One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-tag

Protein Expression and Purification

Volumn 23, Issue 3, 2001, Pages 440-446

  Keefe, Anthony D ^a   Wilson, David S ^a   Seelig, Burckhard ^a   Szostak, Jack W ^a  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA (MICROORGANISMS);

CARRIER PROTEIN; CHELATING AGENT; HYBRID PROTEIN; MALTOSE; STREPTAVIDIN; STREPTAVIDIN BINDING PEPTIDE; STREPTAVIDIN-BINDING PEPTIDE;

AFFINITY LABELING; AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; DNA VECTOR; ESCHERICHIA COLI; GENETIC TRANSFORMATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; NANOTECHNOLOGY; PROTEIN BINDING; RNA TRANSLATION; SENSITIVITY AND SPECIFICITY;

AFFINITY LABELS; AMINO ACID SEQUENCE; CARRIER PROTEINS; CHELATING AGENTS; ESCHERICHIA COLI; GENETIC VECTORS; MALTOSE; MOLECULAR SEQUENCE DATA; NANOTECHNOLOGY; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS; SENSITIVITY AND SPECIFICITY; STREPTAVIDIN; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, NON-P.H.S.; SUPPORT, U.S. GOV'T, P.H.S.; TRANSFORMATION, GENETIC; TRANSLATION, GENETIC;

EID: 0035543198     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1515     Document Type: Article

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