIS; CELL LINE; CELL PROLIFERATION; DOSE-RESPONSE RELATIONSHIP, DRUG; DOSE-RESPONSE RELATIONSHIP, RADIATION; ENVIRONMENTAL EXPOSURE; FIBROBLASTS; HUMANS; MICE; NEURONS; OXIDANTS; OXIDATIVE STRESS;

EID: 49249119352     PISSN: 00337587     EISSN: None     Source Type: Journal    
DOI: 10.1667/RR1322.1     Document Type: Article

References (52)

1. L. M. Penafiel, T. Litovitz, D. Krause, A. Desta and J. M. Mullins, Role of modulation on the effect of microwaves on ornithine decarboxylase activity in L929 cells. Bioelectromagnetics 18, 132-141 (1997).
2. M. Capri, E. Scarcella, C. Fumelli, E. Bianchi, S. Salvioli, P. Mesirca, C. Agostini, A. Antolini, A. Schiavoni and C. Franceschi, In vitro exposure of human lymphocytes to 900 MHz CW and GSM modulated radiofrequency: Studies of proliferation, apoptosis and mitochondrial membrane potential. Radiat. Res. 162, 211-218 (2004).
3. A. Markkanen, P. Penttinen, J. Naarala, J. Pelkonen, A. P. Sihvonen and J. Juutilainen, Apoptosis induced by ultraviolet radiation is enhanced by amplitude modulated radiofrequency radiation in mutant yeast cells. Bioelectromagnetics 25, 127-133 (2004).
4. J. Juutilainen and R. de Seze, Biological effects of amplitude-modulated radiofrequency radiation. Scand. J. Work Environ. Health 24, 245-254 (1998).
5. L. Heynick, S. Johnston and P. Mason, Radio frequency electromagnetic fields: cancer, mutagenesis, and genotoxicity. Bioelectromagnetics S6, S74-S100 (2003).
6. J. E. Moulder, K. R. Foster, L. S. Erdreich and J. P. McNamee, Mobile phones, mobile phone base stations and cancer: a review. Int. J. Radiat. Biol. 81, 189-203 (2005).
7. D. Krewski, C. V. Byus, B. W. Glickman, W. G. Lotz, R. Mandeville, M. L. McBride, F. S. Prato and D. F. Weaver, Recent advances in research on radiofrequency fields and health. J. Toxicol. Environ. Health B Crit. Rev. 4, 145-159 (2001).
8. D. Krewski, C. V. Byus, B. W. Glickman, W. G. Lotz, R. Mandeville, M. L. McBride, F. S. Prato and D. F. Weaver, Potential health risks of radiofrequency fields from wireless telecommunication devices. J. Toxicol. Environ. Health B Crit. Rev. 4, 1-143 (2001).
9. S. F. Cleary, L. M. Liu and R. E. Merchant, Glioma proliferation modulated in vitro by isothermal radiofrequency radiation exposure. Radiat. Res. 121, 38-45 (1990).
10. S. F. Cleary, L. M. Liu and R. E. Merchant, In vitro lymphocyte proliferation induced by radio-frequency electromagnetic radiation under isothermal conditions. Bioelectromagnetics 11, 47-56 (1990).
11. S. Velizarov, P. Raskmark and S. Kwee, The effects of radiofrequency fields on cell proliferation are non-thermal. Bioelectrochem. Bioenerg. 48, 177-180 (1999).
12. S. F. Cleary, Z. Du, G. Cao, L. M. Liu and C. McCrady, Effect of isothermal radiofrequency radiation on cytolytic T lymphocytes. FASEB J. 10, 913-919 (1996).
13. S. Kwee and P. Raskmark, Changes in cell proliferation due to environmental non-ionizing radiation 2. Microwave radiation. Bioelectrochem. Bioenerg. 44, 251-255 (1998).
14. D. Krause, J. M. Mullins, L. M. Penafiel, R. Meister and R. M. Nardone, Microwave exposure alters the expression of 2-5A-dependent RNase. Radiat. Res. 127, 164-170 (1991).
