A versatile viral system for expression and depletion of proteins in mammalian cells

PLoS ONE

Volumn 4, Issue 8, 2009, Pages

  Campeau, Eric ^a   Ruhl, Victoria E ^a   Rodier, Francis ^b,c   Smith, Corey L ^a   Rahmberg, Brittany L ^a   Fuss, Jill O ^b   Campisi, Judith ^b,c   Yaswen, Paul ^b   Cooper, Priscilla K ^b   Kaufman, Paul D ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

^b LAWRENCE BERKELEY NATIONAL LABORATORY   (United States)

^c BUCK INSTITUTE FOR RESEARCH ON AGING   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AT RICH INTERACTING DOMAIN 4B PROTEIN; CELL PROTEIN; COMPLEMENTARY DNA; DOXYCYCLINE; HYBRID PROTEIN; LENTIVIRUS VECTOR; MICRORNA; RETROVIRUS VECTOR; SHORT HAIRPIN RNA; UNCLASSIFIED DRUG; VIRUS VECTOR; PRIMER DNA; PROTEIN; RNA;

ARTICLE; CELL AGING; CELL CYCLE ARREST; CELL CYCLE G1 PHASE; CELL LINE; DNA DAMAGE; DNA PURIFICATION; ELECTROPHORESIS; EXPRESSION VECTOR; FIBROBLAST; FLUORESCENCE ACTIVATED CELL SORTING; GENETIC RECOMBINATION; GENETIC TRANSFECTION; HELA CELL; HUMAN; IMMUNOFLUORESCENCE; LENTIVIRINAE; MAMMAL CELL; MITOSIS; MOLECULAR CLONING; NONHUMAN; PROTEIN DEPLETION; PROTEIN EXPRESSION; RETROVIRUS; RNA INTERFERENCE; SUPERNATANT; VIRUS TITRATION; ANIMAL; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; GENE VECTOR; GENETICS; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS;

MAMMALIA;

ANIMALS; BASE SEQUENCE; CELL LINE; DNA PRIMERS; DNA, COMPLEMENTARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; GENETIC VECTORS; HUMANS; PROTEINS; RECOMBINATION, GENETIC; RETROVIRIDAE; RNA; RNA INTERFERENCE;

EID: 68449083213     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0006529     Document Type: Article

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