In vitro exposure of human lymphocytes to 900 MHz CW and GSM modulated radio-frequency: Studies of proliferation, apoptosis and mitochondrial membrane potential

Radiation Research

Volumn 162, Issue 2, 2004, Pages 211-218

  Capri, Miriam ^a,e   Scarcella, Elena ^a   Fumelli, Cristiana ^a   Bianchi, Enrica ^a   Salvioli, Stefano ^a   Mesirca, Pietro ^a   Agostini, Chiara ^a   Antolini, Almerino ^b   Schiavoni, Andrea ^b   Castellani, Gastone ^a,e   Bersani, Ferdinando ^a,c,e   Franceschi, Claudio ^a,d,e  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b TELECOM ITALIA   (Italy)

^c UNIVERSITY OF GENOA   (Italy)

^d INRCA   (Italy)

^e Ctro Interdipartimentale L Galvani   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

2 DEOXYRIBOSE; PHOSPHATIDYLSERINE;

APOPTOSIS; ARTICLE; CELL CYCLE; CELL PROLIFERATION; CONTROLLED STUDY; HUMAN; HUMAN CELL; IMMUNOCOMPETENT CELL; LYMPHOCYTE; MEMBRANE POTENTIAL; MITOCHONDRIAL MEMBRANE; MOBILE PHONE; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; RADIATION ABSORPTION; RADIOFREQUENCY;

ADULT; APOPTOSIS; CELL DIVISION; HUMANS; LYMPHOCYTES; MEMBRANE POTENTIALS; MITOCHONDRIA;

EID: 3242882936     PISSN: 00337587     EISSN: None     Source Type: Journal    
DOI: 10.1667/RR3209     Document Type: Article

References (28)

