Systematic determination of the packaging limit of lentiviral vectors

Human Gene Therapy

Volumn 12, Issue 15, 2001, Pages 1893-1905

  Kumar, Mukesh ^a,b   Keller, Brian ^a   Makalou, Ndeye ^a,c   Sutton, Richard E ^a  

^a CENTER FOR CELL AND GENE THERAPY   (United States)

^b EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

^c UNIVERSITY OF TEXAS MD ANDERSON CANCER CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; DNA FRAGMENT; RESTRICTION ENDONUCLEASE; RNA; VIRUS PROTEIN; VIRUS VECTOR;

ARTICLE; BACTERIAL CHROMOSOME; CONTROLLED STUDY; DNA PACKAGING; GENE INSERTION; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; IMAGE ANALYSIS; LENTIVIRINAE; MAMMAL CELL; MOLECULAR CLONING; NONHUMAN; PROVIRUS; VESICULAR STOMATITIS VIRUS; VIRUS TITRATION;

ACTIVE TRANSPORT, CELL NUCLEUS; ANIMALS; BLOTTING, SOUTHERN; CAPSID; CELL LINE; DNA; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; HELA CELLS; HIV-1; HUMANS; LENTIVIRUS; MEMBRANE GLYCOPROTEINS; MODELS, GENETIC; PLASMIDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; TRANSDUCTION, GENETIC; TRANSFECTION; VIRAL ENVELOPE PROTEINS;

EID: 0034783643     PISSN: 10430342     EISSN: None     Source Type: Journal    
DOI: 10.1089/104303401753153947     Document Type: Article

References (18)

1. High efficiency gene transfer into CD34+ cells with a human immunodefiency virus type 1-based retroviral vector pseudotyped with vesicular stomatitis virus envelope glycoprotein G
2. Mutations of RNA and protein sequences involved in human immunodeficiency virus type 1 packaging result in production of noninfectious virus
3. A nuclear localization signal within HIV-1 matrix protein that governs infection of non-dividing cells
4. A mutant of human immunodeficiency virus with reduced RNA packaging and abnormal particle morphology
5. HIV-1 infection of non-dividing cells through the recognition of integrase by the importin/karyopherin pathway
6. The Vpr protein of human immunodeficiency virus type 1 influences nuclear localization of viral nucleic acids in non-dividing host cells
7. Nonreciprocal packaging of human immunodeficiency virus type 1 and type 2 RNA: A possible role for the p2 domain of Gag in RNA encapsidation
8. HIV RNA packaging and lentiviral-based vectors
9. Therapeutic haemoglobin synthesis in β-thalassaemic mice expressing lentivirus-encoding human β-globin
10. In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector
11. Analysis in human immunodeficiency virus type 1 vectors of cis-acting sequences that affect gene transfer into human lymphocytes
12. Analysis and cloning of eukaryotic genomic DNA
13. Broad host range of human T cell leukemia virus type 1 demonstrated with an improved pseudotyping system
14. Human immunodeficiency virus type 1 vectors efficiently transduce human hematopoietic stem cells
15. Synthesis, assembly and processing of viral proteins
16. Construction and use of a replication-competent human immunodeficiency virus (HIV-1) that expresses the chloramphenicol acetyltransferase enzyme
17. Lentiviral, but not oncoretroviral vectors mediate gene transfer into freshly isolated G0/G1 human hematopoietic stem cells
18. HIV-1 genome nuclear import is mediated by a central DNA flap