15. R. B. Stagg, W. J. Thomas, R. A. Jones and W. R. Adey, DNA synthesis and cell proliferation in C6 glioma and primary glial cells exposed to a 836.55 MHz modulated radiofrequency field. Bioelectromagnetics 18, 230-236 (1997).
16. Vijayalaxmi, N. Mohan, M. L. Meltz and M. A. Wittler, Proliferation and cytogenetic studies in human blood lymphocytes exposed in vitro to 2450 MHz radiofrequency radiation. Int. J. Radiat. Biol. 72, 751-757 (1997).
17. T. Nikolova, J. Czyz, A. Rolletschek, P. Blyszczuk, J. Fuchs, G. Jovtchev, J. Schuderer, N. Kuster and A. M. Wobus, Electromagnetic fields affect transcript levels of apoptosis-related genes in embryonic stem cell-derived neural progenitor cells. FASEB J. 19, 1686-1688 (2005).
18. S. Lixia, Y. Ke, W. Kaijun, L. Deqiang, H. Huajun, G. Xiangwei, W. Baohong, Z. Wei, L. Jianling and W. Wei, Effects of 1.8 GHz radiofrequency field on DNA damage and expression of heat shock protein 70 in human lens epithelial cells. Mutat. Res. 602, 135-142 (2006).
19. P. Merola, C. Marino, G. A. Lovisolo, R. Pinto, C. Laconi and A. Negroni, Proliferation and apoptosis in a neuroblastoma cell line exposed to 900 MHz modulated radiofrequency field. Bioelectromagnetics 27, 164-171 (2006).
20. S. Sanchez, A. Milochau, G. Ruffie, F. Poulletier de Gannes, I. Lagroye, E. Haro, J. E. Surleve-Bazeille, B. Billaudel, M. Lassegues and B. Veyret, Human skin cell stress response to GSM-900 mobile phone signals. In vitro study on isolated primary cells and reconstructed epidermis. FEBS J. 273, 5491-5507 (2006).
21. M. R. Scarfi, A. M. Fresegna, P. Villani, R. Pinto, C. Marino, M. Sarti, P. Altavista, A. Sannino and G. A. Lovisolo, Exposure to radiofrequency radiation (900 MHz, GSM signal) does not affect micronucleus frequency and cell proliferation in human peripheral blood lymphocytes: An interlaboratory study. Radiat. Res. 165, 655-663 (2006).
22. Y. Takashima, H. Hirose, S. Koyama, Y. Suzuki, M. Taki and J. Miyakoshi, Effects of continuous and intermittent exposure to RF fields with a wide range of SARs on cell growth, survival, and cell cycle distribution. Bioelectromagnetics 27, 392-400 (2006).
23. M. Port, M. Abend, B. Romer and D. Van Beuningen, Influence of high-frequency electromagnetic fields on different modes of cell death and gene expression. Int. J. Radiat. Biol. 79, 701-708 (2003).
24. M. Capri, E. Scarcella, E. Bianchi, C. Fumelli, P. Mesirca, C. Agostini, D. Remondini, J. Schuderer, N. Kuster and F. Bersani, 1800 Hz radiofrequency (mobile phones, different global system for mobile communication modulations) does not affect apoptosis and heat shock protein 70 level in peripheral blood mononuclear cells from young and old donors. Int. J. Radiat. Biol. 80, 389-397 (2004).
25. G. J. Hook, P. Zhang, I. Lagroye, L. Li, R. Higashikubo, E. G. Moros, W. L. Straube, W. F. Pickard, J. D. Baty and J. L. Roti Roti, Measurement of DNA damage and apoptosis in molt-4 cells after in vitro exposure to radiofrequency radiation. Radiat. Res. 161, 193-200 (2004).