1. J. Juutilainen and R. de Seze, Biological effects of amplitude-modulated radiofrequency radiation. Scand. J. Work Environ. Health 24, 245-254 (1998).
2. M. H. Repacholi, Low-level exposure to radiofrequency electromagnetic fields: Health effects and research needs. Bioelectromagnetics 19, 1-19 (1998).
3. M. H. Repacholi, Health risks from the use of mobile phones. Toxicol. Lett. 120, 323-331 (2001).
4. S. F. Cleary, Z. Du, G. Cao, L. M. Liu and C. McCrady, Effect of isothermal radiofrequency radiation on cytolytic T lymphocytes. FASEB J. 10, 913-919 (1996).
5. Vijayalaxmi, N. Mohan, M. L. Meltz and M. A. Wittler, Proliferation and cytogenetic studies in human blood lymphocytes exposed in vitro to 2450 MHz radiofrequency radiation. Int. J. Radiat. Biol. 72, 751-757 (1997).
6. Vijayalaxmi, B. Z. Leal, M. L. Meltz, W. F. Pickard, K. S. Bisht, J. L. Roti Roti, W. L. Straube and E. G. Moros, Cytogenetic studies in human blood lymphocytes exposed in vitro to radiofrequency radiation at a cellular telephone frequency (835.62 MHz, FDMA). Radiat. Res. 155, 113-121 (2001).
7. A. Schiavoni, P. Bertotto, G. Richiardi and P. Bielli, SAR generated by commercial cellular phones - Phone modelling, head modelling and measurements. IEEE Trans. MTT 48, 2064-2071 (2000).
8. G. D'Ambrosio, R. Massa, M. R. Scarfi and O. Zeni, Cytogenetic damage in human lymphocytes following GMSK phase modulated microwave exposure. Bioelectromagnetics 23, 7-13 (2002).
9. A. Boyum, Isolation of leucocytes from human blood. Further observations. Methylcellulose, dextran and ficoll as erythrocyteaggregating agents. Scand. J. Clin. Lab. Invest. 97 (Suppl.), 31-50 (1968).
10. M. L. Crawford, Generation of standard EM fields using TEM transmission cells. IEEE Trans. Electromagn. Compat. EMC-16, 189-195 (1974).
11. A. Taflove, Computational Electrodynamics. Artech House, Norwood, MA, 1995.
12. S. Berntsen and S. N. Hornsleth, Retarded time absorbing boundary conditions. IEEE Trans. Antennas Propagat. 42, 1059-1064 (1994).
13. C. Gabriel, S. Gabriel and E. Corthout, The dielectric properties of biological tissues: I. Literature survey. Phys. Med. Biol. 41, 2231-2249 (1996).
14. A. Cossarizza, D. Monti, F. Bersani, M. Cantini, R. Cadossi, A. Sacchi and C. Franceschi, Extremely low frequency pulsed electromagnetic fields increase cell proliferation in lymphocytes from young and aged subjects. Biochem. Biophys. Res. Commun. 160, 692-698 (1989).
15. F. Dolbeare, H. Gratzner, M. Pallavicini and J. W. Gray, Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine. Proc. Natl. Acad. Sci. USA 80, 5573-5577 (1983).
16. D. Barbieri, E. Grassilli, D. Monti, S. Salvioli, M. G. Franceschini, A. Franchini, E. Bellesia, P. Salomoni, M. Capri and C. Franceschi, D-Ribose and deoxy-D-ribose induce apoptosis in human quiescent peripheral blood mononuclear cells. Biochem. Biophys. Res. Commun. 201, 1109-1116 (1994).
17. D. Monti, S. Salvioli, M. Capri, W. Malori, E. Straface, A. Cossarizza, B. Botti, M. Piacentini, G. Baggio and C. Franceschi, Decreased susceptibility to oxidative stress-induced apoptosis of peripheral blood mononuclear cells from healthy elderly and centenarians. Mech. Ageing Dev. 121, 239-250 (2000).
18. G. Koopman, C. P. Reutelingsperger, G. A. Kuijten, R. M. Keehnen, S. T. Pals and M. H. van Oers, Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood 84, 1415-1420 (1994).
19. I. Vermes, C. Haanen, H. Steffens-Nakken and C. Reutelingsperger, A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J. Immunol. Methods 184, 39-51 (1995).
20. A. Cossarizza, G. Kalashnikova, E. Grassilli, F. Chiappelli, S. Salvioli, M. Capri, D. Barbieri, L. Troiano, D. Monti and C. Franceschi, Mitochondrial modifications during rat thymocyte apoptosis: A study at the single cell level. Exp. Cell. Res. 214, 323-330 (1994).
21. A. Cossarizza, M. Baccarani-Contri, G. Kalashnikova and C. Franceschi, A new method for the cytofluorimetric analysis of mitochondrial membrane potential using the J-aggregate-forming-lipophiliccation 5,5′,6,6′tetrachloro 1,1′,3,3′ tetraethylbenzimidazol carbo cyanine iodide (JC-1). Biochem. Biophys. Res. Commun. 197, 40-45 (1993).
22. S. Salvioli, A. Ardizzoni, C. Franceschi and A. Cossarizza, JC-1, but not DiOC6(3) or rhodamine 123, is a reliable fluorescent probe to assess delta psi changes in intact cells: Implications for studies on mitochondrial functionality during apoptosis. FEBS Lett. 411, 77-82 (1997).
23. P. W. French, M. Donnellan and D. R. McKenzie, Electromagnetic radiation at 835 MHz changes the morphology and inhibits proliferation of a human astrocytoma cell line. Bioelectrochem. Bioenerg. 43, 13-18 (1997).
24. M. Donnellan, D. R. McKenzie and P. W. French, Effects of exposure to electromagnetic radiation at 835 MHz on growth, morphology and secretory characteristics of a mast cell analogue, RBL-2H3. Cell. Biol. Interact. 21, 427-439 (1997).
25. S. Kwee and P Raskmark, Changes in cell proliferation due to environmental non-ionizing radiation 2. Microwave radiation. Bioelectrochem. Bioenerg. 44, 251-254 (1998).
26. S. Velizarov, P. Raskmark and S. Kwee, The effects of radiofrequency fields on cell proliferation are non-thermal. Bioelectrochem. Bioenerg. 48, 177-180 (1999).
27. G. J. Hook, P. Zhang, I. Lagroye, L. Li, R. Higashikubo, E. G. Moros, W. L. Straube, W. F. Pickard, J. D. Baty and J. L. Roti Roti, Measurement of DNA damage and apoptosis in Molt-4 cells after in vitro exposure to radiofrequency radiation. Radiat. Res. 161, 193-200 (2004).
28. E. Jaruga, S. Salvioli, J. Dobrucki, S. Chrul, J. Bandorowicz-Pikula, E. Sikora, C. Franceschi, A. Cossarizza and G. Bartosz, Apoptosis-like, reversible changes in plasma membrane asymmetry and permeability, and transient modifications in mitochondrial membrane potential induced by curcumin in rat thymocytes. FEBS Lett. 433, 287-293 (1998).