26. I. Y. Belyaev, L. Hillert, M. Protopopova, C. Tamm, L. O. G. Malmgren, B. R. R. Persson, G. Selivanova and M. Harms-Ringdahl, 915 MHz microwaves and 50 Hz magnetic field affect chromatin conformation and 53 BP1 foci in human lymphocytes from hypersensitive and healthy persons. Bioelectromagnetics 26, 173-184 (2005).
27. E. Gurisik, K. Warton, D. K. Martin and S. M. Valenzuela, An in vitro study of the effects of exposure to a GSM signal in two human cell lines: Monocytic U937 and neuroblastoma SK-N-SH. Cell Biol. Int. 30, 793-799 (2006).
28. H. Hirose, N. Sakuma, N. Kaji, T. Suhara, M. Sekijima, T. Nojima and J. Miyakoshi, Phosphorylation and gene expression of p 53 are not affected in human cells exposed to 2.1425 GHz band CW or WCDMA modulated radiation allocated to mobile radio base stations. Bioelectromagnetics 27, 494-504 (2006).
29. V. Joubert, P. Leveque, A. Rametti, A. Collin, S. Bourthoumieu and C. Yardin, Microwave exposure of neuronal cells in vitro: Study of apoptosis. Int. J. Radiat. Biol. 82, 267-275 (2006).
30. M. Lantow, T. Viergutz, D. G. Weiss and M. Simko, Comparative study of cell cycle kinetics and induction of apoptosis or necrosis after exposure of human Mono Mac 6 cells to radiofrequency radiation. Radiat. Res. 166, 539-543 (2006).
31. V. Chauhan, A. Mariampillai, B. C. Kutzner, R. C. Wilkins, C. Ferrarotto, P. V. Bellier, L. Marro, G. B. Gajda, E. Lemay and J. P. McNamee, Evaluating the biological effects of intermittent 1.9 GHz pulse-modulated radiofrequency fields in a series of human-derived cell lines. Radiat. Res. 167, 87-93 (2007).
32. V. Joubert, P. Leveque, M. Cueille, S. Bourthoumieu and C. Yardin, No apoptosis is induced in rat cortical neurons exposed to GSM phone fields. Bioelectromagnetics 28, 115-121 (2007).
33. S. Sanchez, E. Haro, G. Ruffie, B. Veyret and I. Lagroye, In vitro study of the stress response of human skin cells to GSM-1800 mobile phone signals compared to UVB radiation and heat shock. Radiat. Res. 167, 572-580 (2007).
34. A. Peinnequin, A. Piriou, J. Mathieu, V. Dabouis, C. Sebbah, R. Malabiau and J. C. Debouzy, Non-thermal effects of continuous 2.45 GHz microwaves on fas-induced apoptosis in human Jurkat T-cell line. Bioelectrochemistry 51, 157-161 (2000).
35. K. Maeda, T. Maeda and Y. Qi, In vitro and in vivo induction of human LoVo cells into apoptotic process by non-invasive microwave treatment: A potentially novel approach for physical therapy of human colorectal cancer. Oncol. Rep. 11, 771-775 (2004).
36. F. Marinelli, D. La Sala, G. Cicciotti, L. Cattini, C. Trimarchi, S. Putti, A. Zamparelli, L. Giuliani, G. Tomassetti and C. Cinti, Exposure to 900 MHz electromagnetic field induces an unbalance between pro-apoptotic and pro-survival signals in T-lymphoblastoid leukemia CCRF-CEM cells. J. Cell. Physiol. 198, 324-332 (2004).
37. M. Caraglia, M. Marra, F. Mancinelli, G. D'Ambrosio, R. Massa, A. Giordano, A. Budillon, A. Abbruzzese and E. Bismuto, Electromagnetic fields at mobile phone frequency induce apoptosis and inactivation of the multi-chaperone complex in human epidermoid cancer cells. J. Cell. Physiol. 204, 539-548 (2005).
38. T. Y. Zhao, S. P. Zou and P. E. Knapp, Exposure to cell phone radiation up-regulates apoptosis genes in primary cultures of neurons and astrocytes. Neurosci. Lett. 412, 34-38 (2007).
39. J. Friedman, S. Kraus, Y. Hauptman, Y. Schiff and R. Seger, Mechanism of a short-term ERK activation by electromagnetic fields at mobile phone frequency. Biochem. J. 405, 559-568 (2007).
40. G. J. Hook, D. R. Spitz, J. E. Sim, R. Higashikubo, J. D. Baty, E. G. Moros and J. L. Roti Roti, Evaluation of parameters of oxidative stress after in vitro exposure to FMCW- and CDMA-modulated radiofrequency radiation fields. Radiat. Res. 162, 497-504 (2004).
41. M. Lantow, M. Lupke, J. Frahm, M. O. Mattsson, N. Kuster and M. Simko, ROS release and Hsp70 expression after exposure to 1,800 MHz radiofrequency electromagnetic fields in primary human monocytes and lymphocytes. Radiat. Environ. Biophys. 45, 55-62 (2006).
42. M. Lantow, J. Schuderer, C. Hartwig and M. Simko, Free radical release and HSP70 expression in two human immune-relevant cell lines after exposure to 1800 MHz radiofrequency radiation. Radiat. Res. 165, 88-94 (2006).
43. M. Simko, C. Hartwig, M. Lantow, M. Lupke, M. O. Mattsson, Q. Rahman and J. Rollwitz, Hsp70 expression and free radical release after exposure to non-thermal radio-frequency electromagnetic fields and ultrafine particles in human Mono Mac 6 cells. Toxicol. Lett. 161, 73-82 (2006).
44. O. Zeni, R. Di Pietro, G. d'Ambrosio, R. Massa, M. Capri, J. Naarala, J. Juutilainen and M. R. Scarfi, Formation of reactive oxygen species in L929 cells after exposure to 900 MHz RF radiation with and without co-exposure to 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone. Radiat. Res. 167, 306-311 (2007).
45. M. Zmyslony, P. Politanski, E. Rajkowska, W. Szymczak and J. Jajte, Acute exposure to 930 MHz CW electromagnetic radiation in vitro affects reactive oxygen species level in rat lymphocytes treated by iron ions. Bioelectromagnetics 25, 324-328 (2004).
46. A. Höytö, J. Juutilainen and J. Naarala, Ornithine decarboxylase activity is affected in primary astrocytes but not in secondary cell lines exposed to 872 MHz RF radiation. Int. J. Radiat. Biol. 83, 367-374 (2007).
47. A. Höytö, J. Juutilainen and J. Naarala, Ornithine decarboxylase activity of L929 cells after exposure to continuous wave or 50 Hz modulated radiofrequency radiation - a replication study. Bioelectromagnetics 28, 501-508 (2007).
48. M. Martínez-Búrdalo, A. Martín, M. Anguiano and R. Villar, Comparison of FDTD-calculated specific absorption rate in adults and children when using a mobile phone at 900 and 1800 MHz. Phys. Med. Biol. 49, 345-354 (2004).
49. A. Höytö, A. P. Sihvonen, L. Alhonen, J. Juutilainen and J. Naarala, Modest increase in temperature affects ODC activity in L929 cells: Low-level radiofrequency radiation does not. Radiat Environ. Biophys. 45, 231-235 (2006).
50. M. Takahashi, M. Shibata and E. Niki, Estimation of lipid peroxidation of live cells using a fluorescent probe, diphenyl-1-pyrenylphospine. Free Radic. Biol. Med. 31, 164-174 (2001).
51. M. Blank and Y. Shiloh, Programs for cell death: apoptosis is only one way to go. Cell Cycle 6, 686-695 (2007).
52. J. Huang, L. Wu, S. Tashiro, S. Onodera and T. A. Ikejima, A comparison of the signal pathways between the TNF alpha- and oridonin-induced murine L929 fibrosarcoma cell death. Acta Med. Okayama 59, 261-270 (2005).